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The map label for this gene is pcaG [H]

Identifier: 218548856

GI number: 218548856

Start: 1536269

End: 1536889

Strand: Reverse

Name: pcaG [H]

Synonym: EFER_1501

Alternate gene names: 218548856

Gene position: 1536889-1536269 (Counterclockwise)

Preceding gene: 218548857

Following gene: 218548855

Centisome position: 33.49

GC content: 48.47

Gene sequence:

>621_bases
ATGAAGAATTATTTAGCGGAAACAGCTTCGCAAACTGCCGGGCCATATGTGCACATTGGCCTCGCCCCTGATGCCGCTGG
TTTTCATATTTTTGAGAAGAATTTCAGTTCAACGCTCGTTAACAGCCATACCGAAGGTGAGCGAATTACTATTGAAGGTC
GGGTGATTGATGGTTCAGGCACGCCAGTACGCGATGTGTTGCTGGAGATTTGGCAGGCCAACGCCAATGGCCGTTATAAC
CATCCGGCAGATCAACAAAGTGATAAAAAACTGGATGCTGATTTTCGTGGTTGGGGCCGCAGTTGCTCAGATTTCGACAC
TGGGATCTGGCGCTTCGAAACCATAAAACCAGGGAGTGTTGTCGGTCGCGACGGTCGTACTATGGCGCCACACGTTAACT
TATGGGTCGTGGCGCGAGGTATCAATATAGGCCTGAATACACGGATGTATTTTCCCGATGAGCAAGATGCCAACCAGCAC
GATCCCGTGCTGAATTTGATTGAATGGGAAGTAAGACGCCAGACGCTGATCGGCAAGCGAGAACAGCGTGGCAACGAAAC
AGTTTATATCTTCGATATTCATCTGCAAGGTGACAAAGAAACCGTCTTTTTTGATGTGTAA

Upstream 100 bases:

>100_bases
ACACCGCGATTCCTCTCGACAGCCTGGCATATCGCTTTGATTTGGTATTACGTGGTCAACGTGCCACGCTGTTCGAAAAC
CGCACTCAGGGAGTGGCAAA

Downstream 100 bases:

>100_bases
ATCATTATCGGGTTGAATTCGTTCAACCCGAATTCAACCATTCTCTTCTCTCTTATTGCTGTTCAAAAATCAAGCAACAA
AATATTTGGTAATATAAAAT

Product: protocatechuate 3,4-dioxygenase alpha chain

Products: NA

Alternate protein names: 3,4-PCD [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MKNYLAETASQTAGPYVHIGLAPDAAGFHIFEKNFSSTLVNSHTEGERITIEGRVIDGSGTPVRDVLLEIWQANANGRYN
HPADQQSDKKLDADFRGWGRSCSDFDTGIWRFETIKPGSVVGRDGRTMAPHVNLWVVARGINIGLNTRMYFPDEQDANQH
DPVLNLIEWEVRRQTLIGKREQRGNETVYIFDIHLQGDKETVFFDV

Sequences:

>Translated_206_residues
MKNYLAETASQTAGPYVHIGLAPDAAGFHIFEKNFSSTLVNSHTEGERITIEGRVIDGSGTPVRDVLLEIWQANANGRYN
HPADQQSDKKLDADFRGWGRSCSDFDTGIWRFETIKPGSVVGRDGRTMAPHVNLWVVARGINIGLNTRMYFPDEQDANQH
DPVLNLIEWEVRRQTLIGKREQRGNETVYIFDIHLQGDKETVFFDV
>Mature_206_residues
MKNYLAETASQTAGPYVHIGLAPDAAGFHIFEKNFSSTLVNSHTEGERITIEGRVIDGSGTPVRDVLLEIWQANANGRYN
HPADQQSDKKLDADFRGWGRSCSDFDTGIWRFETIKPGSVVGRDGRTMAPHVNLWVVARGINIGLNTRMYFPDEQDANQH
DPVLNLIEWEVRRQTLIGKREQRGNETVYIFDIHLQGDKETVFFDV

Specific function: Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway [H]

COG id: COG3485

COG function: function code Q; Protocatechuate 3,4-dioxygenase beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the intradiol ring-cleavage dioxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000627
- InterPro:   IPR015889
- InterPro:   IPR012786 [H]

Pfam domain/function: PF00775 Dioxygenase_C [H]

EC number: =1.13.11.3 [H]

Molecular weight: Translated: 23288; Mature: 23288

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00083 INTRADIOL_DIOXYGENAS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNYLAETASQTAGPYVHIGLAPDAAGFHIFEKNFSSTLVNSHTEGERITIEGRVIDGSG
CCCHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCHHHHHCCCCCCCEEEEEEEEECCCC
TPVRDVLLEIWQANANGRYNHPADQQSDKKLDADFRGWGRSCSDFDTGIWRFETIKPGSV
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCEEEEEECCCCE
VGRDGRTMAPHVNLWVVARGINIGLNTRMYFPDEQDANQHDPVLNLIEWEVRRQTLIGKR
ECCCCCEECCCCEEEEEEECCEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCH
EQRGNETVYIFDIHLQGDKETVFFDV
HHCCCCEEEEEEEEEECCCEEEEEEC
>Mature Secondary Structure
MKNYLAETASQTAGPYVHIGLAPDAAGFHIFEKNFSSTLVNSHTEGERITIEGRVIDGSG
CCCHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCHHHHHCCCCCCCEEEEEEEEECCCC
TPVRDVLLEIWQANANGRYNHPADQQSDKKLDADFRGWGRSCSDFDTGIWRFETIKPGSV
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCEEEEEECCCCE
VGRDGRTMAPHVNLWVVARGINIGLNTRMYFPDEQDANQHDPVLNLIEWEVRRQTLIGKR
ECCCCCEECCCCEEEEEEECCEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCH
EQRGNETVYIFDIHLQGDKETVFFDV
HHCCCCEEEEEEEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2298704; 8063101 [H]