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The map label for this gene is frmA

Identifier: 218703643

GI number: 218703643

Start: 436320

End: 437429

Strand: Reverse

Name: frmA

Synonym: ECUMN_0399

Alternate gene names: 218703643

Gene position: 437429-436320 (Counterclockwise)

Preceding gene: 218703644

Following gene: 218703642

Centisome position: 8.41

GC content: 53.69

Gene sequence:

>1110_bases
ATGAAATCACGTGCTGCCGTTGCATTTGCTCCCGGTAAACCGCTGGAAATCGTTGAAATTGACGTTGCACCACCGAAAAA
AGGTGAAGTGCTGATTAAAGTCACCCATACCGGCGTTTGCCATACCGACGCATTTACCCTCTCCGGCGATGACCCGGAAG
GTGTATTCCCGGTAGTTCTCGGTCACGAAGGGGCCGGCGTTGTGGTTGAAGTCGGTGAAGGCGTAACCAGCGTCAAACCT
GGCGACCATGTGATCCCGCTTTACACCGCAGAGTGCGGCGAGTGTGAGTTCTGTCGTTCCGGCAAAACCAACCTCTGTGT
TGCGGTTCGCGAAACCCAGGGTAAAGGCCTGATGCCAGACGGCACCACCCGTTTTTCTTACAACGGGCAGCCGCTTTATC
ACTACATGGGATGCTCTACATTCAGTGAATACACCGTGGTCGCGGAAGTGTCTCTGGCCAAAATTAATCCAGAAGCAAAC
CATGAACACGTCTGCCTGCTGGGCTGTGGCGTGACCACCGGTATTGGCGCGGTACACAACACGGCTAAAGTCCAGCCAGG
TGATTCTGTTGCCGTATTTGGTCTTGGCGCGATTGGTCTGGCAGTGGTTCAGGGCGCGCGTCAGGCGAAAGCGGGACGGA
TTATCGCTATTGATACGAACCCGAAGAAATTCGATCTGGCGCGTCGCTTCGGTGCCACCGACTGCATTAACCCGAATGAC
TACGACAAACCGATTAAAGATGTCCTGCTGGATATCAACAAATGGGGTATCGACCATACCTTTGAATGCATCGGTAACGT
CAACGTGATGCGTGCGGCGCTGGAAAGTGCGCACCGCGGCTGGGGTCAGTCGGTGATCATCGGGGTAGCTGGTTCTGGTC
AGGAAATCTCCACCCGTCCATTCCAGTTGGTCACCGGTCGCGTATGGAAAGGTTCCGCGTTTGGCGGCGTGAAAGGTCGT
TCCCAGTTACCGGGTATGGTTGAAGATGCGATGAAAGGCGATATCGATCTGGAACCGTTTGTCACGCATACCATGAGCCT
GGATGAAATTAATGACGCCTTTGACCTGATGCATGAAGGCAAATCCATTCGAACCGTAATTCGTTACTGA

Upstream 100 bases:

>100_bases
TACAGCCGCGAAGTCAGCCAATCCGTTGACGACACTATTGAACTGGTTCGTGCCTATCTTAAATAGCTGAATCTATTACC
ATATTGAGGAAGAGCGAGAG

Downstream 100 bases:

>100_bases
TTTCCCGCAGGTTTACCCGTCCAGTTCAGACGGGGTTCTTAATACTCTCCCCGGGCAGCCGTCCGGGGGATTAACCGTGA
GATAATGACTGATGGAACTC

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDAFTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKP
GDHVIPLYTAECGECEFCRSGKTNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAIDTNPKKFDLARRFGATDCINPND
YDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALESAHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGR
SQLPGMVEDAMKGDIDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY

Sequences:

>Translated_369_residues
MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDAFTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKP
GDHVIPLYTAECGECEFCRSGKTNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAIDTNPKKFDLARRFGATDCINPND
YDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALESAHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGR
SQLPGMVEDAMKGDIDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY
>Mature_369_residues
MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDAFTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKP
GDHVIPLYTAECGECEFCRSGKTNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAIDTNPKKFDLARRFGATDCINPND
YDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALESAHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGR
SQLPGMVEDAMKGDIDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY

Specific function: Has high formaldehyde dehydrogenase activity in the presence of glutathione and catalyzes the oxidation of normal alcohols in a reaction that is not GSH-dependent

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily

Homologues:

Organism=Homo sapiens, GI71565154, Length=368, Percent_Identity=62.7717391304348, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI34577061, Length=371, Percent_Identity=47.7088948787062, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI71565152, Length=373, Percent_Identity=47.4530831099196, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI4501933, Length=371, Percent_Identity=47.1698113207547, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI156523966, Length=367, Percent_Identity=46.5940054495913, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI4501929, Length=371, Percent_Identity=46.6307277628032, Blast_Score=359, Evalue=2e-99,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=46.814404432133, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI262073058, Length=369, Percent_Identity=46.3414634146341, Blast_Score=351, Evalue=7e-97,
Organism=Homo sapiens, GI71743840, Length=369, Percent_Identity=46.3414634146341, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI156627571, Length=371, Percent_Identity=25.0673854447439, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=99.4579945799458, Blast_Score=754, Evalue=0.0,
Organism=Escherichia coli, GI1790045, Length=358, Percent_Identity=27.9329608938547, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1788073, Length=367, Percent_Identity=28.0653950953678, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI87082125, Length=386, Percent_Identity=26.1658031088083, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1787863, Length=352, Percent_Identity=28.125, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87081918, Length=220, Percent_Identity=35.4545454545455, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786825, Length=246, Percent_Identity=28.0487804878049, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788075, Length=356, Percent_Identity=24.438202247191, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786518, Length=371, Percent_Identity=26.1455525606469, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI226510992, Length=241, Percent_Identity=27.8008298755187, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790718, Length=340, Percent_Identity=24.4117647058824, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788407, Length=231, Percent_Identity=25.974025974026, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=57.372654155496, Blast_Score=457, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=57.0666666666667, Blast_Score=452, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17565904, Length=343, Percent_Identity=56.5597667638484, Blast_Score=419, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17562584, Length=372, Percent_Identity=26.0752688172043, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71988145, Length=219, Percent_Identity=31.9634703196347, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17562582, Length=223, Percent_Identity=29.5964125560538, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320033, Length=372, Percent_Identity=60.4838709677419, Blast_Score=466, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6319621, Length=281, Percent_Identity=31.6725978647687, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323729, Length=368, Percent_Identity=30.1630434782609, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324486, Length=348, Percent_Identity=30.4597701149425, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323961, Length=311, Percent_Identity=30.5466237942122, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323099, Length=377, Percent_Identity=28.1167108753316, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6322619, Length=357, Percent_Identity=27.4509803921569, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319955, Length=357, Percent_Identity=27.4509803921569, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI17737895, Length=368, Percent_Identity=63.5869565217391, Blast_Score=488, Evalue=1e-138,
Organism=Drosophila melanogaster, GI17137530, Length=339, Percent_Identity=24.7787610619469, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI17737897, Length=211, Percent_Identity=27.9620853080569, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FRMA_ECO24 (A7ZIA4)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001461533.1
- ProteinModelPortal:   A7ZIA4
- SMR:   A7ZIA4
- STRING:   A7ZIA4
- EnsemblBacteria:   EBESCT00000023126
- GeneID:   5590872
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_0381
- eggNOG:   COG1062
- GeneTree:   EBGT00050000008796
- HOGENOM:   HBG753318
- OMA:   VDHTFEC
- ProtClustDB:   CLSK2393163
- BioCyc:   ECOL331111:ECE24377A_0381-MONOMER
- GO:   GO:0005737
- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11695
- TIGRFAMs:   TIGR02818

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N; SSF50129 GroES_like

EC number: =1.1.1.284; =1.1.1.1

Molecular weight: Translated: 39333; Mature: 39333

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDAFTLSGDDPEGVFPVVL
CCCCEEEEECCCCCEEEEEEECCCCCCCCEEEEEECCCEEECCEEEECCCCCCCEEEEEE
GHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGKTNLCVAVRETQGKGLMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHCCCCEEEEEEEECCCCCCCCC
GTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEANHEHVCLLGCGVTTGIGAVHN
CCEEEEECCCEEEEEECCCCCCCEEEEEEEEEEEECCCCCCCEEEEEECCCHHCCCHHCC
TAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAIDTNPKKFDLARRFGATDCINPND
CCEECCCCCEEEEEHHHHHHHHHHCHHHHCCCEEEEECCCCCHHHHHHHCCCCCCCCCCC
YDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALESAHRGWGQSVIIGVAGSGQEISTRP
CCCHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
FQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGDIDLEPFVTHTMSLDEINDAFDLMHEG
EEEEECEEECCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHEEEECHHHHHHHHHHHHCC
KSIRTVIRY
CCHHHEECC
>Mature Secondary Structure
MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDAFTLSGDDPEGVFPVVL
CCCCEEEEECCCCCEEEEEEECCCCCCCCEEEEEECCCEEECCEEEECCCCCCCEEEEEE
GHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGKTNLCVAVRETQGKGLMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHCCCCEEEEEEEECCCCCCCCC
GTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEANHEHVCLLGCGVTTGIGAVHN
CCEEEEECCCEEEEEECCCCCCCEEEEEEEEEEEECCCCCCCEEEEEECCCHHCCCHHCC
TAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAIDTNPKKFDLARRFGATDCINPND
CCEECCCCCEEEEEHHHHHHHHHHCHHHHCCCEEEEECCCCCHHHHHHHCCCCCCCCCCC
YDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALESAHRGWGQSVIIGVAGSGQEISTRP
CCCHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
FQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGDIDLEPFVTHTMSLDEINDAFDLMHEG
EEEEECEEECCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHEEEECHHHHHHHHHHHHCC
KSIRTVIRY
CCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA