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The map label for this gene is frmB [H]

Identifier: 218703642

GI number: 218703642

Start: 435395

End: 436228

Strand: Reverse

Name: frmB [H]

Synonym: ECUMN_0398

Alternate gene names: 218703642

Gene position: 436228-435395 (Counterclockwise)

Preceding gene: 218703643

Following gene: 218703633

Centisome position: 8.39

GC content: 47.96

Gene sequence:

>834_bases
ATGGAACTCATTGAAAAACATGCCAGCTTTGGCGGCTGGCAAAATGTGTATCGGCATTATTCCCCATCACTGAAATGTGA
AATGAATGTCGGCGTCTATCTCCCACCAAAAGCCGCAAGTGAAAAATTGCCGGTGTTGTACTGGCTTTCAGGGCTGACTT
GCAACGAGCAGAATTTCATTACTAAATCGGGGATGCAACGTTACGCGGCTGAGCACAACATTATTGTTGTTGCGCCGGAC
ACCAGTCCGCGAGGCAGTCATGTCGCAGATGCTGACCGTTACGATCTCGGGCAAGGTGCCGGGTTTTACCTGAACGCAAC
GCAAGCGCCGTGGAATGAACATTACAAAATGTATGACTATATCCGCAACGAGCTGCCGGATTTAGTGATGCAACATTTTC
CGGCAACGGCCAGAAAGTCTATCTCAGGTCATTCTATGGGCGGGCTGGGCGCACTGGTGCTGGCGTTACGTAATCCAGAT
GAATATGTCAGCGTCTCAGCGTTTTCGCCCATTGTCTCCCCATCGCAAGTACCGTGGGGACAGCAAGCCTTTACCGCATA
TCTTGGTGAAAATAAAGAAGCCTGGTTGGATTACGACCCGGTGAGCCTTATTTCACAAGGTCAACGCGTTGCGGAAATCA
TGGTTGATCAGGGGTTGAGTGATGATTTTTACGCAGAACAGCTGCGGACTCCAAATCTTGAAAAGATCTGCCAGGAGATG
AATATCAAGACGTTAATCCGTTATCACGAGGGTTATGATCACAGCTATTATTTTGTCTCCAGTTTTATTGGCGAGCATAT
TGCCTATCACGCCAATAAACTGAATATGCGTTAA

Upstream 100 bases:

>100_bases
CGTTACTGATTTCCCGCAGGTTTACCCGTCCAGTTCAGACGGGGTTCTTAATACTCTCCCCGGGCAGCCGTCCGGGGGAT
TAACCGTGAGATAATGACTG

Downstream 100 bases:

>100_bases
TAATAGTGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGCCTACATTTCGCCCGCAGGCCGTAGGCATGATAAGAC
GCGCATAGCGTCGCATCAGG

Product: S-formylglutathione hydrolase

Products: NA

Alternate protein names: FGH [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MELIEKHASFGGWQNVYRHYSPSLKCEMNVGVYLPPKAASEKLPVLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPD
TSPRGSHVADADRYDLGQGAGFYLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATARKSISGHSMGGLGALVLALRNPD
EYVSVSAFSPIVSPSQVPWGQQAFTAYLGENKEAWLDYDPVSLISQGQRVAEIMVDQGLSDDFYAEQLRTPNLEKICQEM
NIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNMR

Sequences:

>Translated_277_residues
MELIEKHASFGGWQNVYRHYSPSLKCEMNVGVYLPPKAASEKLPVLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPD
TSPRGSHVADADRYDLGQGAGFYLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATARKSISGHSMGGLGALVLALRNPD
EYVSVSAFSPIVSPSQVPWGQQAFTAYLGENKEAWLDYDPVSLISQGQRVAEIMVDQGLSDDFYAEQLRTPNLEKICQEM
NIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNMR
>Mature_277_residues
MELIEKHASFGGWQNVYRHYSPSLKCEMNVGVYLPPKAASEKLPVLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPD
TSPRGSHVADADRYDLGQGAGFYLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATARKSISGHSMGGLGALVLALRNPD
EYVSVSAFSPIVSPSQVPWGQQAFTAYLGENKEAWLDYDPVSLISQGQRVAEIMVDQGLSDDFYAEQLRTPNLEKICQEM
NIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNMR

Specific function: Serine hydrolase involved in the detoxification of formaldehyde. Hydrolyzes S-formylglutathione to glutathione and formate [H]

COG id: COG0627

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the esterase D family [H]

Homologues:

Organism=Homo sapiens, GI33413400, Length=280, Percent_Identity=49.6428571428571, Blast_Score=289, Evalue=2e-78,
Organism=Escherichia coli, GI1786551, Length=277, Percent_Identity=96.7509025270758, Blast_Score=566, Evalue=1e-163,
Organism=Escherichia coli, GI1788477, Length=276, Percent_Identity=54.3478260869565, Blast_Score=314, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI17510185, Length=280, Percent_Identity=46.7857142857143, Blast_Score=254, Evalue=4e-68,
Organism=Saccharomyces cerevisiae, GI6322393, Length=298, Percent_Identity=40.9395973154362, Blast_Score=214, Evalue=1e-56,
Organism=Drosophila melanogaster, GI45551932, Length=281, Percent_Identity=49.8220640569395, Blast_Score=283, Evalue=9e-77,
Organism=Drosophila melanogaster, GI24648347, Length=281, Percent_Identity=49.8220640569395, Blast_Score=282, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000801
- InterPro:   IPR014186 [H]

Pfam domain/function: PF00756 Esterase [H]

EC number: =3.1.2.12 [H]

Molecular weight: Translated: 31356; Mature: 31356

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELIEKHASFGGWQNVYRHYSPSLKCEMNVGVYLPPKAASEKLPVLYWLSGLTCNEQNFI
CCHHHHHHCCCCHHHHHHHCCCCEEEEECCCEEECCCCCCCCCCEEEEECCCEECCCCCH
TKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGFYLNATQAPWNEHYKMYDY
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
IRNELPDLVMQHFPATARKSISGHSMGGLGALVLALRNPDEYVSVSAFSPIVSPSQVPWG
HHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHEEEEECCCCCEEEEECCCCCCCCCCCCCC
QQAFTAYLGENKEAWLDYDPVSLISQGQRVAEIMVDQGLSDDFYAEQLRTPNLEKICQEM
HHHHHHHHCCCCCCEECCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHC
NIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNMR
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MELIEKHASFGGWQNVYRHYSPSLKCEMNVGVYLPPKAASEKLPVLYWLSGLTCNEQNFI
CCHHHHHHCCCCHHHHHHHCCCCEEEEECCCEEECCCCCCCCCCEEEEECCCEECCCCCH
TKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGFYLNATQAPWNEHYKMYDY
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
IRNELPDLVMQHFPATARKSISGHSMGGLGALVLALRNPDEYVSVSAFSPIVSPSQVPWG
HHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHEEEEECCCCCEEEEECCCCCCCCCCCCCC
QQAFTAYLGENKEAWLDYDPVSLISQGQRVAEIMVDQGLSDDFYAEQLRTPNLEKICQEM
HHHHHHHHCCCCCCEECCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHC
NIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNMR
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA