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The map label for this gene is leuC [H]

Identifier: 218708416

GI number: 218708416

Start: 376895

End: 378307

Strand: Reverse

Name: leuC [H]

Synonym: VS_0378

Alternate gene names: 218708416

Gene position: 378307-376895 (Counterclockwise)

Preceding gene: 218708417

Following gene: 218708415

Centisome position: 11.47

GC content: 46.99

Gene sequence:

>1413_bases
ATGTCGACAAACCAGCAAGCAAAAACCTTATACGAAAAAGTTTATGACGCTCACGTTGCGGTTGCAGCGAAGGGCGAAAC
GCCAATTCTTTATATCGATCGTCACTTAGTCCATGAAGTAACGTCGCCACAAGCCTTTGATGGCCTGCGTGAAAAAGGTC
GTAAAGTTCGCCAAGTAGGCAAAACTTTTGCGACCATGGACCACAACGTATCGACACAAACAAAAGACATCAACGCTTCT
GGTGAGATGGCTCGTATCCAAATGGAAACGCTATCGAAAAACTGTGAAGAGTTTGGTGTCACGCTTTACGACTTAAACCA
TAAATACCAAGGTATTGTTCACGTAATGGGCCCTGAGCTAGGTATTACTCTGCCGGGCATGACCATCGTATGTGGTGACT
CACACACAGCAACACACGGTGCATTTGGCTCATTAGCATTCGGTATCGGTACTTCAGAAGTTGAACATGTTCTAGCAACT
CAAACGCTAAAACAAGCTCGCGCTAAAACCATGAAGATCGAAGTAAAAGGCAAAGTCGCTGAAGGCATTACAGCAAAAGA
TATCGTGCTCGCAATCATCGGTAAGACAACAGCCGCTGGCGGTACAGGCTATGTGGTTGAATTCTGTGGTGAGGCAATTA
CTGACCTTACAATGGAAGGTCGTATGACGGTATGTAACATGGCAATCGAGCTTGGTGCTAAAGCCGGTCTAATTGCTCCA
GATGCAACGACATACGAATACATCAAAGGTCGTAAGTTCTCTCCACAAGGTGAAGACTTAGAAGCGGCAATCGAATACTG
GGACACATTGAAAACTGATGTTGACGCTGAATTCGATGCAGTTGTGACACTAGAAGCAGCAGACATCAAGCCACAAGTGA
CTTGGGGCACTAACCCAGGTCAGGTTATCTCGGTAGACACGCCAATTCCTGCACCAGAAAGCTTTGCAGATCCTGTTGAA
AAAGCTTCAGCAGAAAAGGCTCTGGCGTACATGGGCCTTGAAGCGGGTAAATCACTATCGGATTACAACGTCGATAAAGT
CTTCGTAGGTTCTTGTACTAACTCGCGTATCGAAGACATGCGTGCAGCTGCAGCAGTAGCGAAAGGACGCCAAGTAGCGA
AACATGTTCAAGCATTGATCGTTCCGGGCTCTGAGCAAGTTAAAGCACAAGCTGAAGCTGAAGGCTTAGATGTAATCTTT
AAAGAAGCCGGCTTTGAATGGCGCCTACCAGGCTGCTCTATGTGCCTTGCAATGAACAACGACCGTTTAGGTCCACAAGA
ACGCTGTGCTTCTACATCAAACCGTAACTTTGAAGGTCGCCAAGGCCGTGATGGTCGTACGCACCTAGTTAGCCCAGCAA
TGGCAGCCGCAGCAGCAATCGCTGGTCACTTTGTCGATATTCGTGAACTTTAA

Upstream 100 bases:

>100_bases
GAAGCAAGTACATAAGAATAATATCAAGCCAAGTCAGCTTAAAACGCATGACTTGGCTAGAAACAAACACTGGGACTGAT
GCTCCCAAGGAAGAAAAGCT

Downstream 100 bases:

>100_bases
TTAGAGGATTTAACATGTCAGGTTTTCAACAACACACCGGATTAGTCGTTCCTCTAGATACGGCCAACATCGATACTGAT
GCGATCATTCCAAAGCAGTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSTNQQAKTLYEKVYDAHVAVAAKGETPILYIDRHLVHEVTSPQAFDGLREKGRKVRQVGKTFATMDHNVSTQTKDINAS
GEMARIQMETLSKNCEEFGVTLYDLNHKYQGIVHVMGPELGITLPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLAT
QTLKQARAKTMKIEVKGKVAEGITAKDIVLAIIGKTTAAGGTGYVVEFCGEAITDLTMEGRMTVCNMAIELGAKAGLIAP
DATTYEYIKGRKFSPQGEDLEAAIEYWDTLKTDVDAEFDAVVTLEAADIKPQVTWGTNPGQVISVDTPIPAPESFADPVE
KASAEKALAYMGLEAGKSLSDYNVDKVFVGSCTNSRIEDMRAAAAVAKGRQVAKHVQALIVPGSEQVKAQAEAEGLDVIF
KEAGFEWRLPGCSMCLAMNNDRLGPQERCASTSNRNFEGRQGRDGRTHLVSPAMAAAAAIAGHFVDIREL

Sequences:

>Translated_470_residues
MSTNQQAKTLYEKVYDAHVAVAAKGETPILYIDRHLVHEVTSPQAFDGLREKGRKVRQVGKTFATMDHNVSTQTKDINAS
GEMARIQMETLSKNCEEFGVTLYDLNHKYQGIVHVMGPELGITLPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLAT
QTLKQARAKTMKIEVKGKVAEGITAKDIVLAIIGKTTAAGGTGYVVEFCGEAITDLTMEGRMTVCNMAIELGAKAGLIAP
DATTYEYIKGRKFSPQGEDLEAAIEYWDTLKTDVDAEFDAVVTLEAADIKPQVTWGTNPGQVISVDTPIPAPESFADPVE
KASAEKALAYMGLEAGKSLSDYNVDKVFVGSCTNSRIEDMRAAAAVAKGRQVAKHVQALIVPGSEQVKAQAEAEGLDVIF
KEAGFEWRLPGCSMCLAMNNDRLGPQERCASTSNRNFEGRQGRDGRTHLVSPAMAAAAAIAGHFVDIREL
>Mature_469_residues
STNQQAKTLYEKVYDAHVAVAAKGETPILYIDRHLVHEVTSPQAFDGLREKGRKVRQVGKTFATMDHNVSTQTKDINASG
EMARIQMETLSKNCEEFGVTLYDLNHKYQGIVHVMGPELGITLPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQ
TLKQARAKTMKIEVKGKVAEGITAKDIVLAIIGKTTAAGGTGYVVEFCGEAITDLTMEGRMTVCNMAIELGAKAGLIAPD
ATTYEYIKGRKFSPQGEDLEAAIEYWDTLKTDVDAEFDAVVTLEAADIKPQVTWGTNPGQVISVDTPIPAPESFADPVEK
ASAEKALAYMGLEAGKSLSDYNVDKVFVGSCTNSRIEDMRAAAAVAKGRQVAKHVQALIVPGSEQVKAQAEAEGLDVIFK
EAGFEWRLPGCSMCLAMNNDRLGPQERCASTSNRNFEGRQGRDGRTHLVSPAMAAAAAIAGHFVDIREL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=412, Percent_Identity=27.4271844660194, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI8659555, Length=367, Percent_Identity=26.4305177111717, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI41352693, Length=367, Percent_Identity=26.9754768392371, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=79.9568965517241, Blast_Score=767, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=364, Percent_Identity=27.4725274725275, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081781, Length=363, Percent_Identity=24.5179063360882, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI2367097, Length=350, Percent_Identity=24.5714285714286, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=412, Percent_Identity=28.3980582524272, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI32564738, Length=412, Percent_Identity=28.3980582524272, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI25149342, Length=307, Percent_Identity=30.2931596091205, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17568399, Length=402, Percent_Identity=26.3681592039801, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=59.3617021276596, Blast_Score=567, Evalue=1e-162,
Organism=Saccharomyces cerevisiae, GI6323335, Length=477, Percent_Identity=27.8825995807128, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6322261, Length=405, Percent_Identity=26.9135802469136, Blast_Score=143, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6320440, Length=434, Percent_Identity=25.1152073732719, Blast_Score=127, Evalue=3e-30,
Organism=Drosophila melanogaster, GI281365315, Length=476, Percent_Identity=25.2100840336134, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17864292, Length=476, Percent_Identity=25.2100840336134, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161076999, Length=412, Percent_Identity=25, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI28571643, Length=473, Percent_Identity=24.9471458773784, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24645686, Length=375, Percent_Identity=27.4666666666667, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI17137564, Length=478, Percent_Identity=25.9414225941423, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50474; Mature: 50343

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNQQAKTLYEKVYDAHVAVAAKGETPILYIDRHLVHEVTSPQAFDGLREKGRKVRQVG
CCCCHHHHHHHHHHHHCEEEEEECCCCCEEEECHHHHHHCCCCHHHHHHHHHHHHHHHHH
KTFATMDHNVSTQTKDINASGEMARIQMETLSKNCEEFGVTLYDLNHKYQGIVHVMGPEL
HHHHHCCCCCCCCCCCCCCCCCEEEEEHHHHHCCHHHHCCEEEECCCCCCEEEEEECCCC
GITLPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTLKQARAKTMKIEVKGKVA
CEECCCEEEEECCCCCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHEEEEEEECHHH
EGITAKDIVLAIIGKTTAAGGTGYVVEFCGEAITDLTMEGRMTVCNMAIELGAKAGLIAP
CCCCHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEECC
DATTYEYIKGRKFSPQGEDLEAAIEYWDTLKTDVDAEFDAVVTLEAADIKPQVTWGTNPG
CCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEECCCCC
QVISVDTPIPAPESFADPVEKASAEKALAYMGLEAGKSLSDYNVDKVFVGSCTNSRIEDM
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHH
RAAAAVAKGRQVAKHVQALIVPGSEQVKAQAEAEGLDVIFKEAGFEWRLPGCSMCLAMNN
HHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCEEEEECCCCEEECCCCEEEEEECC
DRLGPQERCASTSNRNFEGRQGRDGRTHLVSPAMAAAAAIAGHFVDIREL
CCCCHHHHHCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHEEHCCC
>Mature Secondary Structure 
STNQQAKTLYEKVYDAHVAVAAKGETPILYIDRHLVHEVTSPQAFDGLREKGRKVRQVG
CCCHHHHHHHHHHHHCEEEEEECCCCCEEEECHHHHHHCCCCHHHHHHHHHHHHHHHHH
KTFATMDHNVSTQTKDINASGEMARIQMETLSKNCEEFGVTLYDLNHKYQGIVHVMGPEL
HHHHHCCCCCCCCCCCCCCCCCEEEEEHHHHHCCHHHHCCEEEECCCCCCEEEEEECCCC
GITLPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTLKQARAKTMKIEVKGKVA
CEECCCEEEEECCCCCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHEEEEEEECHHH
EGITAKDIVLAIIGKTTAAGGTGYVVEFCGEAITDLTMEGRMTVCNMAIELGAKAGLIAP
CCCCHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEECC
DATTYEYIKGRKFSPQGEDLEAAIEYWDTLKTDVDAEFDAVVTLEAADIKPQVTWGTNPG
CCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEECCCCC
QVISVDTPIPAPESFADPVEKASAEKALAYMGLEAGKSLSDYNVDKVFVGSCTNSRIEDM
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHH
RAAAAVAKGRQVAKHVQALIVPGSEQVKAQAEAEGLDVIFKEAGFEWRLPGCSMCLAMNN
HHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCEEEEECCCCEEECCCCEEEEEECC
DRLGPQERCASTSNRNFEGRQGRDGRTHLVSPAMAAAAAIAGHFVDIREL
CCCCHHHHHCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHEEHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA