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The map label for this gene is ycbF [H]

Identifier: 218550688

GI number: 218550688

Start: 3463178

End: 3463978

Strand: Reverse

Name: ycbF [H]

Synonym: EFER_3399

Alternate gene names: 218550688

Gene position: 3463978-3463178 (Counterclockwise)

Preceding gene: 218550689

Following gene: 218550687

Centisome position: 75.49

GC content: 39.2

Gene sequence:

>801_bases
ATGATGTACAACCAGGAGATGCGAATGCATCAGTTTCCTTTATCGTCACTTATGATTAATGTGCTGATATGCATTTTAAT
ATGTTGCACAAATGCGCAAGCAGGAGTTGTTGTTGGCGGTACTCGTTTTGTTTACCCAGAAAAGCAGTCATCAATTTCGG
TCGAGCTTAAAAATACTGCCAGTAAAGATATGCTGGTTAAAATCTCCGTGACACCTGATGATGGCCGTCAGTTAAAAGGG
ACGCTGGAATCTCAGCCTTTACTTCCAGATAAGGCATTTATTGTCACACCACCATTATTGGTACTTAAACAAGATAAAAA
TACTAAAATAAGAATTAATGGCATGGGTGCTTCATTACCTGCAGATCGTGAGAGTTTATTTATACTGAATGTGGCAGCGT
TACCTGCTCAGGAACAAAACTCACAGCTTAAGAACGAGAATCAACTTCAGGTAGCTGTACGTAATCGGATGAAAATATTT
TATCGCCCAGCGAAGTTATCAGGTAATGCACTTGAAGCTTATCAACAGTTACGCTGGTCACGAAACAATGAAGTTGTCAC
CATTTTTAACCCGACTCCCTGGTATGTGACACTCTATAATTTATCTATTGATGGTAAAAGCATAAATGGTGGCATGGTCG
CACCATTTACTCATCGTCAACAGAATTGGTGTCAGCGGCAAGGATATTGCGAGATTAGCTGGCAAACATTGGATCTTTAT
AATAATGCCTTACCTGAATGGAACGTAAAGTTAAATGTTTTTCAAAATAATGCAATGGGTGCAGTAGCAATACATGGATA
A

Upstream 100 bases:

>100_bases
AGACAAAGTATTAGTAATGGTATAAAGAGTACAAGTTTTGCCCTTACACCATTGGTAGATAATACATTGCATTTCTTTGC
TGCTTATAAAGCTATAAAAA

Downstream 100 bases:

>100_bases
CTTTGGAGGCATGATGCTTACTTATTCTTGTGGACATATAAAAGAAATTATTTTATTGTTTGTTCTATCGATCTGTAGTT
GCTATAACATCGTATATGCT

Product: periplasmic pilin chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MMYNQEMRMHQFPLSSLMINVLICILICCTNAQAGVVVGGTRFVYPEKQSSISVELKNTASKDMLVKISVTPDDGRQLKG
TLESQPLLPDKAFIVTPPLLVLKQDKNTKIRINGMGASLPADRESLFILNVAALPAQEQNSQLKNENQLQVAVRNRMKIF
YRPAKLSGNALEAYQQLRWSRNNEVVTIFNPTPWYVTLYNLSIDGKSINGGMVAPFTHRQQNWCQRQGYCEISWQTLDLY
NNALPEWNVKLNVFQNNAMGAVAIHG

Sequences:

>Translated_266_residues
MMYNQEMRMHQFPLSSLMINVLICILICCTNAQAGVVVGGTRFVYPEKQSSISVELKNTASKDMLVKISVTPDDGRQLKG
TLESQPLLPDKAFIVTPPLLVLKQDKNTKIRINGMGASLPADRESLFILNVAALPAQEQNSQLKNENQLQVAVRNRMKIF
YRPAKLSGNALEAYQQLRWSRNNEVVTIFNPTPWYVTLYNLSIDGKSINGGMVAPFTHRQQNWCQRQGYCEISWQTLDLY
NNALPEWNVKLNVFQNNAMGAVAIHG
>Mature_266_residues
MMYNQEMRMHQFPLSSLMINVLICILICCTNAQAGVVVGGTRFVYPEKQSSISVELKNTASKDMLVKISVTPDDGRQLKG
TLESQPLLPDKAFIVTPPLLVLKQDKNTKIRINGMGASLPADRESLFILNVAALPAQEQNSQLKNENQLQVAVRNRMKIF
YRPAKLSGNALEAYQQLRWSRNNEVVTIFNPTPWYVTLYNLSIDGKSINGGMVAPFTHRQQNWCQRQGYCEISWQTLDLY
NNALPEWNVKLNVFQNNAMGAVAIHG

Specific function: Could be required for the biogenesis of a putative fimbria [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI87081806, Length=235, Percent_Identity=35.7446808510638, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786743, Length=237, Percent_Identity=31.6455696202532, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787171, Length=236, Percent_Identity=32.2033898305085, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1790771, Length=229, Percent_Identity=32.7510917030568, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1786333, Length=207, Percent_Identity=32.3671497584541, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789532, Length=229, Percent_Identity=27.9475982532751, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1788428, Length=218, Percent_Identity=28.4403669724771, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1788677, Length=181, Percent_Identity=31.4917127071823, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1786936, Length=205, Percent_Identity=28.2926829268293, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 29988; Mature: 29988

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMYNQEMRMHQFPLSSLMINVLICILICCTNAQAGVVVGGTRFVYPEKQSSISVELKNTA
CCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEECCEEEECCCCCCEEEEEECCCC
SKDMLVKISVTPDDGRQLKGTLESQPLLPDKAFIVTPPLLVLKQDKNTKIRINGMGASLP
CCCEEEEEEECCCCCCEEECCCCCCCCCCCCEEEECCCEEEEECCCCCEEEEEECCCCCC
ADRESLFILNVAALPAQEQNSQLKNENQLQVAVRNRMKIFYRPAKLSGNALEAYQQLRWS
CCCCEEEEEEEEECCCCCHHHHCCCCCEEEEEEECCEEEEEECCCCCCHHHHHHHHHHHC
RNNEVVTIFNPTPWYVTLYNLSIDGKSINGGMVAPFTHRQQNWCQRQGYCEISWQTLDLY
CCCCEEEEECCCCCEEEEEEEEECCCCCCCCEECCCCCHHHHHHHHCCEEEEEEEEEEHH
NNALPEWNVKLNVFQNNAMGAVAIHG
HCCCCCCEEEEEEEECCCEEEEEEEC
>Mature Secondary Structure
MMYNQEMRMHQFPLSSLMINVLICILICCTNAQAGVVVGGTRFVYPEKQSSISVELKNTA
CCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEECCEEEECCCCCCEEEEEECCCC
SKDMLVKISVTPDDGRQLKGTLESQPLLPDKAFIVTPPLLVLKQDKNTKIRINGMGASLP
CCCEEEEEEECCCCCCEEECCCCCCCCCCCCEEEECCCEEEEECCCCCEEEEEECCCCCC
ADRESLFILNVAALPAQEQNSQLKNENQLQVAVRNRMKIFYRPAKLSGNALEAYQQLRWS
CCCCEEEEEEEEECCCCCHHHHCCCCCEEEEEEECCEEEEEECCCCCCHHHHHHHHHHHC
RNNEVVTIFNPTPWYVTLYNLSIDGKSINGGMVAPFTHRQQNWCQRQGYCEISWQTLDLY
CCCCEEEEECCCCCEEEEEEEEECCCCCCCCEECCCCCHHHHHHHHCCEEEEEEEEEEHH
NNALPEWNVKLNVFQNNAMGAVAIHG
HCCCCCCEEEEEEEECCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 2992959; 7984428 [H]