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The map label for this gene is pgaD

Identifier: 218700469

GI number: 218700469

Start: 2177772

End: 2178185

Strand: Direct

Name: pgaD

Synonym: ECIAI39_2135

Alternate gene names: 218700469

Gene position: 2177772-2178185 (Clockwise)

Preceding gene: 218700468

Following gene: 218700475

Centisome position: 42.43

GC content: 43.0

Gene sequence:

>414_bases
ATGAACAATCTAATTATTACGACCCGACAATCGCCAGTACGTTTACTGGTTGATTATGTTGCCACAACCATCTTGTGGAC
ATTATTTGCTTTGTTCATATTCTTATTCGCCATTGATCTGCTGACGGGTTATTACTGGCAAAGCGAGGCCAGAAGCCGAC
TTCAGTTCTATTTTTTGCTGGCAGTGGCGAATGCCGTCGTGTTAATCGTCTGGGCATTGTACAATAAGCTGCGTTTTAAA
AAACAGCAGCATCATGCAGCATACCAATATACGCCGCGAGAATATGCAGAGAGCTTAGCAATACCTGATGAGCTTTATCA
GCAACTACAAAAAAGCCACAGGATGAGCGTACACTTCACCAGCCAGGGACAAATAAAAATGGTGGTTTCAGAAAAAACGC
TAGTCCGGGTATAA

Upstream 100 bases:

>100_bases
TGGCAACGACATTGGTATCATTTACACGAGTCATGGTTGATGCCTAAAAAACAACGCGCTCGTTGGGTAAGTCCCGATCG
TGGGATTCTGAGAGGTTAAT

Downstream 100 bases:

>100_bases
ACACCCAAAACAAAGCCCGGTTCGCCCGGGCTCTGCGCCGATAATACACTTAACTGTATGCATGTAGCGTACGTTGGCAA
AGTGCCGAACGTACGCAATC

Product: putative PGA biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 137; Mature: 137

Protein sequence:

>137_residues
MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAIDLLTGYYWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFK
KQQHHAAYQYTPREYAESLAIPDELYQQLQKSHRMSVHFTSQGQIKMVVSEKTLVRV

Sequences:

>Translated_137_residues
MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAIDLLTGYYWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFK
KQQHHAAYQYTPREYAESLAIPDELYQQLQKSHRMSVHFTSQGQIKMVVSEKTLVRV
>Mature_137_residues
MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAIDLLTGYYWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFK
KQQHHAAYQYTPREYAESLAIPDELYQQLQKSHRMSVHFTSQGQIKMVVSEKTLVRV

Specific function: Required for the synthesis of poly-beta-1,6-N-acetyl-D- glucosamine (PGA), a biofilm adhesin polysaccharide. May assist the glycosyltransferase PgaC in the polymerization of PGA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787258, Length=136, Percent_Identity=97.0588235294118, Blast_Score=272, Evalue=7e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 16150; Mature: 16150

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAIDLLTGYYWQSEARSRLQFYFLL
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVANAVVLIVWALYNKLRFKKQQHHAAYQYTPREYAESLAIPDELYQQLQKSHRMSVHFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCEEEEC
SQGQIKMVVSEKTLVRV
CCCCEEEEECCCHHHCC
>Mature Secondary Structure
MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAIDLLTGYYWQSEARSRLQFYFLL
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVANAVVLIVWALYNKLRFKKQQHHAAYQYTPREYAESLAIPDELYQQLQKSHRMSVHFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCEEEEC
SQGQIKMVVSEKTLVRV
CCCCEEEEECCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]