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The map label for this gene is mrdA [H]

Identifier: 218708734

GI number: 218708734

Start: 747950

End: 749842

Strand: Reverse

Name: mrdA [H]

Synonym: VS_0713

Alternate gene names: 218708734

Gene position: 749842-747950 (Counterclockwise)

Preceding gene: 218708735

Following gene: 218708733

Centisome position: 22.73

GC content: 44.8

Gene sequence:

>1893_bases
ATGTTACGTAAACGTAGCCAAATTCGTGATTACAAAGCAGAAGCACGACTATTTACTAGTCGTGCTTTTGTTGCGTTTGC
GGGGATTATAGTGATGATGAGCTTACTGGTTGTGAACCTTTATAACATCCAAGTTAATCAATATCAGGACTATAAAACCC
GCTCCAACGACAATCGCATCAAGGTTGTTCCAATAGCACCTAACCGTGGTTTAATTTACGACCGCAACGGTGTACTTCTT
GCAGAAAACCGGCCTGTTTTTAATCTGGAGATCACCCCAGAAAAAGTCAAGAACATGGAAGATACGCTTGTTCGTTTACA
AGCGTTAATTGACATACCACCGGAGCGTATCGAACGTTTTAATCGTGATCGTCGTAATTCACGTCGCTTCAAATCAGTAC
CTATTCTAAATCAGCTCACCGAAGAACAAGTGGCGGTCTTTTCTGTAAATCAACATAAGTTCCCAGGCGTTGAAGTGACA
GGTACGCTAAAACGTTTCTACCCTTATGGTGATGTGTTAACTCACGTAATTGGCTACGTATCGCGCATTAATGACCGTGA
TATGCAGCGCTTGGTTCGTGAAGAGAAAGACGCCAATTACCAAGCTACCCGCGATATCGGTAAACTCGGCATTGAGCGCT
ATTACGAAGATATGCTACACGGCACCGCTGGCTATCAAGAAGTCGAAGTAAACAGTCGTGGACGAGTGATCCGCACTCTT
AAATTTGTGCCTTCGGCTCCGGGCAAAGATATCGTGCTCAACTTGGATATCAAACTGCAGCTATACGTACATAAGTTACT
TGATGGCCGCCGTGGTTCAGCCGTGGTTCTCGACCCGAAAGACAATGGCGTATTAGCCATGGTTTCCAGCCCAAGCTACG
ACCCAAATGCCTTTGTGCATGGTATTTCCTCGAAAGGTTATAATGCCCTGCTTCAAGACAAAGACCGACCTTTAGTTAAC
CGCGCAACGCTAGGAATTTATCCGCCGGCATCGACGATCAAACCCTTCATTGCAGTAGCAGCTCTGCAAGAAGGCGTAAT
TACTCCGAACACTACACGTAACGATCCTGGTTATTGGAAGATACCCAATTCGAAAACGAAACCATTTCGAGACTGGTTGC
GCTGGGGACACGGTGTCGTTGACATAGAGAAAGCCATTGAAGAATCAGTTGATACCTTCTTCTACCAAATTTCATTTGAC
TTAGGCATCGATCGCTTATCTAAATGGATGATGTTATTCGGTTTTGGTGATTACACTGGCATCGACATTTACGAAGAAAG
CAAAGCGAATATGCCCACTCGCGAATGGAAAATGGCCAGACACCGTGTACCTTGGTATCAAGGTGACACCATCCCTGTCG
GTATTGGCCAGGGCTACTGGACAGCAACACCAATGCAGATTGCAAAAGCGACTTCAGTATTGGTCAATGAAGGAGAGGTA
ACCGCTCCCCATTTACTACGCGCGACAATTGAGAACGGTCAACCGTTCGATGAACAAACGATATCTGAAATCGAAACGTA
CCCTCCGGTAACTGGTGTTAAGCAGAGATACTGGGATATCGCGCAAGAAGGCATGAGATTGGTAAACCACGGAAAGAAAG
GCACAGCAAGACGCTCATTCCAAAAAATGTCTTATCAAACTGCTGGTAAATCAGGTACTGCACAAGTATTCGGTCTAAAA
GAAGATGAAGAATACAACGCAGATGAAATCGCAGAACATTTACGCGATCATGCCCTCTTCACCGGTTATGCGCCTTTTGA
AGATCCTGAAGCGGTCGTAACTATCGTTTTGGAGAATGCCGGTGGTGGTTCATCAAATGGTGGCCCAGTTGTAAGAAGAA
TTTTAGACCATATTATTCTTGCAGAAGATTATGAAAGTGAGCCAATAAAATAA

Upstream 100 bases:

>100_bases
TACTCTCCCTCACCCATTAGTACGCGTTATCATGGCTGAAAGCTTGTATCGAGCTTGGAGCGTCACTGCTAACCACCCTT
ATCATCGAGAATAAGCGTTA

Downstream 100 bases:

>100_bases
TGAAACTTGATCCTTCAACTGGACGAAATAGAGCCCTATTTGAACGGCTGCATATCGACCTGCCGCTATTGCTTGGCATT
CTGGTTTTAATGGGCTTTGC

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 630; Mature: 630

Protein sequence:

>630_residues
MLRKRSQIRDYKAEARLFTSRAFVAFAGIIVMMSLLVVNLYNIQVNQYQDYKTRSNDNRIKVVPIAPNRGLIYDRNGVLL
AENRPVFNLEITPEKVKNMEDTLVRLQALIDIPPERIERFNRDRRNSRRFKSVPILNQLTEEQVAVFSVNQHKFPGVEVT
GTLKRFYPYGDVLTHVIGYVSRINDRDMQRLVREEKDANYQATRDIGKLGIERYYEDMLHGTAGYQEVEVNSRGRVIRTL
KFVPSAPGKDIVLNLDIKLQLYVHKLLDGRRGSAVVLDPKDNGVLAMVSSPSYDPNAFVHGISSKGYNALLQDKDRPLVN
RATLGIYPPASTIKPFIAVAALQEGVITPNTTRNDPGYWKIPNSKTKPFRDWLRWGHGVVDIEKAIEESVDTFFYQISFD
LGIDRLSKWMMLFGFGDYTGIDIYEESKANMPTREWKMARHRVPWYQGDTIPVGIGQGYWTATPMQIAKATSVLVNEGEV
TAPHLLRATIENGQPFDEQTISEIETYPPVTGVKQRYWDIAQEGMRLVNHGKKGTARRSFQKMSYQTAGKSGTAQVFGLK
EDEEYNADEIAEHLRDHALFTGYAPFEDPEAVVTIVLENAGGGSSNGGPVVRRILDHIILAEDYESEPIK

Sequences:

>Translated_630_residues
MLRKRSQIRDYKAEARLFTSRAFVAFAGIIVMMSLLVVNLYNIQVNQYQDYKTRSNDNRIKVVPIAPNRGLIYDRNGVLL
AENRPVFNLEITPEKVKNMEDTLVRLQALIDIPPERIERFNRDRRNSRRFKSVPILNQLTEEQVAVFSVNQHKFPGVEVT
GTLKRFYPYGDVLTHVIGYVSRINDRDMQRLVREEKDANYQATRDIGKLGIERYYEDMLHGTAGYQEVEVNSRGRVIRTL
KFVPSAPGKDIVLNLDIKLQLYVHKLLDGRRGSAVVLDPKDNGVLAMVSSPSYDPNAFVHGISSKGYNALLQDKDRPLVN
RATLGIYPPASTIKPFIAVAALQEGVITPNTTRNDPGYWKIPNSKTKPFRDWLRWGHGVVDIEKAIEESVDTFFYQISFD
LGIDRLSKWMMLFGFGDYTGIDIYEESKANMPTREWKMARHRVPWYQGDTIPVGIGQGYWTATPMQIAKATSVLVNEGEV
TAPHLLRATIENGQPFDEQTISEIETYPPVTGVKQRYWDIAQEGMRLVNHGKKGTARRSFQKMSYQTAGKSGTAQVFGLK
EDEEYNADEIAEHLRDHALFTGYAPFEDPEAVVTIVLENAGGGSSNGGPVVRRILDHIILAEDYESEPIK
>Mature_630_residues
MLRKRSQIRDYKAEARLFTSRAFVAFAGIIVMMSLLVVNLYNIQVNQYQDYKTRSNDNRIKVVPIAPNRGLIYDRNGVLL
AENRPVFNLEITPEKVKNMEDTLVRLQALIDIPPERIERFNRDRRNSRRFKSVPILNQLTEEQVAVFSVNQHKFPGVEVT
GTLKRFYPYGDVLTHVIGYVSRINDRDMQRLVREEKDANYQATRDIGKLGIERYYEDMLHGTAGYQEVEVNSRGRVIRTL
KFVPSAPGKDIVLNLDIKLQLYVHKLLDGRRGSAVVLDPKDNGVLAMVSSPSYDPNAFVHGISSKGYNALLQDKDRPLVN
RATLGIYPPASTIKPFIAVAALQEGVITPNTTRNDPGYWKIPNSKTKPFRDWLRWGHGVVDIEKAIEESVDTFFYQISFD
LGIDRLSKWMMLFGFGDYTGIDIYEESKANMPTREWKMARHRVPWYQGDTIPVGIGQGYWTATPMQIAKATSVLVNEGEV
TAPHLLRATIENGQPFDEQTISEIETYPPVTGVKQRYWDIAQEGMRLVNHGKKGTARRSFQKMSYQTAGKSGTAQVFGLK
EDEEYNADEIAEHLRDHALFTGYAPFEDPEAVVTIVLENAGGGSSNGGPVVRRILDHIILAEDYESEPIK

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=627, Percent_Identity=56.4593301435407, Blast_Score=747, Evalue=0.0,
Organism=Escherichia coli, GI1786272, Length=629, Percent_Identity=24.6422893481717, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 71508; Mature: 71508

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRKRSQIRDYKAEARLFTSRAFVAFAGIIVMMSLLVVNLYNIQVNQYQDYKTRSNDNRI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEE
KVVPIAPNRGLIYDRNGVLLAENRPVFNLEITPEKVKNMEDTLVRLQALIDIPPERIERF
EEEEECCCCCEEECCCCEEEECCCCEEEEEECHHHHHCHHHHHHHHHHHHCCCHHHHHHH
NRDRRNSRRFKSVPILNQLTEEQVAVFSVNQHKFPGVEVTGTLKRFYPYGDVLTHVIGYV
HHHHHHCCHHCCCCHHHHHHHHHEEEEEECCCCCCCEEEEEHHHHHCCCHHHHHHHHHHH
SRINDRDMQRLVREEKDANYQATRDIGKLGIERYYEDMLHGTAGYQEVEVNSRGRVIRTL
HHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEE
KFVPSAPGKDIVLNLDIKLQLYVHKLLDGRRGSAVVLDPKDNGVLAMVSSPSYDPNAFVH
EECCCCCCCCEEEEEEEEEHHHHHHHHCCCCCCEEEECCCCCCEEEEEECCCCCCCCEEE
GISSKGYNALLQDKDRPLVNRATLGIYPPASTIKPFIAVAALQEGVITPNTTRNDPGYWK
ECCCCCCHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
IPNSKTKPFRDWLRWGHGVVDIEKAIEESVDTFFYQISFDLGIDRLSKWMMLFGFGDYTG
CCCCCCCHHHHHHHHCCCEEHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHCCCCCCC
IDIYEESKANMPTREWKMARHRVPWYQGDTIPVGIGQGYWTATPMQIAKATSVLVNEGEV
CEEEECCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHEEECCCC
TAPHLLRATIENGQPFDEQTISEIETYPPVTGVKQRYWDIAQEGMRLVNHGKKGTARRSF
CCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHH
QKMSYQTAGKSGTAQVFGLKEDEEYNADEIAEHLRDHALFTGYAPFEDPEAVVTIVLENA
HHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCEEECCCCCCCCCEEEEEEEECC
GGGSSNGGPVVRRILDHIILAEDYESEPIK
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLRKRSQIRDYKAEARLFTSRAFVAFAGIIVMMSLLVVNLYNIQVNQYQDYKTRSNDNRI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEE
KVVPIAPNRGLIYDRNGVLLAENRPVFNLEITPEKVKNMEDTLVRLQALIDIPPERIERF
EEEEECCCCCEEECCCCEEEECCCCEEEEEECHHHHHCHHHHHHHHHHHHCCCHHHHHHH
NRDRRNSRRFKSVPILNQLTEEQVAVFSVNQHKFPGVEVTGTLKRFYPYGDVLTHVIGYV
HHHHHHCCHHCCCCHHHHHHHHHEEEEEECCCCCCCEEEEEHHHHHCCCHHHHHHHHHHH
SRINDRDMQRLVREEKDANYQATRDIGKLGIERYYEDMLHGTAGYQEVEVNSRGRVIRTL
HHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEE
KFVPSAPGKDIVLNLDIKLQLYVHKLLDGRRGSAVVLDPKDNGVLAMVSSPSYDPNAFVH
EECCCCCCCCEEEEEEEEEHHHHHHHHCCCCCCEEEECCCCCCEEEEEECCCCCCCCEEE
GISSKGYNALLQDKDRPLVNRATLGIYPPASTIKPFIAVAALQEGVITPNTTRNDPGYWK
ECCCCCCHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
IPNSKTKPFRDWLRWGHGVVDIEKAIEESVDTFFYQISFDLGIDRLSKWMMLFGFGDYTG
CCCCCCCHHHHHHHHCCCEEHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHCCCCCCC
IDIYEESKANMPTREWKMARHRVPWYQGDTIPVGIGQGYWTATPMQIAKATSVLVNEGEV
CEEEECCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHEEECCCC
TAPHLLRATIENGQPFDEQTISEIETYPPVTGVKQRYWDIAQEGMRLVNHGKKGTARRSF
CCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHH
QKMSYQTAGKSGTAQVFGLKEDEEYNADEIAEHLRDHALFTGYAPFEDPEAVVTIVLENA
HHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCEEECCCCCCCCCEEEEEEEECC
GGGSSNGGPVVRRILDHIILAEDYESEPIK
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]