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The map label for this gene is miaB [H]

Identifier: 218708745

GI number: 218708745

Start: 760273

End: 761697

Strand: Reverse

Name: miaB [H]

Synonym: VS_0724

Alternate gene names: 218708745

Gene position: 761697-760273 (Counterclockwise)

Preceding gene: 218708747

Following gene: 218708744

Centisome position: 23.09

GC content: 44.77

Gene sequence:

>1425_bases
ATGAGTAAGAAACTGCTAATTAAAACTTGGGGCTGTCAGATGAATGAATATGATTCATCAAAAATGGCCGACCTGCTTAA
CGCTGCAAATGGCTATGAGCTAACTGAAGTACCTGAGGAAGCAGACGTACTACTATTGAATACTTGTTCTATCCGCGAAA
AAGCGCAAGAAAAAGTATTCCACCAGCTTGGTCGTTGGAAAACGCTTAAAGATAAAAAAGAAGGTGTGGTGATCGGTGTG
GGTGGCTGCGTAGCGACTCAAGAAGGTGATCACATTCGTCAACGTGCACCATACGTAGACGTTATCTTTGGCCCACAAAC
ATTGCACCGTTTGCCAGAGATGATTAAGTCATCACTGTCTAACGAAAAGCCAGTAATGGACATCTCTTTCCCAGAGATCG
AAAAGTTCGATAACCTGCCCGAGCCAAAAGCTGAAGGCGCGACGGCGTTCGTTTCTATCATGGAAGGTTGTTCTAAATAC
TGTACTTACTGCGTTGTACCATACACACGTGGTGAAGAAGTTAGCCGTCCAATGGATGATGTACTTTTCGAAGTTGCTCA
ACTTGCAGAGCAAGGCGTACGTGAAGTTAACCTGCTAGGTCAAAACGTAAACGCATACCGCGGTCCAACTCACGAAGGTG
ATATCTGTTCATTCGCAGAACTGCTTCGTCTTGTTGCTTCTATCGATGGTATCGACCGTATTCGTTTCACAACAAGCCAC
CCGCTTGAGTTTGGTGACGACATCATTGAAGTTTACAAAGATACACCAGAACTAGTGAGCTTCCTTCACCTACCAGTACA
AAGTGGTAGTGACCGTATTCTTACGATGATGAAGCGCCCACATACGGCTATCGAGTACAAATCTATTATCCGTAAACTGC
GTAAAGCTCGTCCTGACATTCAAATCAGCTCTGACTTTATTGTTGGTTTCCCTGGTGAGTCTAAGCAAGACTTCCAAGAT
ACAATGAAGCTAATTAAAGAAGTTGATTTCGATATGAGCTTCAGCTTTGTTTTCTCTCCACGTCCAGGTACGCCAGCGGC
AGATTACCCATGTGATGTACCAGCACAAGAGAAGAAAGATCGTCTGTACGAACTGCAGCAAACGGTAAACACACAAGCTA
TGCGTTTCTCTCGTCTAATGTTAGGCACAGAGCAACGTATCCTTGTTGAAGGCCCATCAAGAAAGAACCTAATGGAACTT
CGCGGCCGTACAGAAAACAGCCGTGTTGTGAACTTCGAAGGTTCTGCAGACCTAATCGGCCAGTTCGTAGATGTGAAGAT
CACTGAGGTTTACACCAACTCACTACGTGGTGAGCTAGTTCGCACAGAAAAAGACATGGGTCTACGCGTTGTTATGACTC
CAGCAGAAATGATGGAAAAAACTAAACGTGAAGACGAGCTAGGTGTAGCAACATTTACGCCATAG

Upstream 100 bases:

>100_bases
TGTCATAAAATTGAATACCGAATTATGACAAACGGGGCTAGCGGATGAATAATGGGCGATTTGCTCCCTTAAATTAAACT
AACGATCGAGCGAACATATA

Downstream 100 bases:

>100_bases
GCAATACAACTGTTTGAGCACAAAGCGTAAACACAAAGCTTGAAATTACCTAACTAAGTGACCATCTTAGAAATAGGGAT
ATCACCATCAGAAGCCCGGT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSKKLLIKTWGCQMNEYDSSKMADLLNAANGYELTEVPEEADVLLLNTCSIREKAQEKVFHQLGRWKTLKDKKEGVVIGV
GGCVATQEGDHIRQRAPYVDVIFGPQTLHRLPEMIKSSLSNEKPVMDISFPEIEKFDNLPEPKAEGATAFVSIMEGCSKY
CTYCVVPYTRGEEVSRPMDDVLFEVAQLAEQGVREVNLLGQNVNAYRGPTHEGDICSFAELLRLVASIDGIDRIRFTTSH
PLEFGDDIIEVYKDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDIQISSDFIVGFPGESKQDFQD
TMKLIKEVDFDMSFSFVFSPRPGTPAADYPCDVPAQEKKDRLYELQQTVNTQAMRFSRLMLGTEQRILVEGPSRKNLMEL
RGRTENSRVVNFEGSADLIGQFVDVKITEVYTNSLRGELVRTEKDMGLRVVMTPAEMMEKTKREDELGVATFTP

Sequences:

