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The map label for this gene is rlmH [H]

Identifier: 218708735

GI number: 218708735

Start: 749849

End: 750319

Strand: Reverse

Name: rlmH [H]

Synonym: VS_0714

Alternate gene names: 218708735

Gene position: 750319-749849 (Counterclockwise)

Preceding gene: 218708736

Following gene: 218708734

Centisome position: 22.74

GC content: 45.86

Gene sequence:

>471_bases
TTGAAGATCCAGTTAATCGCAGTTGGTACAAAAATGCCAAAGTGGGTTGAAGAAGGCTTTAAAGAATATAAACGCCGCTT
CCCTCATGATATGCCATTAGAACTCATTGAAATCACGGCGGGAAAACGCGGTAAAAATGCCGATATTGCACGTATTCTTC
AAAAAGAAGGCGAAGCGATGTTAGCTGCAGTTCCAAAAGGTAACCGCATTATTACACTCGATATCCCAGGTAAAAAGTGG
GATACGCCTCAACTGGCAGAGCAATTGGAAAGTTGGAAGCTGGATGGCCGTGACGTTTCTATCCTTATTGGCGGGCCTGA
AGGATTAGCACCAGCATGTAAAGCGGCAGCAGACCAAAGCTGGTCTCTGTCAGCACTTACTCTCCCTCACCCATTAGTAC
GCGTTATCATGGCTGAAAGCTTGTATCGAGCTTGGAGCGTCACTGCTAACCACCCTTATCATCGAGAATAA

Upstream 100 bases:

>100_bases
AGGTGAATGGGTTGTTCTAGACATGGGCACAAGCATGCTTCACGTTATGCAAGAAGAGCATCGTGAACTGTACCAACTAG
AAAAACTTTGGGGCTAAGCT

Downstream 100 bases:

>100_bases
GCGTTAATGTTACGTAAACGTAGCCAAATTCGTGATTACAAAGCAGAAGCACGACTATTTACTAGTCGTGCTTTTGTTGC
GTTTGCGGGGATTATAGTGA

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKIQLIAVGTKMPKWVEEGFKEYKRRFPHDMPLELIEITAGKRGKNADIARILQKEGEAMLAAVPKGNRIITLDIPGKKW
DTPQLAEQLESWKLDGRDVSILIGGPEGLAPACKAAADQSWSLSALTLPHPLVRVIMAESLYRAWSVTANHPYHRE

Sequences:

>Translated_156_residues
MKIQLIAVGTKMPKWVEEGFKEYKRRFPHDMPLELIEITAGKRGKNADIARILQKEGEAMLAAVPKGNRIITLDIPGKKW
DTPQLAEQLESWKLDGRDVSILIGGPEGLAPACKAAADQSWSLSALTLPHPLVRVIMAESLYRAWSVTANHPYHRE
>Mature_156_residues
MKIQLIAVGTKMPKWVEEGFKEYKRRFPHDMPLELIEITAGKRGKNADIARILQKEGEAMLAAVPKGNRIITLDIPGKKW
DTPQLAEQLESWKLDGRDVSILIGGPEGLAPACKAAADQSWSLSALTLPHPLVRVIMAESLYRAWSVTANHPYHRE

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA [H]

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family [H]

Homologues:

Organism=Escherichia coli, GI1786855, Length=156, Percent_Identity=82.051282051282, Blast_Score=266, Evalue=6e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016051
- InterPro:   IPR003742 [H]

Pfam domain/function: PF02590 SPOUT_MTase [H]

EC number: NA

Molecular weight: Translated: 17434; Mature: 17434

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIQLIAVGTKMPKWVEEGFKEYKRRFPHDMPLELIEITAGKRGKNADIARILQKEGEAM
CEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEE
LAAVPKGNRIITLDIPGKKWDTPQLAEQLESWKLDGRDVSILIGGPEGLAPACKAAADQS
EEECCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCC
WSLSALTLPHPLVRVIMAESLYRAWSVTANHPYHRE
CCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKIQLIAVGTKMPKWVEEGFKEYKRRFPHDMPLELIEITAGKRGKNADIARILQKEGEAM
CEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEE
LAAVPKGNRIITLDIPGKKWDTPQLAEQLESWKLDGRDVSILIGGPEGLAPACKAAADQS
EEECCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCC
WSLSALTLPHPLVRVIMAESLYRAWSVTANHPYHRE
CCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA