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The map label for this gene is leuB [H]

Identifier: 218708417

GI number: 218708417

Start: 378425

End: 379516

Strand: Reverse

Name: leuB [H]

Synonym: VS_0379

Alternate gene names: 218708417

Gene position: 379516-378425 (Counterclockwise)

Preceding gene: 218708418

Following gene: 218708416

Centisome position: 11.5

GC content: 47.44

Gene sequence:

>1092_bases
ATGACTGATAAATCATACAAGATTGCCGTACTACCTGGTGATGGCATTGGCCCAGAAGTAATGCAGCAAGCACATAAAGT
GCTAGACGCGATTGAAAAGAAGCACGCGATTAGCTTTTCTCGCGAAGAGTATGATGTTGGTGGTATCGCAATTGATAATC
ACGGCTGTCCACTTCCAGAAGCAACAGTTGCAGGCTGTGAAGAATCTGACGCGGTACTTTTTGGTTCTGTCGGCGGCCCT
AAATGGGAACACCTTCCACCAAACGACCAACCTGAACGTGGTGCCCTACTTCCGCTACGTAAACACTTCCAACTGTTCTG
TAATCTACGTCCTGCACAAATCCATAAAGGTCTAGAGTCTTTCTCTCCTTTGCGTGCAGACATCTCAGGTAACGGCTTCG
ACATCGTTGTTGTTCGTGAACTAACTGGCGGTATCTACTTCGGCCAACCTAAAGGTCGTGAAGGCGAAGGCGCAACAGAG
AAAGCCTTTGATACTGAGGTTTACCACCGTTACGAAATCGAACGAATTGCGAAGATTGCTTTTGAATCCGCTCGTCTACG
TAACAAAAACGTTTACTCAATCGATAAAGCGAACGTTCTACAAAGCTCTATCCTATGGCGTGAAGTGGTTGAAGAAGTAG
CGAAAGACTACCCAGATGTGAAACTAAGCCACATGTACATCGACAACGCGACCATGCAGCTAATCAAAGACCCATCTCAG
TTTGACGTAATGCTATGTTCAAACATCTTTGGTGACATCATCTCTGATGAGTGTGCAATGATCACCGGCTCTATGGGTAT
GCTTCCTTCTGCAAGCTTGAACGAAAGCAACTTCGGCTTATACGAACCTGCGGGCGGCAGTGCTCCAGATATCGCAGGTA
AGAACATCGCGAACCCAGTAGCGCAAATCCTTTCGGCAGCGCTAATGCTGCGTTACAGCCTAGGTGAAGAAGTTGCAGCG
CAAGACATCGAAACAGCGGTATCTAAGGCACTTTCTGCAGGCGAGCTAACTGCAGACCTTGCAGGCAAGAACCAAGCGCT
GACTACCTCAGAAATGGGTGACAAGATCGCTGAGTACATCTTAGCTTCATAA

Upstream 100 bases:

>100_bases
TAATACCCCAATAACAGAGCCCTAGAGCCGATGTTGTTGGCTTTAGGGACAAAATTTAAAACGAGCCAAACAGCTCAGTA
ACAAGAATTAAAGGATTAAC

Downstream 100 bases:

>100_bases
GCTAGATACTTAGATTAGAAGCAAGTACATAAGAATAATATCAAGCCAAGTCAGCTTAAAACGCATGACTTGGCTAGAAA
CAAACACTGGGACTGATGCT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTDKSYKIAVLPGDGIGPEVMQQAHKVLDAIEKKHAISFSREEYDVGGIAIDNHGCPLPEATVAGCEESDAVLFGSVGGP
KWEHLPPNDQPERGALLPLRKHFQLFCNLRPAQIHKGLESFSPLRADISGNGFDIVVVRELTGGIYFGQPKGREGEGATE
KAFDTEVYHRYEIERIAKIAFESARLRNKNVYSIDKANVLQSSILWREVVEEVAKDYPDVKLSHMYIDNATMQLIKDPSQ
FDVMLCSNIFGDIISDECAMITGSMGMLPSASLNESNFGLYEPAGGSAPDIAGKNIANPVAQILSAALMLRYSLGEEVAA
QDIETAVSKALSAGELTADLAGKNQALTTSEMGDKIAEYILAS

Sequences:

>Translated_363_residues
MTDKSYKIAVLPGDGIGPEVMQQAHKVLDAIEKKHAISFSREEYDVGGIAIDNHGCPLPEATVAGCEESDAVLFGSVGGP
KWEHLPPNDQPERGALLPLRKHFQLFCNLRPAQIHKGLESFSPLRADISGNGFDIVVVRELTGGIYFGQPKGREGEGATE
KAFDTEVYHRYEIERIAKIAFESARLRNKNVYSIDKANVLQSSILWREVVEEVAKDYPDVKLSHMYIDNATMQLIKDPSQ
FDVMLCSNIFGDIISDECAMITGSMGMLPSASLNESNFGLYEPAGGSAPDIAGKNIANPVAQILSAALMLRYSLGEEVAA
QDIETAVSKALSAGELTADLAGKNQALTTSEMGDKIAEYILAS
>Mature_362_residues
TDKSYKIAVLPGDGIGPEVMQQAHKVLDAIEKKHAISFSREEYDVGGIAIDNHGCPLPEATVAGCEESDAVLFGSVGGPK
WEHLPPNDQPERGALLPLRKHFQLFCNLRPAQIHKGLESFSPLRADISGNGFDIVVVRELTGGIYFGQPKGREGEGATEK
AFDTEVYHRYEIERIAKIAFESARLRNKNVYSIDKANVLQSSILWREVVEEVAKDYPDVKLSHMYIDNATMQLIKDPSQF
DVMLCSNIFGDIISDECAMITGSMGMLPSASLNESNFGLYEPAGGSAPDIAGKNIANPVAQILSAALMLRYSLGEEVAAQ
DIETAVSKALSAGELTADLAGKNQALTTSEMGDKIAEYILAS

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=353, Percent_Identity=32.8611898016997, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI28178821, Length=314, Percent_Identity=30.8917197452229, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI28178816, Length=314, Percent_Identity=30.8917197452229, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI4758582, Length=365, Percent_Identity=29.5890410958904, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI28178838, Length=337, Percent_Identity=29.3768545994065, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI28178819, Length=151, Percent_Identity=37.7483443708609, Blast_Score=101, Evalue=9e-22,
Organism=Escherichia coli, GI87081683, Length=357, Percent_Identity=75.9103641456583, Blast_Score=569, Evalue=1e-163,
Organism=Escherichia coli, GI1788101, Length=369, Percent_Identity=36.5853658536585, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1787381, Length=400, Percent_Identity=26.25, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71986051, Length=339, Percent_Identity=31.858407079646, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17505779, Length=347, Percent_Identity=31.9884726224784, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17550882, Length=344, Percent_Identity=29.6511627906977, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25144293, Length=361, Percent_Identity=27.9778393351801, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6319830, Length=365, Percent_Identity=46.3013698630137, Blast_Score=284, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6322097, Length=353, Percent_Identity=36.2606232294618, Blast_Score=173, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6324709, Length=344, Percent_Identity=31.6860465116279, Blast_Score=142, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6324291, Length=359, Percent_Identity=30.08356545961, Blast_Score=133, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24643268, Length=340, Percent_Identity=32.0588235294118, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24643270, Length=340, Percent_Identity=32.0588235294118, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24661184, Length=345, Percent_Identity=31.0144927536232, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI161078635, Length=359, Percent_Identity=28.9693593314763, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI161078633, Length=359, Percent_Identity=28.9693593314763, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24650122, Length=359, Percent_Identity=28.9693593314763, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI161078637, Length=359, Percent_Identity=28.9693593314763, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI161078639, Length=348, Percent_Identity=29.5977011494253, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281362242, Length=348, Percent_Identity=26.7241379310345, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648872, Length=348, Percent_Identity=26.7241379310345, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI20130355, Length=349, Percent_Identity=24.0687679083095, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39337; Mature: 39206

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKSYKIAVLPGDGIGPEVMQQAHKVLDAIEKKHAISFSREEYDVGGIAIDNHGCPLPE
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCC
ATVAGCEESDAVLFGSVGGPKWEHLPPNDQPERGALLPLRKHFQLFCNLRPAQIHKGLES
HHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHH
FSPLRADISGNGFDIVVVRELTGGIYFGQPKGREGEGATEKAFDTEVYHRYEIERIAKIA
CCCCEECCCCCCEEEEEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FESARLRNKNVYSIDKANVLQSSILWREVVEEVAKDYPDVKLSHMYIDNATMQLIKDPSQ
HHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHCCCCH
FDVMLCSNIFGDIISDECAMITGSMGMLPSASLNESNFGLYEPAGGSAPDIAGKNIANPV
HHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
AQILSAALMLRYSLGEEVAAQDIETAVSKALSAGELTADLAGKNQALTTSEMGDKIAEYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEHHHHHHHHHHHH
LAS
HCC
>Mature Secondary Structure 
TDKSYKIAVLPGDGIGPEVMQQAHKVLDAIEKKHAISFSREEYDVGGIAIDNHGCPLPE
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCC
ATVAGCEESDAVLFGSVGGPKWEHLPPNDQPERGALLPLRKHFQLFCNLRPAQIHKGLES
HHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHH
FSPLRADISGNGFDIVVVRELTGGIYFGQPKGREGEGATEKAFDTEVYHRYEIERIAKIA
CCCCEECCCCCCEEEEEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FESARLRNKNVYSIDKANVLQSSILWREVVEEVAKDYPDVKLSHMYIDNATMQLIKDPSQ
HHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHCCCCH
FDVMLCSNIFGDIISDECAMITGSMGMLPSASLNESNFGLYEPAGGSAPDIAGKNIANPV
HHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
AQILSAALMLRYSLGEEVAAQDIETAVSKALSAGELTADLAGKNQALTTSEMGDKIAEYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEHHHHHHHHHHHH
LAS
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA