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The map label for this gene is amiA

Identifier: 218700901

GI number: 218700901

Start: 2676014

End: 2676883

Strand: Direct

Name: amiA

Synonym: ECIAI39_2580

Alternate gene names: 218700901

Gene position: 2676014-2676883 (Clockwise)

Preceding gene: 218700896

Following gene: 218700902

Centisome position: 52.14

GC content: 51.03

Gene sequence:

>870_bases
ATGAGCACTTTTAAACCACTAAAAACACTCACTTCGCGCCGCCAGGTGCTGAAAGCCGGTTTGGCTGCCCTGACGTTGTC
AGGAATGTCGCAAGCCATCGCCAAAGACGAACCTTTAAAAACCAGCAACGGACACAGCAAGCCGAAAGCCAAAAAATCTG
GCGGCAAACGTGTCGTTGTTCTCGATCCAGGCCACGGCGGGATTGATACCGGAGCGATCGGACGCAACGGTTCGAAAGAA
AAACATGTGGTGCTGGCGATTGCTAAAAACGTCCGTTCCATTTTGCGTAATCATGGCATTGATGCGCGTTTAACGCGTTC
TGGCGATACGTTTATCCCACTATACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATG
CCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGCGGTGCTAGTAGCGCAATGGCG
AAATACCTGTCTGAACGCGAAAACCGTGCTGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAGCA
AGTGCTGTTTGATCTGGTGCAAACCGATACTATTAAAAACAGCCTGACGCTCGGCTCGCATATTCTGAAAAAGATTAAGC
CGGTGCATAAACTGCACAGCCGCAACACCGAACAAGCCGCGTTTGTGGTGTTGAAATCGCCGTCGGTTCCGTCGGTGCTG
GTGGAAACCTCGTTTATCACCAACCCGGAAGAAGAGCGGCTGTTAGGCACGGCGGCGTTTCGCCAGAAAATCGCCACAGC
GATTGCCGAAGGCGTGATCAGTTATTTCCACTGGTTTGACAACCAGAAAGCACACTCGAGAAAGCGGTAA

Upstream 100 bases:

>100_bases
ATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACGGTCGTAACGGATTACGCGATACGATATAACATCTGGAACTTT
ATTATTACAACTCAGGCCGT

Downstream 100 bases:

>100_bases
GTGATGAAACCCGACGCACACCTGGTTAAACAGTTCCTGCTCAACCTTCAGGATACAATTTGTCAACAACTGACCGCCGT
CGATGGCGCAGAGTTTGTCG

Product: N-acetylmuramoyl-l-alanine amidase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNGHSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKE
KHVVLAIAKNVRSILRNHGIDARLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSVPSVL
VETSFITNPEEERLLGTAAFRQKIATAIAEGVISYFHWFDNQKAHSRKR

Sequences:

>Translated_289_residues
MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNGHSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKE
KHVVLAIAKNVRSILRNHGIDARLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSVPSVL
VETSFITNPEEERLLGTAAFRQKIATAIAEGVISYFHWFDNQKAHSRKR
>Mature_288_residues
STFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNGHSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEK
HVVLAIAKNVRSILRNHGIDARLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMAK
YLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSVPSVLV
ETSFITNPEEERLLGTAAFRQKIATAIAEGVISYFHWFDNQKAHSRKR

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=289, Percent_Identity=99.3079584775086, Blast_Score=582, Evalue=1e-168,
Organism=Escherichia coli, GI87082163, Length=236, Percent_Identity=47.4576271186441, Blast_Score=222, Evalue=2e-59,
Organism=Escherichia coli, GI1790611, Length=244, Percent_Identity=38.1147540983607, Blast_Score=172, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 31424; Mature: 31293

Theoretical pI: Translated: 10.87; Mature: 10.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNGHSKPKAKKSGGKRVVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCEEEE
LDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDARLTRSGDTFIPLYDRVEI
ECCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEECCCCCEEEHHHHHHH
AHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMAKYLSERENRADEVAGKKATD
HHHCCCEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
KDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSVPSVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEE
VETSFITNPEEERLLGTAAFRQKIATAIAEGVISYFHWFDNQKAHSRKR
EEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCC
>Mature Secondary Structure 
STFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNGHSKPKAKKSGGKRVVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCEEEE
LDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDARLTRSGDTFIPLYDRVEI
ECCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEECCCCCEEEHHHHHHH
AHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMAKYLSERENRADEVAGKKATD
HHHCCCEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
KDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSVPSVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEE
VETSFITNPEEERLLGTAAFRQKIATAIAEGVISYFHWFDNQKAHSRKR
EEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]