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The map label for this gene is yafJ

Identifier: 218693707

GI number: 218693707

Start: 277033

End: 277800

Strand: Direct

Name: yafJ

Synonym: EC55989_0247

Alternate gene names: 218693707

Gene position: 277033-277800 (Clockwise)

Preceding gene: 218693706

Following gene: 218693711

Centisome position: 5.37

GC content: 53.52

Gene sequence:

>768_bases
ATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGACTTGTACAGCGTGGTGG
TGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTAAAGATCCACAAC
CCAGCTTTAATTCCCCCATCGCCAAACTTGTCCAGGACTACCCGATAAAATCCTGTTCGGTGGTGGCTCATATTCGCCAG
GCTAATCGGGGCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCA
TAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTCGGTGAAACCGATAGCGAAAAAGCCT
TCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCAGTGTTTAAATATATCGCC
TCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTGCTTTCTGACGGGCGCTATGTGATGGCGTATTGCTC
GACTAATTTACACTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGCTGCTGGATCAGGATGTGGAAATCGACTTCA
GCTCGCAGACCACACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATT
ATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGA

Upstream 100 bases:

>100_bases
ACCGTAGGCCGAATAATTCGTTTACGCCGCATCCGACCTGAAAATTCTTAAATCAATCTTCGCCGGGGGCCATGCGCTCC
CGCTGTTGTGGAGGTTACCC

Downstream 100 bases:

>100_bases
TGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTCGGTG
GCTTACGGGTTTGCTCAAAG

Product: putative glutamine amidotransferases class-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGKGCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQ
ANRGEVALENTHPFTRELWGRNWTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTTPNDVVTVIATQPLTGNETWQKI
MPGEWRLFCLGERVV

Sequences:

>Translated_255_residues
MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGKGCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQ
ANRGEVALENTHPFTRELWGRNWTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTTPNDVVTVIATQPLTGNETWQKI
MPGEWRLFCLGERVV
>Mature_255_residues
MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGKGCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQ
ANRGEVALENTHPFTRELWGRNWTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTTPNDVVTVIATQPLTGNETWQKI
MPGEWRLFCLGERVV

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain

Homologues:

Organism=Escherichia coli, GI1786417, Length=255, Percent_Identity=100, Blast_Score=537, Evalue=1e-154,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YAFJ_ECOLI (Q47147)

Other databases:

- EMBL:   D38582
- EMBL:   U70214
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   H64746
- RefSeq:   AP_000878.1
- RefSeq:   NP_414758.1
- ProteinModelPortal:   Q47147
- SMR:   Q47147
- STRING:   Q47147
- EnsemblBacteria:   EBESCT00000004215
- EnsemblBacteria:   EBESCT00000016966
- GeneID:   944906
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0213
- KEGG:   eco:b0223
- EchoBASE:   EB2941
- EcoGene:   EG13147
- eggNOG:   COG0121
- GeneTree:   EBGT00050000011336
- HOGENOM:   HBG284516
- OMA:   CNTPTDI
- ProtClustDB:   CLSK866480
- BioCyc:   EcoCyc:G6107-MONOMER
- Genevestigator:   Q47147
- InterPro:   IPR000583
- InterPro:   IPR017932

Pfam domain/function: PF00310 GATase_2

EC number: NA

Molecular weight: Translated: 28636; Mature: 28636

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS51278 GATASE_TYPE_2

Important sites: ACT_SITE 2-2

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGKGCRTFKDPQPSFNSPI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHH
AKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRNWTYAHNGQLTGYKSLETG
HHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCCEEECCCCCCCCCCCCC
NFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIASLADELRQKGVFNMLLSDGR
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCC
YVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTTPNDVVTVIATQPLTGNETWQKI
EEEEEECCCEEEEEECCCCCHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCCCHHHHHC
MPGEWRLFCLGERVV
CCCCEEEEEECCCCC
>Mature Secondary Structure
MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGKGCRTFKDPQPSFNSPI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHH
AKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRNWTYAHNGQLTGYKSLETG
HHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCCEEECCCCCCCCCCCCC
NFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIASLADELRQKGVFNMLLSDGR
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCC
YVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTTPNDVVTVIATQPLTGNETWQKI
EEEEEECCCEEEEEECCCCCHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCCCHHHHHC
MPGEWRLFCLGERVV
CCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503