>Translated_474_residues
MSKKLLIKTWGCQMNEYDSSKMADLLNAANGYELTEVPEEADVLLLNTCSIREKAQEKVFHQLGRWKTLKDKKEGVVIGV
GGCVATQEGDHIRQRAPYVDVIFGPQTLHRLPEMIKSSLSNEKPVMDISFPEIEKFDNLPEPKAEGATAFVSIMEGCSKY
CTYCVVPYTRGEEVSRPMDDVLFEVAQLAEQGVREVNLLGQNVNAYRGPTHEGDICSFAELLRLVASIDGIDRIRFTTSH
PLEFGDDIIEVYKDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDIQISSDFIVGFPGESKQDFQD
TMKLIKEVDFDMSFSFVFSPRPGTPAADYPCDVPAQEKKDRLYELQQTVNTQAMRFSRLMLGTEQRILVEGPSRKNLMEL
RGRTENSRVVNFEGSADLIGQFVDVKITEVYTNSLRGELVRTEKDMGLRVVMTPAEMMEKTKREDELGVATFTP
>Mature_473_residues
SKKLLIKTWGCQMNEYDSSKMADLLNAANGYELTEVPEEADVLLLNTCSIREKAQEKVFHQLGRWKTLKDKKEGVVIGVG
GCVATQEGDHIRQRAPYVDVIFGPQTLHRLPEMIKSSLSNEKPVMDISFPEIEKFDNLPEPKAEGATAFVSIMEGCSKYC
TYCVVPYTRGEEVSRPMDDVLFEVAQLAEQGVREVNLLGQNVNAYRGPTHEGDICSFAELLRLVASIDGIDRIRFTTSHP
LEFGDDIIEVYKDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDIQISSDFIVGFPGESKQDFQDT
MKLIKEVDFDMSFSFVFSPRPGTPAADYPCDVPAQEKKDRLYELQQTVNTQAMRFSRLMLGTEQRILVEGPSRKNLMELR
GRTENSRVVNFEGSADLIGQFVDVKITEVYTNSLRGELVRTEKDMGLRVVMTPAEMMEKTKREDELGVATFTP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=481, Percent_Identity=35.1351351351351, Blast_Score=280, Evalue=3e-75,
Organism=Homo sapiens, GI28872782, Length=481, Percent_Identity=35.1351351351351, Blast_Score=279, Evalue=5e-75,
Organism=Homo sapiens, GI93277076, Length=456, Percent_Identity=30.7017543859649, Blast_Score=188, Evalue=9e-48,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=76.1603375527426, Blast_Score=766, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=383, Percent_Identity=29.5039164490862, Blast_Score=164, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI17553146, Length=461, Percent_Identity=32.3210412147505, Blast_Score=250, Evalue=9e-67,
Organism=Caenorhabditis elegans, GI71996771, Length=350, Percent_Identity=26.5714285714286, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21356207, Length=475, Percent_Identity=35.7894736842105, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI19922432, Length=472, Percent_Identity=31.1440677966102, Blast_Score=170, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 53591; Mature: 53460

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKLLIKTWGCQMNEYDSSKMADLLNAANGYELTEVPEEADVLLLNTCSIREKAQEKVF
CCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHH
HQLGRWKTLKDKKEGVVIGVGGCVATQEGDHIRQRAPYVDVIFGPQTLHRLPEMIKSSLS
HHHHCCCCCCCCCCCEEEECCCEEECCCCCHHHHHCCEEEEEECHHHHHHHHHHHHHHHC
NEKPVMDISFPEIEKFDNLPEPKAEGATAFVSIMEGCSKYCTYCVVPYTRGEEVSRPMDD
CCCCEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCHHCCCHHH
VLFEVAQLAEQGVREVNLLGQNVNAYRGPTHEGDICSFAELLRLVASIDGIDRIRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCC
PLEFGDDIIEVYKDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDI
CCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
QISSDFIVGFPGESKQDFQDTMKLIKEVDFDMSFSFVFSPRPGTPAADYPCDVPAQEKKD
EECCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHH
RLYELQQTVNTQAMRFSRLMLGTEQRILVEGPSRKNLMELRGRTENSRVVNFEGSADLIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCCCCEEEEECCCHHHHH
QFVDVKITEVYTNSLRGELVRTEKDMGLRVVMTPAEMMEKTKREDELGVATFTP
HHHCEEEHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHCCHHHCCCEECCC
>Mature Secondary Structure 
SKKLLIKTWGCQMNEYDSSKMADLLNAANGYELTEVPEEADVLLLNTCSIREKAQEKVF
CCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHH
HQLGRWKTLKDKKEGVVIGVGGCVATQEGDHIRQRAPYVDVIFGPQTLHRLPEMIKSSLS
HHHHCCCCCCCCCCCEEEECCCEEECCCCCHHHHHCCEEEEEECHHHHHHHHHHHHHHHC
NEKPVMDISFPEIEKFDNLPEPKAEGATAFVSIMEGCSKYCTYCVVPYTRGEEVSRPMDD
CCCCEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCHHCCCHHH
VLFEVAQLAEQGVREVNLLGQNVNAYRGPTHEGDICSFAELLRLVASIDGIDRIRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCC
PLEFGDDIIEVYKDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDI
CCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
QISSDFIVGFPGESKQDFQDTMKLIKEVDFDMSFSFVFSPRPGTPAADYPCDVPAQEKKD
EECCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHH
RLYELQQTVNTQAMRFSRLMLGTEQRILVEGPSRKNLMELRGRTENSRVVNFEGSADLIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCCCCEEEEECCCHHHHH
QFVDVKITEVYTNSLRGELVRTEKDMGLRVVMTPAEMMEKTKREDELGVATFTP
HHHCEEEHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHCCHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA