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The map label for this gene is pgaC

Identifier: 218700468

GI number: 218700468

Start: 2176444

End: 2177718

Strand: Direct

Name: pgaC

Synonym: ECIAI39_2134

Alternate gene names: 218700468

Gene position: 2176444-2177718 (Clockwise)

Preceding gene: 218700467

Following gene: 218700469

Centisome position: 42.41

GC content: 46.59

Gene sequence:

>1275_bases
ATGATTAATCGCATCGTATCGTTTTTTATATTATGTCTGGTGTTATGCATCCCCCTATGCGTAGCGTACTTTCACTCTGG
TGAACTGATGATGAGGTTCGTTTTCTTCTGGCCGTTTTTTATGTCCATTATGTGGATTGTTGGCGGCGTCTATTTCTGGG
TCTATCGTGAACGCCACTGGCCGTGGGGAGAAAACGCACCAGCCCCCCAGTTGAAAGATAATCCGTCTATCTCCATTATC
ATTCCCTGTTTTAATGAGGAGAAAAACGTTGAGGAAACCATACACGCCGCTTTAGCACAGCGTTATGAGAACATTGAAGT
TATTGCCGTAAATGACGGTTCAACAGACAAAACCCGTGCCATCCTGGATCGCATGGCTGCACAAATTCCCCACCTGCGGG
TCATTCATCTGGCGCAAAACCAGGGGAAAGCCATTGCGCTTAAAACCGGAGCTGCCGCGGCGAAAAGTGAATATCTGGTG
TGCATTGATGGCGATGCGTTATTAGACCGCGATGCGGCGGCATATATTGTGGAACCGATGTTGTACAACCCGCGTGTGGG
TGCCGTAACCGGTAATCCTCGTATTCGCACACGTTCTACCCTGGTGGGTAAAATTCAGGTTGGCGAGTATTCCTCAATTA
TTGGTTTGATCAAGCGGACCCAGCGTATCTATGGAAACGTATTTACAGTTTCCGGTGTTATTGCCGCATTTCGTCGCAGC
GCCCTGGCAGAAGTGGGTTACTGGAGTGACGATATGATCACCGAAGATATTGATATTAGCTGGAAGCTGCAGTTGAATCA
GTGGACGATTTTTTACGAGCCACGGGCGCTGTGCTGGATATTAATGCCTGAAACGTTAAAAGGGCTGTGGAAACAGCGCC
TGCGCTGGGCTCAGGGCGGTGCAGAAGTATTCCTCAAAAATATGACAAGGCTATGGCGCAAAGAAAACTTTCGAATGTGG
CCACTGTTTTTTGAATACTGTCTGACGACAATATGGGCCTTTACCTGCCTGGTCGGTTTCATTATTTACGCAGTCCAACT
TGCAGGTGTACCGTTAAATATTGAATTGACACATATCGCAGCGACACATACTGCCGGAATATTATTGTGTACCTTATGTT
TACTGCAATTTATTGTCAGTCTGGTGATTGAGAATCGCTATGAGCACAATCTGGCTTCATCGCTTTTCTGGATTATTTGG
TTCCCGGTTATTTTCTGGATGCTAAGCCTGGCAACGACATTGGTATCATTTACACGAGTCATGGTTGATGCCTAA

Upstream 100 bases:

>100_bases
GGAGTGAAAAATTATGGTTATTATCCCGACAATTTTCTGCATAACCAACCTGAAATAGACGTTATTCGTCCTGAGTTTTC
AACAGCCTGGTATCCGAAAA

Downstream 100 bases:

>100_bases
AAAACAACGCGCTCGTTGGGTAAGTCCCGATCGTGGGATTCTGAGAGGTTAATATGAACAATCTAATTATTACGACCCGA
CAATCGCCAGTACGTTTACT

Product: N-glycosyltransferase

Products: NA

Alternate protein names: PGA synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm PGA synthesis protein PgaC; N-acetylglucosaminyltransferase PgaC [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIMWIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISII
IPCFNEEKNVEETIHAALAQRYENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIKRTQRIYGNVFTVSGVIAAFRRS
ALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRALCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMW
PLFFEYCLTTIWAFTCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLVIENRYEHNLASSLFWIIW
FPVIFWMLSLATTLVSFTRVMVDA

Sequences:

>Translated_424_residues
MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIMWIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISII
IPCFNEEKNVEETIHAALAQRYENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIKRTQRIYGNVFTVSGVIAAFRRS
ALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRALCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMW
PLFFEYCLTTIWAFTCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLVIENRYEHNLASSLFWIIW
FPVIFWMLSLATTLVSFTRVMVDA
>Mature_424_residues
MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIMWIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISII
IPCFNEEKNVEETIHAALAQRYENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIKRTQRIYGNVFTVSGVIAAFRRS
ALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRALCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMW
PLFFEYCLTTIWAFTCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLVIENRYEHNLASSLFWIIW
FPVIFWMLSLATTLVSFTRVMVDA

Specific function: Probable N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N- acetylglucosamine to produce the linear homopolymer poly-beta-1,6- N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide [H]

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=422, Percent_Identity=99.2890995260664, Blast_Score=865, Evalue=0.0,
Organism=Escherichia coli, GI1786560, Length=219, Percent_Identity=29.2237442922374, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 48643; Mature: 48643

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIMWIVGGVYFWVYRERHW
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
PWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRYENIEVIAVNDGSTDKTRA
CCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHH
ILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLVCIDGDALLDRDAAAYIVEPM
HHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCHHHCCCCHHHHCCCC
LYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIKRTQRIYGNVFTVSGVIAAFRRS
EECCCEEEEECCCCEEECCCEEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRALCWILMPETLKGLWKQRLRWAQGG
HHHHCCCCCCCCEECCCCEEEEEEEEEEEEEECCCEEEEEECHHHHHHHHHHHHHHHCCH
AEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAFTCLVGFIIYAVQLAGVPLNIELTHIA
HHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEE
ATHTAGILLCTLCLLQFIVSLVIENRYEHNLASSLFWIIWFPVIFWMLSLATTLVSFTRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVDA
HHCC
>Mature Secondary Structure
MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIMWIVGGVYFWVYRERHW
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
PWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRYENIEVIAVNDGSTDKTRA
CCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHH
ILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLVCIDGDALLDRDAAAYIVEPM
HHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCHHHCCCCHHHHCCCC
LYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIKRTQRIYGNVFTVSGVIAAFRRS
EECCCEEEEECCCCEEECCCEEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRALCWILMPETLKGLWKQRLRWAQGG
HHHHCCCCCCCCEECCCCEEEEEEEEEEEEEECCCEEEEEECHHHHHHHHHHHHHHHCCH
AEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAFTCLVGFIIYAVQLAGVPLNIELTHIA
HHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEE
ATHTAGILLCTLCLLQFIVSLVIENRYEHNLASSLFWIIWFPVIFWMLSLATTLVSFTRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVDA
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]