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The map label for this gene is lipA [H]

Identifier: 218708728

GI number: 218708728

Start: 742375

End: 743340

Strand: Reverse

Name: lipA [H]

Synonym: VS_0707

Alternate gene names: 218708728

Gene position: 743340-742375 (Counterclockwise)

Preceding gene: 218708729

Following gene: 218708724

Centisome position: 22.53

GC content: 46.07

Gene sequence:

>966_bases
ATGAGCAAACCAATCCAAATGGAAAAAGGCGTTAAATATCGTGACGCTGACAAAATGGCATTAATTCCCGTAAAGAATAT
GCCTGCTGAACAGAAAGAAGTTTTACGTAAGCCTGCATGGATGAAGATTAAACTTCCTTCAGACAGCCATCGTATTCAAG
AAATCAAATCTGCAATGCGCAAAAACAACCTGCACTCAGTTTGTGAAGAAGCGTCTTGCCCTAACCTAGCTGAGTGTTTT
AACCACGGCACGGCAACGTTTATGATTCTTGGCGCTATCTGTACTCGTCGCTGCCCGTTCTGTGATGTTGCCCATGGTCG
ACCAAATGCTCCAGAAGCCGAAGAGCCGAAGAAACTGGCTAAGACGATTAAAGACATGAAACTGAAGTACGTTGTAATCA
CTTCAGTGGACCGCGATGACTTACGTGATGGCGGTGCTCAACACTTTGCTGATTGTAACCGTGAAATTCGCGAGCAAAAC
CCAAACATTCGTATCGAAACACTGGTTCCGGACTTCCGTGGTCGTATGGACGTTGCGCTTGAACTAATGAAAGACAATCC
ACCAGATGTTTTCAACCACAACCTAGAGACGGCGCCGCGTCTATACCGTAAAGCGCGTCCAGGTGCGAACTACAAGTGGT
CTCTTGATCTGTTAAGAAAATTCAAAGAGCAGCATCCAAACATCCCAACCAAATCAGGTGTGATGATGGGCCTTGGTGAG
ACGAAAGAAGAGATCGTTCAAGTATTGAAAGATCTTCGTGAACATGGCGTAACAATGCTGACACTAGGCCAGTACCTAGC
ACCAAGCCGTCACCACTTGCCAGTTGAACGCTACGTGCCGCCTTCTGAGTTTGATGAGTTGAAAGAGATTGCTCTAGAGC
TAGGCTTCACTCACGCAGCATGTGGTCCGTTTGTACGTTCTTCTTACCATGCAGACTTGCAAGCACAAGGTATGGAAATT
AAGTAA

Upstream 100 bases:

>100_bases
AACGTTGAGCAACAGTTAATACAAGAGCTCGTAGAACTACTCGGCTATGACCAAGTAGACATTCAAGCCACCAGTAACAT
TACAGCAGAAGCATAAAATC

Downstream 100 bases:

>100_bases
TCACGATTGCTTAACGAACAATACAAAGGCGCTGAATTTTATGTTCAGCGCCTTTTTTGTATCGAACAAGCTTGTTCGAT
ACAAAAACAACAAAGCCACT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSKPIQMEKGVKYRDADKMALIPVKNMPAEQKEVLRKPAWMKIKLPSDSHRIQEIKSAMRKNNLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPNAPEAEEPKKLAKTIKDMKLKYVVITSVDRDDLRDGGAQHFADCNREIREQN
PNIRIETLVPDFRGRMDVALELMKDNPPDVFNHNLETAPRLYRKARPGANYKWSLDLLRKFKEQHPNIPTKSGVMMGLGE
TKEEIVQVLKDLREHGVTMLTLGQYLAPSRHHLPVERYVPPSEFDELKEIALELGFTHAACGPFVRSSYHADLQAQGMEI
K

Sequences:

>Translated_321_residues
MSKPIQMEKGVKYRDADKMALIPVKNMPAEQKEVLRKPAWMKIKLPSDSHRIQEIKSAMRKNNLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPNAPEAEEPKKLAKTIKDMKLKYVVITSVDRDDLRDGGAQHFADCNREIREQN
PNIRIETLVPDFRGRMDVALELMKDNPPDVFNHNLETAPRLYRKARPGANYKWSLDLLRKFKEQHPNIPTKSGVMMGLGE
TKEEIVQVLKDLREHGVTMLTLGQYLAPSRHHLPVERYVPPSEFDELKEIALELGFTHAACGPFVRSSYHADLQAQGMEI
K
>Mature_320_residues
SKPIQMEKGVKYRDADKMALIPVKNMPAEQKEVLRKPAWMKIKLPSDSHRIQEIKSAMRKNNLHSVCEEASCPNLAECFN
HGTATFMILGAICTRRCPFCDVAHGRPNAPEAEEPKKLAKTIKDMKLKYVVITSVDRDDLRDGGAQHFADCNREIREQNP
NIRIETLVPDFRGRMDVALELMKDNPPDVFNHNLETAPRLYRKARPGANYKWSLDLLRKFKEQHPNIPTKSGVMMGLGET
KEEIVQVLKDLREHGVTMLTLGQYLAPSRHHLPVERYVPPSEFDELKEIALELGFTHAACGPFVRSSYHADLQAQGMEIK

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=293, Percent_Identity=45.0511945392491, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI37577164, Length=257, Percent_Identity=44.7470817120623, Blast_Score=219, Evalue=4e-57,
Organism=Escherichia coli, GI1786846, Length=321, Percent_Identity=78.816199376947, Blast_Score=548, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI32564533, Length=286, Percent_Identity=42.3076923076923, Blast_Score=226, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=303, Percent_Identity=43.5643564356436, Blast_Score=251, Evalue=8e-68,
Organism=Drosophila melanogaster, GI221513272, Length=289, Percent_Identity=46.0207612456747, Blast_Score=249, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36549; Mature: 36418

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPIQMEKGVKYRDADKMALIPVKNMPAEQKEVLRKPAWMKIKLPSDSHRIQEIKSAMR
CCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHH
KNNLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPNAPEAEEPKKLA
HCHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
KTIKDMKLKYVVITSVDRDDLRDGGAQHFADCNREIREQNPNIRIETLVPDFRGRMDVAL
HHHHHCEEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
ELMKDNPPDVFNHNLETAPRLYRKARPGANYKWSLDLLRKFKEQHPNIPTKSGVMMGLGE
HHHHCCCCHHHCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCCCEEECCCC
TKEEIVQVLKDLREHGVTMLTLGQYLAPSRHHLPVERYVPPSEFDELKEIALELGFTHAA
CHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCHHHH
CGPFVRSSYHADLQAQGMEIK
HHHHHHCCCCCCHHCCCCCCC
>Mature Secondary Structure 
SKPIQMEKGVKYRDADKMALIPVKNMPAEQKEVLRKPAWMKIKLPSDSHRIQEIKSAMR
CCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHH
KNNLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPNAPEAEEPKKLA
HCHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
KTIKDMKLKYVVITSVDRDDLRDGGAQHFADCNREIREQNPNIRIETLVPDFRGRMDVAL
HHHHHCEEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
ELMKDNPPDVFNHNLETAPRLYRKARPGANYKWSLDLLRKFKEQHPNIPTKSGVMMGLGE
HHHHCCCCHHHCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCCCEEECCCC
TKEEIVQVLKDLREHGVTMLTLGQYLAPSRHHLPVERYVPPSEFDELKEIALELGFTHAA
CHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCHHHH
CGPFVRSSYHADLQAQGMEIK
HHHHHHCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]