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The map label for this gene is fliY [H]

Identifier: 218548543

GI number: 218548543

Start: 1214132

End: 1214932

Strand: Direct

Name: fliY [H]

Synonym: EFER_1174

Alternate gene names: 218548543

Gene position: 1214132-1214932 (Clockwise)

Preceding gene: 218548542

Following gene: 218548544

Centisome position: 26.46

GC content: 48.19

Gene sequence:

>801_bases
ATGAAATTAGCACTTCTGGGACGTCGGGCGTTGTTGAGCGTTATGGCGCTGGCACTGGTTGCCGGAATGAGCGCAAAAAC
CTTTGCAGATGAAGGCCTGTTAAATAAAGTTAAAGAGCGCGGCACGCTGCTGGTGGGCCTCGAAGGTACTTATCCTCCGT
TCAGTTTTCAGGGCGATGATGGCAAACTGACCGGTTTTGAAGTGGAATTTGCCCAGCAACTGGCAAAACATCTCGGTGTT
GAAGCGACGCTTAAACCAACCAAATGGGACGGAATGCTGGCATCGCTGGATTCAAAACGTATTGATGTGGTGATTAATCA
GGTCACCATCTCTGACGAACGCAAGAAAAAATATGACTTCTCTACCCCCTACACCGTCTCCGGTATTCAGGCGCTGGTGA
AAAAAGGTAATGAAGGAACGATTAAAACGGCGAATGATTTAAAAGGTAAAAAAGTGGGTGTCGGATTAGGGACGAACTAT
GAAGAGTGGTTGCGCCAGAATGTGCAGGGCGTAGACATTCGTACCTATGACGATGATCCAACCAAATATCAGGATCTGCG
AGTCGGTCGAATTGATGCCATCCTGGTGGATCGTCTTGCCGCACTGGATTTGGTAAAGAAAACCAAAGACACACTTGCGG
TAACAGGTGACGCGTTCTCCCGTCAGGAATCGGGCGTGGCTGTGCGTAAAAACAATGAAGACCTGCTGAAAGCGGTCAAT
GAAGCGATTGCCGAAATGCAAAAAGATGGCACTTTGCAGGCACTCTCACAAAAATGGTTTGGCGCTGATGTGACGAAATA
A

Upstream 100 bases:

>100_bases
TGATATATATTAAAAATGAATAAGATGTAACGGAGTGTTTTTTGGCGCGAGTCAGAAACGCTCTACACTGATGCCGTTAC
TGACAACATTTGGGGTAATT

Downstream 100 bases:

>100_bases
TCATTGTAAACGACAAAAAAGGCGCTTTAACCGGCGCCTTTTTTATTTAATTCTCAACCGGGTGCATAATAAACAATAAC
TCACATCAATGTTTACCGGA

Product: cystine transporter subunit

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKLALLGRRALLSVMALALVAGMSAKTFADEGLLNKVKERGTLLVGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGV
EATLKPTKWDGMLASLDSKRIDVVINQVTISDERKKKYDFSTPYTVSGIQALVKKGNEGTIKTANDLKGKKVGVGLGTNY
EEWLRQNVQGVDIRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTKDTLAVTGDAFSRQESGVAVRKNNEDLLKAVN
EAIAEMQKDGTLQALSQKWFGADVTK

Sequences:

>Translated_266_residues
MKLALLGRRALLSVMALALVAGMSAKTFADEGLLNKVKERGTLLVGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGV
EATLKPTKWDGMLASLDSKRIDVVINQVTISDERKKKYDFSTPYTVSGIQALVKKGNEGTIKTANDLKGKKVGVGLGTNY
EEWLRQNVQGVDIRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTKDTLAVTGDAFSRQESGVAVRKNNEDLLKAVN
EAIAEMQKDGTLQALSQKWFGADVTK
>Mature_266_residues
MKLALLGRRALLSVMALALVAGMSAKTFADEGLLNKVKERGTLLVGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGV
EATLKPTKWDGMLASLDSKRIDVVINQVTISDERKKKYDFSTPYTVSGIQALVKKGNEGTIKTANDLKGKKVGVGLGTNY
EEWLRQNVQGVDIRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTKDTLAVTGDAFSRQESGVAVRKNNEDLLKAVN
EAIAEMQKDGTLQALSQKWFGADVTK

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=266, Percent_Identity=93.609022556391, Blast_Score=507, Evalue=1e-145,
Organism=Escherichia coli, GI1787085, Length=220, Percent_Identity=33.1818181818182, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787031, Length=223, Percent_Identity=33.6322869955157, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788649, Length=261, Percent_Identity=29.5019157088123, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787088, Length=252, Percent_Identity=30.1587301587302, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=30.1724137931034, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786876, Length=240, Percent_Identity=26.25, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29125; Mature: 29125

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLALLGRRALLSVMALALVAGMSAKTFADEGLLNKVKERGTLLVGLEGTYPPFSFQGDD
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCC
GKLTGFEVEFAQQLAKHLGVEATLKPTKWDGMLASLDSKRIDVVINQVTISDERKKKYDF
CCEEEEEHHHHHHHHHHCCCCEEECCCCCCCHHHHCCCCCEEEEEEEEEECCHHHHCCCC
STPYTVSGIQALVKKGNEGTIKTANDLKGKKVGVGLGTNYEEWLRQNVQGVDIRTYDDDP
CCCCHHHHHHHHHHCCCCCCEECCCCCCCCEEEEECCCCHHHHHHCCCCCCEEEECCCCC
TKYQDLRVGRIDAILVDRLAALDLVKKTKDTLAVTGDAFSRQESGVAVRKNNEDLLKAVN
CCHHHEECCHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCEEEECCCHHHHHHHH
EAIAEMQKDGTLQALSQKWFGADVTK
HHHHHHHCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKLALLGRRALLSVMALALVAGMSAKTFADEGLLNKVKERGTLLVGLEGTYPPFSFQGDD
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCC
GKLTGFEVEFAQQLAKHLGVEATLKPTKWDGMLASLDSKRIDVVINQVTISDERKKKYDF
CCEEEEEHHHHHHHHHHCCCCEEECCCCCCCHHHHCCCCCEEEEEEEEEECCHHHHCCCC
STPYTVSGIQALVKKGNEGTIKTANDLKGKKVGVGLGTNYEEWLRQNVQGVDIRTYDDDP
CCCCHHHHHHHHHHCCCCCCEECCCCCCCCEEEEECCCCHHHHHHCCCCCCEEEECCCCC
TKYQDLRVGRIDAILVDRLAALDLVKKTKDTLAVTGDAFSRQESGVAVRKNNEDLLKAVN
CCHHHEECCHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCEEEECCCHHHHHHHH
EAIAEMQKDGTLQALSQKWFGADVTK
HHHHHHHCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]