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The map label for this gene is hyfE [H]

Identifier: 218548081

GI number: 218548081

Start: 707345

End: 707995

Strand: Reverse

Name: hyfE [H]

Synonym: EFER_0690

Alternate gene names: 218548081

Gene position: 707995-707345 (Counterclockwise)

Preceding gene: 218548082

Following gene: 218548080

Centisome position: 15.43

GC content: 50.23

Gene sequence:

>651_bases
ATGACGGGTATATCAATTGTTAATAACCTGGCAGGACTCATGATGCTGACTTCACTGTTCGTGATCAGCGTGAAAGGCTT
TCGCCTTGCCTGTGTCTTTTACGCTTTTCAGTCACTGGTGTTGGTTTCAATTTTCCTGACTCTTGCCCAATTATTTCAGG
CAGAGGAACTGCTGATGTGGTCTGGCAGTGCATTTATCACCAAAGTACTCCTCGTGCCGTTAATCATGATCTGGGCTGTA
CGCAAAATAACGGTCAACAGGAGCGGGAAGGCGTTGTTCAGCCCGGTCATTATGGCGCTACTGGCTGCGCTGATAGTTCT
GCTGTGTGCCTTTGTCATCCGCCCGGTGACATTACCCATGGCTATTGGCCTTAAGCCTGCACTGGCTGTAGCACTGGGTC
ACTTCTTACTGGGCCTGCTGTGCATCATTAGTCAGCGCAATATTCTGCGTCAGGTATTTGGTTACTGCCTGATGGAAAAC
GGCTCACATCTGGTCCTTGCTCTCCTTGCCTGGCGTGCACCGGAACTGGTGGAAATTGGTATCGCTACTGACGCCATCTT
TGCCGTGATTGTGATGGTGCTGCTGGCAAGAAAGATTTGGCGTACGCATGGCACGCTGGATGTGAACACTCTTACTGCGC
TGAAAGGATAA

Upstream 100 bases:

>100_bases
CGTTGCCGTTTTCTATGCGTCTGGTGCTGATTGTGCTCATTGTGATGTCGCTTATCTCCAGCGTAATCGCTGCTGTATGG
CTTCAGTAAGGAGTTGAAAT

Downstream 100 bases:

>100_bases
TGACATGAGTTATTCTGCCTTGTTTGCCTTACTCCTGATAACGCCGTTGTTCTTCTCGCTGCTCTGTTTTGCCTGTCGGT
TGCTGGTCAAAGCACAACGC

Product: hydrogenase 4 membrane subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MTGISIVNNLAGLMMLTSLFVISVKGFRLACVFYAFQSLVLVSIFLTLAQLFQAEELLMWSGSAFITKVLLVPLIMIWAV
RKITVNRSGKALFSPVIMALLAALIVLLCAFVIRPVTLPMAIGLKPALAVALGHFLLGLLCIISQRNILRQVFGYCLMEN
GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNTLTALKG

Sequences:

>Translated_216_residues
MTGISIVNNLAGLMMLTSLFVISVKGFRLACVFYAFQSLVLVSIFLTLAQLFQAEELLMWSGSAFITKVLLVPLIMIWAV
RKITVNRSGKALFSPVIMALLAALIVLLCAFVIRPVTLPMAIGLKPALAVALGHFLLGLLCIISQRNILRQVFGYCLMEN
GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNTLTALKG
>Mature_215_residues
TGISIVNNLAGLMMLTSLFVISVKGFRLACVFYAFQSLVLVSIFLTLAQLFQAEELLMWSGSAFITKVLLVPLIMIWAVR
KITVNRSGKALFSPVIMALLAALIVLLCAFVIRPVTLPMAIGLKPALAVALGHFLLGLLCIISQRNILRQVFGYCLMENG
SHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNTLTALKG

Specific function: Unknown

COG id: COG4237

COG function: function code C; Hydrogenase 4 membrane component (E)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788830, Length=216, Percent_Identity=84.7222222222222, Blast_Score=334, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001133 [H]

Pfam domain/function: PF00420 Oxidored_q2 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 23548; Mature: 23417

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGISIVNNLAGLMMLTSLFVISVKGFRLACVFYAFQSLVLVSIFLTLAQLFQAEELLMW
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGSAFITKVLLVPLIMIWAVRKITVNRSGKALFSPVIMALLAALIVLLCAFVIRPVTLPM
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIGLKPALAVALGHFLLGLLCIISQRNILRQVFGYCLMENGSHLVLALLAWRAPELVEIG
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHH
IATDAIFAVIVMVLLARKIWRTHGTLDVNTLTALKG
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC
>Mature Secondary Structure 
TGISIVNNLAGLMMLTSLFVISVKGFRLACVFYAFQSLVLVSIFLTLAQLFQAEELLMW
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGSAFITKVLLVPLIMIWAVRKITVNRSGKALFSPVIMALLAALIVLLCAFVIRPVTLPM
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIGLKPALAVALGHFLLGLLCIISQRNILRQVFGYCLMENGSHLVLALLAWRAPELVEIG
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHH
IATDAIFAVIVMVLLARKIWRTHGTLDVNTLTALKG
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]