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The map label for this gene is yafL

Identifier: 218693711

GI number: 218693711

Start: 279418

End: 280167

Strand: Direct

Name: yafL

Synonym: EC55989_0251

Alternate gene names: 218693711

Gene position: 279418-280167 (Clockwise)

Preceding gene: 218693707

Following gene: 218693715

Centisome position: 5.42

GC content: 52.67

Gene sequence:

>750_bases
ATGTCCTTGCCGTCGATTCCCTCTTTTGTGTTGTCGGGATTACTGTTGCTTTGCTTGCCGTTTTCTTCATTTGCCAGCGC
CACCACATCACATATCACTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAATCGTGCGCGTTTATTAAAACAGTACCAAA
CCCATCTGAAAAAACAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAGAAGGGCGCTACGCCAGCACAACCGCGAA
CAGATAAAACAGCATCCAGAATGGTTTCCTGCGCCGCTCAAGGCGAGTGACAGACGCTGGCAGGCGCTGGCGGAAAACAA
CCATTTCTTAAGCAGCGACCATCTGCATAACATCACCGAAGTAGCGATTCACCGTCTGGAGCAACAGCTTGGCAAGCCTT
ACGTCTGGGGCGGTACGCGGCCCGATCAAGGCTTCGACTGTAGCGGGTTAGTTTTTTATGCCTACAACAAGATCCTTGAG
GCGAAGCTCCCACGCACGGCCAACGAGATGTATCACTACCATCGGGCAACGATTGTGGCGAATAACGACCTGCGCCGGGG
AGATTTGCTATTTTTCCATATCCACAGCCGCGAGATTGCCGACCATATGGGGGTGTATCTGGGCGACGGTCAGTTTATTG
AGTCACCGCGCACCGGGGAAACCATTCGGGTAAGTCGGTTAGCTGAACCTTTCTGGCAGGATCACTTTTTAGGCGCGCGG
CGGATATTGACGGAAGAGACGATTTTGTAA

Upstream 100 bases:

>100_bases
GAAATATATTTATTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGGTACGGGCCGGGATACACTGCAACATCAATGC
ATGATCAGCCATTATTGAGT

Downstream 100 bases:

>100_bases
ATGGGTAATATTGACGAACTGGCAAAAGGTCGGATGCCACGCGGCATCCGACCTGTTTACATCTGGGCTGAAAACTCACG
TTCTAAGACATCAACAATGA

Product: putative exported hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSYAARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNRE
QIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRLAEPFWQDHFLGAR
RILTEETIL

Sequences:

>Translated_249_residues
MSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSYAARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNRE
QIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRLAEPFWQDHFLGAR
RILTEETIL
>Mature_248_residues
SLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSYAARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQ
IKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEA
KLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRLAEPFWQDHFLGARR
ILTEETIL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1786421, Length=249, Percent_Identity=97.5903614457831, Blast_Score=512, Evalue=1e-147,
Organism=Escherichia coli, GI1787944, Length=134, Percent_Identity=51.4925373134328, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1788501, Length=114, Percent_Identity=32.4561403508772, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 28794; Mature: 28663

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSYAARQRMQNRARLLKQYQTHLKKQA
CCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITE
CEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHH
VAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAKLPRTANEMYHYHRATIVA
HHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEE
NNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRLAEPFWQDHFLGAR
CCCCCCCCEEEEEECCHHHHHHCCEEECCCCEECCCCCCCEEEHHHHHHHHHHHHHHHHH
RILTEETIL
HHHHHHCCC
>Mature Secondary Structure 
SLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSYAARQRMQNRARLLKQYQTHLKKQA
CCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITE
CEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHH
VAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAKLPRTANEMYHYHRATIVA
HHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEE
NNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRLAEPFWQDHFLGAR
CCCCCCCCEEEEEECCHHHHHHCCEEECCCCEECCCCCCCEEEHHHHHHHHHHHHHHHHH
RILTEETIL
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]