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The map label for this gene is hyfF [H]

Identifier: 218548080

GI number: 218548080

Start: 705760

End: 707340

Strand: Reverse

Name: hyfF [H]

Synonym: EFER_0689

Alternate gene names: 218548080

Gene position: 707340-705760 (Counterclockwise)

Preceding gene: 218548081

Following gene: 218548079

Centisome position: 15.41

GC content: 49.4

Gene sequence:

>1581_bases
ATGAGTTATTCTGCCTTGTTTGCCTTACTCCTGATAACGCCGTTGTTCTTCTCGCTGCTCTGTTTTGCCTGTCGGTTGCT
GGTCAAAGCACAACGCCATGTCGTTACGTTCCTGCATACAACAGGGATTACGTTGTTATTAATCCTCGCACTTTGGTTGG
TGAATATCACTCTCAACGAAGGAGGGATTTTTGCCGCAAATCTGTGGTTACACCTTGATAGCCTGAGTGGCTTATTTCTC
GGTTTGCTTGGGGTGATTGGATTCCTTACCGGTATTTATTCTATTGGCTATATGGGTCATGAGGTGGACCACGGAGAGAT
TTCGCAGAACACGCTGTGTGATTACTACGGTTTTTTCCATCTCTTTTTATTCACCATGTTGTTGGTGGTCACCAGTAATA
ACCTGATTGTGATGTGGGCAGCGATAGAAGCCACAACATTAAGTTCCGCGTTTCTGGTAGGGATCTACGGTCAACGTTCA
TCTCTGGAAGCAGCCTGGAAGTACATCATCATCTGTACTGTAGGTGTCGCTTTCGGTTTATTTGGCACGGTACTGGTCTA
TGCCAATGCAGCCAGCATTATGCCTGATCCAGAACTGGCTATTTTCTGGACAGCGGTGTTGCAACAAGCTCCGTTGCTTG
ATCCGATGTTAATGCTGCTGGCGTTTGTATTTGTGTTGATCGGTTTTGGCACCAAAATCGGTCTGTTCCCGATGCACGCC
TGGCTACCTGACGCTCACAGTGAAGCCCCAAGCCCGGTGAGTGCCCTGCTCTCTGCCGTTTTACTGAATTGCGCGTTACT
GGTACTCATCCGCTACTACGTCATCATTTGCCAGAGCATCGGCAACGACTTCCCGAACAAACTGTTGTTGATATTTGGCA
TGTTGTCCGTTGCTGTAGCCGCGTTTTTCATCCTTGTGCAACGTGATATGAAACGCCTGCTGGCCTATTCCAGTGTGGAA
AATATGGGGCTGGTTGCCGTGGCGTTGGGCATTGGTGGGCCATTAGGAATTCTGGCTGCACTACTCCATACCTTAAATCA
TAGTCTGGCTAAAACGCTGTTGTTCTGTGGTTCTGGCAATGTGTTGCTGAAATACGGCACGCGGGATCTGAATGTTATTC
GCGGCATGTTAAAGATCATGCCATTCAGTGCCGTGTTGCTGGGAGCAGGCGCACTGGCGCTTGCCGGGATGCCACCTTTC
AATATCTTCCTCAGTGAGTTTATGACCATTACCGCCGGGCTGGCGCGTCAACATTTGGTGCTCACCATTGTACTGCTGTT
GTTACTGACTCTGGTGTTGGCAGGGCTGGTGCGGATGGCCGCACGGGTGTTGTTTGCCAGGCCACCAGAAGCGGTGATTC
GTGGAGAAATAAGCTGGCTCACCACCACGCCAATGGCTTTGCTGGTGGTCATGATGCTGATGATGGGGACAGATATTCCT
CAGCCGATCATCAGGATTCTCCAAAACGCATCAGTTATTGTCCTCGCAGGGACGGATGAATCTCCGGCACAAATTATGAG
CTGGCAAGACTTCATCACAACAGACACGGCTTCATTACCGGAGAGTAAAAGTGAACGTTAA

Upstream 100 bases:

>100_bases
ATCTTTGCCGTGATTGTGATGGTGCTGCTGGCAAGAAAGATTTGGCGTACGCATGGCACGCTGGATGTGAACACTCTTAC
TGCGCTGAAAGGATAATGAC

Downstream 100 bases:

>100_bases
TACCACAAATAATCGCGGCGAAGCGTGTCTGGCCGCCCTGAAAATACAATTTCCCGGCGCGGTGCTGGACGAAGAACGAC
AAACCTCCGAGCAGGTCACT

Product: hydrogenase 4 subunit F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSYSALFALLLITPLFFSLLCFACRLLVKAQRHVVTFLHTTGITLLLILALWLVNITLNEGGIFAANLWLHLDSLSGLFL
GLLGVIGFLTGIYSIGYMGHEVDHGEISQNTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
SLEAAWKYIIICTVGVAFGLFGTVLVYANAASIMPDPELAIFWTAVLQQAPLLDPMLMLLAFVFVLIGFGTKIGLFPMHA
WLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYVIICQSIGNDFPNKLLLIFGMLSVAVAAFFILVQRDMKRLLAYSSVE
NMGLVAVALGIGGPLGILAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVIRGMLKIMPFSAVLLGAGALALAGMPPF
NIFLSEFMTITAGLARQHLVLTIVLLLLLTLVLAGLVRMAARVLFARPPEAVIRGEISWLTTTPMALLVVMMLMMGTDIP
QPIIRILQNASVIVLAGTDESPAQIMSWQDFITTDTASLPESKSER

