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The map label for this gene is lipB

Identifier: 218708729

GI number: 218708729

Start: 743345

End: 744004

Strand: Reverse

Name: lipB

Synonym: VS_0708

Alternate gene names: 218708729

Gene position: 744004-743345 (Counterclockwise)

Preceding gene: 218708730

Following gene: 218708728

Centisome position: 22.55

GC content: 45.61

Gene sequence:

>660_bases
TTGCAAAATAAGCTAATCGTAAAAAAATTAGGTCGTCAGGATTACGAACCTGTATGGAAAGCCATGCATAAGTTCACAGA
CGAACGCACGGATGAAGACGTAGACCAAATTTGGTTGGTTGAACACAACCCAGTTTTCACTCAAGGACAAGCAGGTAAAG
CTGAGCATGTATTAAATGCTGGTGATATCCCTGTGATACAAAGCGATCGCGGTGGCCAAGTGACGTACCACGGGCCCGGT
CAGTTAGTCGCTTACTTCCTGATAAACATCCGCCGCAAGAAATTCGGAGTGCGTGATTTGGTTACTCATATTGAGAACCT
CGTAATCAATACTCTGAAAGCTTACAATATAGATTCAACTGCCCGACCTGACGCTCCTGGTGTTTATGTCGATGGCAAGA
AAATCTGTTCACTCGGATTACGAATTCGACGCGGCTGCTCGTTCCACGGGCTAGCACTCAACGTCGATATGGACTTGTCT
CCATTCCAACGTATTAACCCATGTGGTTACCAAGGGATGGAAATGGCGCAAGTAAGCCAACTCGGCGGTCCAAGTGAACT
AGAAAACGTTGAGCAACAGTTAATACAAGAGCTCGTAGAACTACTCGGCTATGACCAAGTAGACATTCAAGCCACCAGTA
ACATTACAGCAGAAGCATAA

Upstream 100 bases:

>100_bases
ATACAAAGAAAGTTGAAAAACCTTGCTGTCCGTCTGTAGTTAGAAACCTGTTTCGCGTTTATAATGCGCTCACTTTATTA
ATCCTTGAGGAAGTGCCGCT

Downstream 100 bases:

>100_bases
AATCATGAGCAAACCAATCCAAATGGAAAAAGGCGTTAAATATCGTGACGCTGACAAAATGGCATTAATTCCCGTAAAGA
ATATGCCTGCTGAACAGAAA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MQNKLIVKKLGRQDYEPVWKAMHKFTDERTDEDVDQIWLVEHNPVFTQGQAGKAEHVLNAGDIPVIQSDRGGQVTYHGPG
QLVAYFLINIRRKKFGVRDLVTHIENLVINTLKAYNIDSTARPDAPGVYVDGKKICSLGLRIRRGCSFHGLALNVDMDLS
PFQRINPCGYQGMEMAQVSQLGGPSELENVEQQLIQELVELLGYDQVDIQATSNITAEA

Sequences:

>Translated_219_residues
MQNKLIVKKLGRQDYEPVWKAMHKFTDERTDEDVDQIWLVEHNPVFTQGQAGKAEHVLNAGDIPVIQSDRGGQVTYHGPG
QLVAYFLINIRRKKFGVRDLVTHIENLVINTLKAYNIDSTARPDAPGVYVDGKKICSLGLRIRRGCSFHGLALNVDMDLS
PFQRINPCGYQGMEMAQVSQLGGPSELENVEQQLIQELVELLGYDQVDIQATSNITAEA
>Mature_219_residues
MQNKLIVKKLGRQDYEPVWKAMHKFTDERTDEDVDQIWLVEHNPVFTQGQAGKAEHVLNAGDIPVIQSDRGGQVTYHGPG
QLVAYFLINIRRKKFGVRDLVTHIENLVINTLKAYNIDSTARPDAPGVYVDGKKICSLGLRIRRGCSFHGLALNVDMDLS
PFQRINPCGYQGMEMAQVSQLGGPSELENVEQQLIQELVELLGYDQVDIQATSNITAEA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=144, Percent_Identity=35.4166666666667, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI87081767, Length=201, Percent_Identity=65.6716417910448, Blast_Score=285, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6323268, Length=159, Percent_Identity=32.7044025157233, Blast_Score=87, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21355483, Length=147, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_VIBSL (B7VKE7)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416350.1
- GeneID:   7160327
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0708
- HOGENOM:   HBG716160
- OMA:   GDEIWVV
- ProtClustDB:   PRK14342
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 24503; Mature: 24503

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 168-168

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNKLIVKKLGRQDYEPVWKAMHKFTDERTDEDVDQIWLVEHNPVFTQGQAGKAEHVLNA
CCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEECCCCCCHHHHCCC
GDIPVIQSDRGGQVTYHGPGQLVAYFLINIRRKKFGVRDLVTHIENLVINTLKAYNIDST
CCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC
ARPDAPGVYVDGKKICSLGLRIRRGCSFHGLALNVDMDLSPFQRINPCGYQGMEMAQVSQ
CCCCCCCEEECHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHCCCCCCCCCHHHHHHH
LGGPSELENVEQQLIQELVELLGYDQVDIQATSNITAEA
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MQNKLIVKKLGRQDYEPVWKAMHKFTDERTDEDVDQIWLVEHNPVFTQGQAGKAEHVLNA
CCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEECCCCCCHHHHCCC
GDIPVIQSDRGGQVTYHGPGQLVAYFLINIRRKKFGVRDLVTHIENLVINTLKAYNIDST
CCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC
ARPDAPGVYVDGKKICSLGLRIRRGCSFHGLALNVDMDLSPFQRINPCGYQGMEMAQVSQ
CCCCCCCEEECHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHCCCCCCCCCHHHHHHH
LGGPSELENVEQQLIQELVELLGYDQVDIQATSNITAEA
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA