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The map label for this gene is hemF

Identifier: 218700902

GI number: 218700902

Start: 2676887

End: 2677786

Strand: Direct

Name: hemF

Synonym: ECIAI39_2581

Alternate gene names: 218700902

Gene position: 2676887-2677786 (Clockwise)

Preceding gene: 218700901

Following gene: 218700920

Centisome position: 52.16

GC content: 53.89

Gene sequence:

>900_bases
ATGAAACCCGACGCACACCTGGTTAAACAGTTCCTGCTCAACCTTCAGGATACAATTTGTCAACAACTGACCGCCGTCGA
TGGCGCAGAGTTTGTCGAAGATAGCTGGCAACGTGAAGCTGGCGGCGGTGGGCGTAGTCGGGTGTTGCGTAATGGTGGTG
TTTTCGAACAAGCGGGCGTTAACTTTTCTCACGTTCACGGCGACGCCATGCCCGCTTCCGCCACCGCTCATCGCCCGGAA
CTTGCCGGACGCAGTTTCGAGGCGATGGGCGTTTCACTGGTAGTGCATCCGCATAATCCGTATGTCCCCACCAGCCACGC
GAATGTGCGCTTTTTTATTGCCGAAAAACCGGGTGCAGAGCCAGTCTGGTGGTTTGGTGGCGGCTTCGATTTAACCCCTT
TCTATGGTTTTGAAGAAGACGCCATTCACTGGCACCGCACCGCCCGTGACCTGTGCCTGCCATTTGGCGAAGACGTTTAT
CCCCGTTACAAAAAGTGGTGCGACGAATACTTCTACCTCAAACATCGCAACGAACAACGCGGCATTGGCGGCCTGTTCTT
TGATGACCTGAACACGCCAGACTTCGACCACTGTTTTGCCTTTATGCAGGCGGTAGGCAAAGGCTACACCAACGCTTATT
TACCAATTGTAGAGCGACGTAAAACGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTTATATCGTCGCGGTCGTTAT
GTCGAGTTCAATCTGGTCTGGGATCGTGGCACACTGTTTGGCCTGCAAACCGGCGGGCGCACCGAGTCTATCCTGATGTC
AATGCCGCCACTGGTACGCTGGGAGTATGATTATCAGCCAAAAGATGGCAGCCCGGAAGCGGCGTTAAATGAGTTTATTA
AGGTCAGGGATTGGGTGTAA

Upstream 100 bases:

>100_bases
GGCGTTTCGCCAGAAAATCGCCACAGCGATTGCCGAAGGCGTGATCAGTTATTTCCACTGGTTTGACAACCAGAAAGCAC
ACTCGAGAAAGCGGTAAGTG

Downstream 100 bases:

>100_bases
CTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGCAAACAGTTGCTGATAATCCGTGGC
AAATTGCCCAAGATGCCAGA

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKPDAHLVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGGRSRVLRNGGVFEQAGVNFSHVHGDAMPASATAHRPE
LAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTNAYLPIVERRKTMAYGERERNFQLYRRGRY
VEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPKDGSPEAALNEFIKVRDWV

Sequences:

>Translated_299_residues
MKPDAHLVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGGRSRVLRNGGVFEQAGVNFSHVHGDAMPASATAHRPE
LAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTNAYLPIVERRKTMAYGERERNFQLYRRGRY
VEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPKDGSPEAALNEFIKVRDWV
>Mature_299_residues
MKPDAHLVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGGRSRVLRNGGVFEQAGVNFSHVHGDAMPASATAHRPE
LAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTNAYLPIVERRKTMAYGERERNFQLYRRGRY
VEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPKDGSPEAALNEFIKVRDWV

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III [H]

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI41393599, Length=299, Percent_Identity=47.8260869565217, Blast_Score=283, Evalue=1e-76,
Organism=Escherichia coli, GI1788777, Length=299, Percent_Identity=97.6588628762542, Blast_Score=612, Evalue=1e-176,
Organism=Saccharomyces cerevisiae, GI6320249, Length=317, Percent_Identity=43.5331230283912, Blast_Score=254, Evalue=9e-69,
Organism=Drosophila melanogaster, GI17864388, Length=304, Percent_Identity=48.0263157894737, Blast_Score=286, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001260
- InterPro:   IPR018375 [H]

Pfam domain/function: PF01218 Coprogen_oxidas [H]

EC number: =1.3.3.3 [H]

Molecular weight: Translated: 34345; Mature: 34345

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS01021 COPROGEN_OXIDASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPDAHLVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGGRSRVLRNGGVFEQAGV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCHHCCC
NFSHVHGDAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFIAEKPGAE
CEEEECCCCCCCCCCCCCCHHCCCCEEECCEEEEEECCCCCCCCCCCCEEEEEECCCCCC
PVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVYPRYKKWCDEYFYLKHRNEQR
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
GIGGLFFDDLNTPDFDHCFAFMQAVGKGYTNAYLPIVERRKTMAYGERERNFQLYRRGRY
CCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCE
VEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPKDGSPEAALNEFIKVRDWV
EEEEEEECCCEEEEECCCCCCHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPDAHLVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGGRSRVLRNGGVFEQAGV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCHHCCC
NFSHVHGDAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFIAEKPGAE
CEEEECCCCCCCCCCCCCCHHCCCCEEECCEEEEEECCCCCCCCCCCCEEEEEECCCCCC
PVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVYPRYKKWCDEYFYLKHRNEQR
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
GIGGLFFDDLNTPDFDHCFAFMQAVGKGYTNAYLPIVERRKTMAYGERERNFQLYRRGRY
CCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCE
VEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPKDGSPEAALNEFIKVRDWV
EEEEEEECCCEEEEECCCCCCHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA