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The map label for this gene is leuD [H]

Identifier: 218708415

GI number: 218708415

Start: 376278

End: 376880

Strand: Reverse

Name: leuD [H]

Synonym: VS_0377

Alternate gene names: 218708415

Gene position: 376880-376278 (Counterclockwise)

Preceding gene: 218708416

Following gene: 218708414

Centisome position: 11.42

GC content: 45.11

Gene sequence:

>603_bases
ATGTCAGGTTTTCAACAACACACCGGATTAGTCGTTCCTCTAGATACGGCCAACATCGATACTGATGCGATCATTCCAAA
GCAGTTTCTACAGAAAGTGAACCGTATCGGTTTTGGTAAGCACTTGTTCCACGATTGGCGTTTTCTAGATGACGCTGGCC
AGCAGCCAAATCCTGAGTTTGTAATGAATGCACCACGCTATAAAGGCGCTTCAATTCTTCTAGCTCGTGAAAACTTTGGT
TGTGGTTCATCTCGTGAACACGCACCCTGGGCGCTTGCCGATTACGGTATTCAAGTGATGATCGCGCCAAGTTTTGCCGA
TATTTTCTACGGTAACTCGATCAACAACCAGATGGTGCCAGTTCGATTGACTGAGCAAGAAGTGGATGAGCTATTCCAAT
TCGTTGAAGCGAATGAAGGTGCAGAAATTACGGTTGACCTAGAAGCAATGAAAGTCCGAGCAAACGGTAAAGAGTACTCG
TTTGAAATTGATGAGTTCCGTCGTCACTGTTTGCTGAATGGCCTAGATAACATCGGCCTAACGCTTCAACATGCCGATAA
GATCTCAGAGTTTGAAGCTAAGATTCCTAGCTTCCTGAAGTAA

Upstream 100 bases:

>100_bases
GTGATGGTCGTACGCACCTAGTTAGCCCAGCAATGGCAGCCGCAGCAGCAATCGCTGGTCACTTTGTCGATATTCGTGAA
CTTTAATTAGAGGATTTAAC

Downstream 100 bases:

>100_bases
AGAAGTCTTGAAATAGAATCACACAGTTTTGTGTTCATATAAAAGGTTGGCTTAGCGCCAACCTTTTTTATTTGGCTAAG
CTTTTAACTTAACTTCATAT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MSGFQQHTGLVVPLDTANIDTDAIIPKQFLQKVNRIGFGKHLFHDWRFLDDAGQQPNPEFVMNAPRYKGASILLARENFG
CGSSREHAPWALADYGIQVMIAPSFADIFYGNSINNQMVPVRLTEQEVDELFQFVEANEGAEITVDLEAMKVRANGKEYS
FEIDEFRRHCLLNGLDNIGLTLQHADKISEFEAKIPSFLK

Sequences:

>Translated_200_residues
MSGFQQHTGLVVPLDTANIDTDAIIPKQFLQKVNRIGFGKHLFHDWRFLDDAGQQPNPEFVMNAPRYKGASILLARENFG
CGSSREHAPWALADYGIQVMIAPSFADIFYGNSINNQMVPVRLTEQEVDELFQFVEANEGAEITVDLEAMKVRANGKEYS
FEIDEFRRHCLLNGLDNIGLTLQHADKISEFEAKIPSFLK
>Mature_199_residues
SGFQQHTGLVVPLDTANIDTDAIIPKQFLQKVNRIGFGKHLFHDWRFLDDAGQQPNPEFVMNAPRYKGASILLARENFGC
GSSREHAPWALADYGIQVMIAPSFADIFYGNSINNQMVPVRLTEQEVDELFQFVEANEGAEITVDLEAMKVRANGKEYSF
EIDEFRRHCLLNGLDNIGLTLQHADKISEFEAKIPSFLK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=196, Percent_Identity=72.4489795918367, Blast_Score=311, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6321429, Length=199, Percent_Identity=50.251256281407, Blast_Score=195, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22577; Mature: 22446

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFQQHTGLVVPLDTANIDTDAIIPKQFLQKVNRIGFGKHLFHDWRFLDDAGQQPNPEF
CCCCCHHCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCE
VMNAPRYKGASILLARENFGCGSSREHAPWALADYGIQVMIAPSFADIFYGNSINNQMVP
EECCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCEEEEEECCHHHHHCCCCCCCCEEE
VRLTEQEVDELFQFVEANEGAEITVDLEAMKVRANGKEYSFEIDEFRRHCLLNGLDNIGL
EEECHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCEEEEEHHHHHHHHHHCCCCCCCE
TLQHADKISEFEAKIPSFLK
EEEHHHHHHHHHHHCHHHCC
>Mature Secondary Structure 
SGFQQHTGLVVPLDTANIDTDAIIPKQFLQKVNRIGFGKHLFHDWRFLDDAGQQPNPEF
CCCCHHCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCE
VMNAPRYKGASILLARENFGCGSSREHAPWALADYGIQVMIAPSFADIFYGNSINNQMVP
EECCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCEEEEEECCHHHHHCCCCCCCCEEE
VRLTEQEVDELFQFVEANEGAEITVDLEAMKVRANGKEYSFEIDEFRRHCLLNGLDNIGL
EEECHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCEEEEEHHHHHHHHHHCCCCCCCE
TLQHADKISEFEAKIPSFLK
EEEHHHHHHHHHHHCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA