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The map label for this gene is prfA

Identifier: 218708755

GI number: 218708755

Start: 773124

End: 774212

Strand: Direct

Name: prfA

Synonym: VS_0752

Alternate gene names: 218708755

Gene position: 773124-774212 (Clockwise)

Preceding gene: 218708754

Following gene: 218708756

Centisome position: 23.43

GC content: 46.46

Gene sequence:

>1089_bases
ATGAAAGCCTCGATTCTAATAAAGCTTGAAACACTTGTTGAACGCTATGAAGAAGTTCAACATCTACTTGGTGATCCCGA
TGTACTTGGGAATCAAGATAAATTCCGTGCCTTGTCAAAAGAGTATTCTCAATTAGAAGAGGTGACGGGGTGCTTTAAAT
CATACCAGCAAGCTCAAGAAGATTTAGAAGCTGCTGAAGAGATGGCAAATGAAGACGACGCAGAAATGCGTGAAATGGCT
CAAGAAGAAATCAAAGATGCGAAAGCGAACATTGAACGTCTGACTGATGAGCTGCAAATTCTTCTGATTCCAAAAGATCC
AAACGATGAGCGTAACTGCTTCTTAGAGATTCGTGCCGGCGCAGGTGGTGATGAAGCGGGTATCTTTGCGGGTAACCTTT
TCCGTATGTACTCTAAGTTTGCCGAGAAAAAAGGCTGGCGCGTCGAAGTGATGAGCAGCAATGTTTCAGAGCAGGGCGGC
TTTAAAGAGATGATCGCTAAAATCAGTGGCGATGCTGTTTACGGCACAATGAAGTTCGAGTCTGGTGGTCACCGTGTACA
ACGTGTACCTGAGACTGAATCACAAGGTCGTGTTCATACGTCTGCATGTACCGTTGCGGTTATGCCTGAGATTCCAGAAG
CAGATCTGCCAGAGATCAAAGCTGGCGACCTTAAGATTGATACTTTCCGTGCATCTGGCGCGGGTGGTCAGCACGTTAAC
ACCACGGATTCAGCAATCCGTATTACTCACTTACCAACAGGTACAGTAGTAGAGTGTCAAGACGAGCGTTCTCAGCATAA
AAACAAAGCGAAAGCGATGGCTGTTCTTGCGGCTCGTATCGTTCAAGCTGAAGAAGAGCGTCGTGCTGCTGCGATTTCAG
ATACACGTCGTAACCTATTAGGTTCTGGTGACCGTAGTGACCGTATTCGTACGTACAACTACCCTCAAGGCCGTGTTTCT
GATCACCGCATCAACTTAACTATCTACCGCCTGAACGAAGTTCTGGAAGGTGATATGCAAAGCCTGCTTGATCCTGTTCT
ACAAGAGCACCAAGCCGATCAACTAGCAGCACTTGCTGAGCACAACTAA

Upstream 100 bases:

>100_bases
GCAGCAGAGCAAGGAGAACCTGCAAAATTAACGGTCATTAGACAGAGTTTGGGCTTAGAAAACCCTCAATAATATTCGAC
CTCCAACAGAATAAGACATT

Downstream 100 bases:

>100_bases
TTACTATGCAGTCAGCATATACGGTTGAAAGTGCTTTAAAGTCAGCAATCGTACAGCTTCAAGAGGGCGATAACACATCG
CCCTCGATTGATGCTACGGT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MKASILIKLETLVERYEEVQHLLGDPDVLGNQDKFRALSKEYSQLEEVTGCFKSYQQAQEDLEAAEEMANEDDAEMREMA
QEEIKDAKANIERLTDELQILLIPKDPNDERNCFLEIRAGAGGDEAGIFAGNLFRMYSKFAEKKGWRVEVMSSNVSEQGG
FKEMIAKISGDAVYGTMKFESGGHRVQRVPETESQGRVHTSACTVAVMPEIPEADLPEIKAGDLKIDTFRASGAGGQHVN
TTDSAIRITHLPTGTVVECQDERSQHKNKAKAMAVLAARIVQAEEERRAAAISDTRRNLLGSGDRSDRIRTYNYPQGRVS
DHRINLTIYRLNEVLEGDMQSLLDPVLQEHQADQLAALAEHN

Sequences:

>Translated_362_residues
MKASILIKLETLVERYEEVQHLLGDPDVLGNQDKFRALSKEYSQLEEVTGCFKSYQQAQEDLEAAEEMANEDDAEMREMA
QEEIKDAKANIERLTDELQILLIPKDPNDERNCFLEIRAGAGGDEAGIFAGNLFRMYSKFAEKKGWRVEVMSSNVSEQGG
FKEMIAKISGDAVYGTMKFESGGHRVQRVPETESQGRVHTSACTVAVMPEIPEADLPEIKAGDLKIDTFRASGAGGQHVN
TTDSAIRITHLPTGTVVECQDERSQHKNKAKAMAVLAARIVQAEEERRAAAISDTRRNLLGSGDRSDRIRTYNYPQGRVS
DHRINLTIYRLNEVLEGDMQSLLDPVLQEHQADQLAALAEHN
>Mature_362_residues
MKASILIKLETLVERYEEVQHLLGDPDVLGNQDKFRALSKEYSQLEEVTGCFKSYQQAQEDLEAAEEMANEDDAEMREMA
QEEIKDAKANIERLTDELQILLIPKDPNDERNCFLEIRAGAGGDEAGIFAGNLFRMYSKFAEKKGWRVEVMSSNVSEQGG
FKEMIAKISGDAVYGTMKFESGGHRVQRVPETESQGRVHTSACTVAVMPEIPEADLPEIKAGDLKIDTFRASGAGGQHVN
TTDSAIRITHLPTGTVVECQDERSQHKNKAKAMAVLAARIVQAEEERRAAAISDTRRNLLGSGDRSDRIRTYNYPQGRVS
DHRINLTIYRLNEVLEGDMQSLLDPVLQEHQADQLAALAEHN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=302, Percent_Identity=45.6953642384106, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI34577120, Length=361, Percent_Identity=37.6731301939058, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=45.4106280193237, Blast_Score=190, Evalue=2e-48,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=69.7222222222222, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=38.1656804733728, Blast_Score=217, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17542784, Length=290, Percent_Identity=36.8965517241379, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321295, Length=307, Percent_Identity=41.042345276873, Blast_Score=241, Evalue=1e-64,
Organism=Drosophila melanogaster, GI19921226, Length=328, Percent_Identity=40.2439024390244, Blast_Score=225, Evalue=3e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_VIBSL (B7VKH3)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416376.1
- ProteinModelPortal:   B7VKH3
- SMR:   B7VKH3
- GeneID:   7160353
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0752
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40365; Mature: 40365

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASILIKLETLVERYEEVQHLLGDPDVLGNQDKFRALSKEYSQLEEVTGCFKSYQQAQE
CCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLEAAEEMANEDDAEMREMAQEEIKDAKANIERLTDELQILLIPKDPNDERNCFLEIRAG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEECC
AGGDEAGIFAGNLFRMYSKFAEKKGWRVEVMSSNVSEQGGFKEMIAKISGDAVYGTMKFE
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCCEEEEEEEEC
SGGHRVQRVPETESQGRVHTSACTVAVMPEIPEADLPEIKAGDLKIDTFRASGAGGQHVN
CCCCHHHCCCCCCCCCCEEECEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
TTDSAIRITHLPTGTVVECQDERSQHKNKAKAMAVLAARIVQAEEERRAAAISDTRRNLL
CCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSGDRSDRIRTYNYPQGRVSDHRINLTIYRLNEVLEGDMQSLLDPVLQEHQADQLAALAE
CCCCCCCCCEECCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
HN
CC
>Mature Secondary Structure
MKASILIKLETLVERYEEVQHLLGDPDVLGNQDKFRALSKEYSQLEEVTGCFKSYQQAQE
CCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLEAAEEMANEDDAEMREMAQEEIKDAKANIERLTDELQILLIPKDPNDERNCFLEIRAG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEECC
AGGDEAGIFAGNLFRMYSKFAEKKGWRVEVMSSNVSEQGGFKEMIAKISGDAVYGTMKFE
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCCEEEEEEEEC
SGGHRVQRVPETESQGRVHTSACTVAVMPEIPEADLPEIKAGDLKIDTFRASGAGGQHVN
CCCCHHHCCCCCCCCCCEEECEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
TTDSAIRITHLPTGTVVECQDERSQHKNKAKAMAVLAARIVQAEEERRAAAISDTRRNLL
CCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSGDRSDRIRTYNYPQGRVSDHRINLTIYRLNEVLEGDMQSLLDPVLQEHQADQLAALAE
CCCCCCCCCEECCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
HN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA