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The map label for this gene is nanT2

Identifier: 218692465

GI number: 218692465

Start: 4870834

End: 4872342

Strand: Direct

Name: nanT2

Synonym: ECED1_4913

Alternate gene names: 218692465

Gene position: 4870834-4872342 (Clockwise)

Preceding gene: 218692464

Following gene: 218692466

Centisome position: 93.5

GC content: 42.08

Gene sequence:

>1509_bases
GTGTATTATAAATCCGCTATTATTGTGAGGTTTAATATGGACTCCTGTATTCAGAAAAAGCAGGTTTGGTATAAGCATCT
TTCACCCAGACAGTGGAAGATATTCGGTGCGGCATGGACAGGTTATTTACTTGATGGTTTTGATTTCGTTCTGATATCTC
TGGTTCTTACAGAGGTTCAGCATGAATTCGGCTTGACGACAATAGAAGCGGCAAGCCTTATTTCCGCTGCTTTTATTTCC
CGATGGTTTGGGGGGCTTGCTATTGGCGCTTTATCGGACAAGATGGGGCGCAGGATGGCAATGGTTTTAAGTATAGTGCT
ATTCTCTCTCGGAACCCTTGCCTGTGGTCTGGCTCCTGGATATGCAGTGATGTTTATTGCTCGCATTGTTATTGGGCTTG
GTATGGCAGGTGAATATGGCTCAAGTGTAACATATGTTATCGAAAGCTGGCCTGTTCATCTCAGAAACAAGGCAAGTGGT
TTTTTAATTTCTGGATTTTCTATTGGGGGAGGTTTAGCTGCACAGGTTTATAGCATTGTAGTACCACTATGGGGCTGGCG
TTCTCTTTTCTTTGTCGGCATGCTTCCAATATTATTTGCTTTTTATCTTCGCAAGAATCTTCCGGAGTCTGATGACTGGC
AAAAAAGGCAACAAGAGAATAAACCCGTCAGAACAATGGTGGATATTCTGTACAGAGAAAAGAATAAATACATAAATATT
CTTCTGTCTTGTATTGCATTTGCATGTCTATATGTTTGTTTTTCAGGCGTCACTGCAAACGCTGCTTTAATCACAGTTAT
GGCATTGTGCTGTGCGGCTGTTTTTATCAGTTTTATATATCAGGGTATGGGGAAAAGATGGCCAACAGGCATTATGTTGA
TGCTTGTGGTTATGTTCTGTTTCCTCTACGGATGGCCACTGCAGGCTTTTCTTCCTACATGGCTTAAGGTTGATATGCAG
TATTCACCAGAAACTGTTGCTCTCATATTTATGCTGGCAGGTTTTGGCTCTGCAGCTGGAAGTTGTATAGGTGGTTTTAT
GGGGGACTGGCTTGGAACCCGAAAAGCATATGTTATATCACTTCTTATCGGTCAGCTTGTTATTATCCCCGTTTTTCTTG
TGGACCGGGACTATGTATGGTTACTTGGCCTTCTAATCTTTACTCAGCAGGTTTTTGGTCAGGGGATTGGTGCTCTGGTG
CCGAAAATAATCAGTGGTTATTTTAATGTGGAGCAGCGTGCGGCAGGACTGGGGTTTATTTATAATGTAGGTTCACTTGG
TGGAGCATGTGCGCCTATTCTTGGTGCAGTTGTTGCGTCTCATACAAGTCTTGGTACAGCAATGTGTTCTCTTGCTTTTA
TCTTAACATTTGTTGTTCTTGTCCTTATTGGTTTTGATATGCCATCACGTGTACAGAGATGGATCCATCCAGAAGCTGCG
CTTGAATATGATACTGTCGATGGAAAACCTTTTTATGGAGCAAGAAAAAAGAATGTTGCAGAAGAATAA

Upstream 100 bases:

>100_bases
TTGTAATGTAATGCTGTTTCCGGCATATTTCAGAAGTGACTCTGGTCTTATTGGTGCAACTCTTTGGGACCGGGATTGTA
TTACTTAAAGTAATACTCCA

Downstream 100 bases:

>100_bases
CGGATGCTATCAGGGAATAAAAAAATGATTCATTACAGGGAGAATATATATTCTCTTGATAATTATGAGCGTTACATAAC
TGACAGTTCGCTATTTGAGA

Product: putative sialic acid transporter

Products: Proton [Cytoplasm]; N-acetylneuraminate [Cytoplasm] [C]

Alternate protein names: Sialic acid permease 2

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MYYKSAIIVRFNMDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVLTEVQHEFGLTTIEAASLISAAFIS
RWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLACGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASG
FLISGFSIGGGLAAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDILYREKNKYINI
LLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWPTGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQ
YSPETVALIFMLAGFGSAAGSCIGGFMGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALV
PKIISGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVLIGFDMPSRVQRWIHPEAA
LEYDTVDGKPFYGARKKNVAEE

Sequences:

>Translated_502_residues
MYYKSAIIVRFNMDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVLTEVQHEFGLTTIEAASLISAAFIS
RWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLACGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASG
FLISGFSIGGGLAAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDILYREKNKYINI
LLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWPTGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQ
YSPETVALIFMLAGFGSAAGSCIGGFMGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALV
PKIISGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVLIGFDMPSRVQRWIHPEAA
LEYDTVDGKPFYGARKKNVAEE
>Mature_502_residues
MYYKSAIIVRFNMDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVLTEVQHEFGLTTIEAASLISAAFIS
RWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLACGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASG
FLISGFSIGGGLAAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDILYREKNKYINI
LLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWPTGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQ
YSPETVALIFMLAGFGSAAGSCIGGFMGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALV
PKIISGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVLIGFDMPSRVQRWIHPEAA
LEYDTVDGKPFYGARKKNVAEE

Specific function: May be a sugar-cation symporter involved in sialic acid uptake

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Sialic acid transporter (TC 2.A.1.12.1) subfamily

Homologues:

Organism=Escherichia coli, GI87082231, Length=480, Percent_Identity=66.0416666666667, Blast_Score=674, Evalue=0.0,
Organism=Escherichia coli, GI87082404, Length=451, Percent_Identity=32.1507760532151, Blast_Score=265, Evalue=6e-72,
Organism=Escherichia coli, GI1790550, Length=506, Percent_Identity=21.9367588932806, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1788942, Length=241, Percent_Identity=31.1203319502075, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788292, Length=437, Percent_Identity=20.8237986270023, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI87081723, Length=435, Percent_Identity=23.6781609195402, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NANT2_ECOL6 (Q8FDU9)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_755512.2
- ProteinModelPortal:   Q8FDU9
- EnsemblBacteria:   EBESCT00000042041
- GeneID:   1039597
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c3637
- NMPDR:   fig|199310.1.peg.3555
- GeneTree:   EBGT00050000008816
- HOGENOM:   HBG417084
- OMA:   WRGMFFV
- ProtClustDB:   PRK03893
- HAMAP:   MF_01238
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004742
- InterPro:   IPR005829

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 55355; Mature: 55355

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS50850 MFS; PS00216 SUGAR_TRANSPORT_1; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x26183258)-; HASH(0x257ca1dc)-; HASH(0x263a3e54)-; HASH(0x2643fd90)-; HASH(0x263812f4)-; HASH(0x26023bf4)-; HASH(0x25e27924)-; HASH(0x26232cdc)-; HASH(0x25966940)-; HASH(0x23fd6920)-; HASH(0x263783dc)-; HASH(0x2613e018)-; HASH(0x25b5029c)-; HASH(0x260875f0)-;

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYKSAIIVRFNMDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVLTEVQ
CCCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
HEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLACGLAPG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
YAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGLAAQVYSIV
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHCCCCCCEEEECCCCCCHHHHHHHHHH
VPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDILYREKNKYINI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWPTGIMLMLVVMFC
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGFMGDWLGTRKAYVIS
HHHCCCHHHHHHHHHEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHH
LLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKIISGYFNVEQRAAGLGFI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHCCCHHE
YNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVLIGFDMPSRVQRWIHPEAA
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHH
LEYDTVDGKPFYGARKKNVAEE
CEECCCCCCCCCCCCCCCCCCH
>Mature Secondary Structure
MYYKSAIIVRFNMDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVLTEVQ
CCCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
HEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLACGLAPG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
YAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGLAAQVYSIV
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHCCCCCCEEEECCCCCCHHHHHHHHHH
VPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDILYREKNKYINI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWPTGIMLMLVVMFC
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGFMGDWLGTRKAYVIS
HHHCCCHHHHHHHHHEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHH
LLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKIISGYFNVEQRAAGLGFI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHCCCHHE
YNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVLIGFDMPSRVQRWIHPEAA
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHH
LEYDTVDGKPFYGARKKNVAEE
CEECCCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; N-acetylneuraminate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + N-acetylneuraminate [Periplasm] = Proton [Cytoplasm] + N-acetylneuraminate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157