Sequences:

>Translated_526_residues
MSYSALFALLLITPLFFSLLCFACRLLVKAQRHVVTFLHTTGITLLLILALWLVNITLNEGGIFAANLWLHLDSLSGLFL
GLLGVIGFLTGIYSIGYMGHEVDHGEISQNTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
SLEAAWKYIIICTVGVAFGLFGTVLVYANAASIMPDPELAIFWTAVLQQAPLLDPMLMLLAFVFVLIGFGTKIGLFPMHA
WLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYVIICQSIGNDFPNKLLLIFGMLSVAVAAFFILVQRDMKRLLAYSSVE
NMGLVAVALGIGGPLGILAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVIRGMLKIMPFSAVLLGAGALALAGMPPF
NIFLSEFMTITAGLARQHLVLTIVLLLLLTLVLAGLVRMAARVLFARPPEAVIRGEISWLTTTPMALLVVMMLMMGTDIP
QPIIRILQNASVIVLAGTDESPAQIMSWQDFITTDTASLPESKSER
>Mature_525_residues
SYSALFALLLITPLFFSLLCFACRLLVKAQRHVVTFLHTTGITLLLILALWLVNITLNEGGIFAANLWLHLDSLSGLFLG
LLGVIGFLTGIYSIGYMGHEVDHGEISQNTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSS
LEAAWKYIIICTVGVAFGLFGTVLVYANAASIMPDPELAIFWTAVLQQAPLLDPMLMLLAFVFVLIGFGTKIGLFPMHAW
LPDAHSEAPSPVSALLSAVLLNCALLVLIRYYVIICQSIGNDFPNKLLLIFGMLSVAVAAFFILVQRDMKRLLAYSSVEN
MGLVAVALGIGGPLGILAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVIRGMLKIMPFSAVLLGAGALALAGMPPFN
IFLSEFMTITAGLARQHLVLTIVLLLLLTLVLAGLVRMAARVLFARPPEAVIRGEISWLTTTPMALLVVMMLMMGTDIPQ
PIIRILQNASVIVLAGTDESPAQIMSWQDFITTDTASLPESKSER

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=383, Percent_Identity=28.9817232375979, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI251831116, Length=184, Percent_Identity=31.5217391304348, Blast_Score=76, Evalue=8e-14,
Organism=Escherichia coli, GI1788831, Length=526, Percent_Identity=81.1787072243346, Blast_Score=813, Evalue=0.0,
Organism=Escherichia coli, GI1788827, Length=357, Percent_Identity=31.6526610644258, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788829, Length=393, Percent_Identity=30.2798982188295, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788613, Length=524, Percent_Identity=25.9541984732824, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788614, Length=359, Percent_Identity=28.9693593314763, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI2367154, Length=354, Percent_Identity=28.5310734463277, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI145693160, Length=205, Percent_Identity=31.219512195122, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 57069; Mature: 56938

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYSALFALLLITPLFFSLLCFACRLLVKAQRHVVTFLHTTGITLLLILALWLVNITLNE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
GGIFAANLWLHLDSLSGLFLGLLGVIGFLTGIYSIGYMGHEVDHGEISQNTLCDYYGFFH
CCEEEEEHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
LFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSSLEAAWKYIIICTVGVAFGL
HHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHH
FGTVLVYANAASIMPDPELAIFWTAVLQQAPLLDPMLMLLAFVFVLIGFGTKIGLFPMHA
HHHHHHHHCHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHH
WLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYVIICQSIGNDFPNKLLLIFGMLSVAVA
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AFFILVQRDMKRLLAYSSVENMGLVAVALGIGGPLGILAALLHTLNHSLAKTLLFCGSGN
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
VLLKYGTRDLNVIRGMLKIMPFSAVLLGAGALALAGMPPFNIFLSEFMTITAGLARQHLV
EEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LTIVLLLLLTLVLAGLVRMAARVLFARPPEAVIRGEISWLTTTPMALLVVMMLMMGTDIP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCC
QPIIRILQNASVIVLAGTDESPAQIMSWQDFITTDTASLPESKSER
HHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SYSALFALLLITPLFFSLLCFACRLLVKAQRHVVTFLHTTGITLLLILALWLVNITLNE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
GGIFAANLWLHLDSLSGLFLGLLGVIGFLTGIYSIGYMGHEVDHGEISQNTLCDYYGFFH
CCEEEEEHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
LFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSSLEAAWKYIIICTVGVAFGL
HHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHH
FGTVLVYANAASIMPDPELAIFWTAVLQQAPLLDPMLMLLAFVFVLIGFGTKIGLFPMHA
HHHHHHHHCHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHH
WLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYVIICQSIGNDFPNKLLLIFGMLSVAVA
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AFFILVQRDMKRLLAYSSVENMGLVAVALGIGGPLGILAALLHTLNHSLAKTLLFCGSGN
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
VLLKYGTRDLNVIRGMLKIMPFSAVLLGAGALALAGMPPFNIFLSEFMTITAGLARQHLV
EEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LTIVLLLLLTLVLAGLVRMAARVLFARPPEAVIRGEISWLTTTPMALLVVMMLMMGTDIP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCC
QPIIRILQNASVIVLAGTDESPAQIMSWQDFITTDTASLPESKSER
HHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]