Click here to switch to the map view.

The map label for this gene is hyfG [H]

Identifier: 218548079

GI number: 218548079

Start: 704055

End: 705770

Strand: Reverse

Name: hyfG [H]

Synonym: EFER_0688

Alternate gene names: 218548079

Gene position: 705770-704055 (Counterclockwise)

Preceding gene: 218548080

Following gene: 218548078

Centisome position: 15.38

GC content: 51.98

Gene sequence:

>1716_bases
GTGAACGTTAATACCACAAATAATCGCGGCGAAGCGTGTCTGGCCGCCCTGAAAATACAATTTCCCGGCGCGGTGCTGGA
CGAAGAACGACAAACCTCCGAGCAGGTCACTATTACGGTAAAAATTAACCTGCTGCCTGATGTTGTTCATTATCTTTATT
ATCAACACGATGGTTGGTTACCGGTACTTTTTGGCAATGATGAACGAACTTTAAATGGTCACTACGCCGTTTACTATGCC
CTTTCGATGGAAGGGACAGAAAAGTGTTGGATTGTGGTGAAAGCGCTGGTTGATGCCGAAAGTCGGGAATTTCCCTCTGT
TACACCACGCGTACCCGCTGCCGTTTGGGGGGAGCGTGAAATTCGTGATATGTACGGCCTCATTCCTGTGGGTCTGCCAG
ATCAGCGACGCCTGGTGTTGCCGGATGACTGGCCGGAAGATATGCATCCGTTGCGAAAAGACGCGATGGATTATCGCCTG
CGCCCGGAGCCGACAACTGATGTCAAAACATATCCGTTTATTAATGAAGGTGATAGTGACGCGCGAGTGATCCCTATTGG
CCCGCTGCATATCACTTCCGATGAACCCGGTCACTTCCGCCTGTTCGTTGATGGCGAACAGATTGTTGATGCCGATTACC
GCCTGTTTTATGTCCATCGTGGCATGGAAAAACTGGCGGAAACGCGCATGGGCTATAATGAAGTGACCTTCTTATCTGAC
CGCGTATGCGGTATCTGTGGCTTTGCGCACAGCGTAGCCTATACCACCTCGGTGGAAAATGCGCTGGGCATTGTGGTGCC
GCAACGGGCACACACTATCCGTTCGATTCTTCTGGAAGTAGAACGCCTGCATAGCCATCTGCTTAACCTTGGCCTCTCCT
GTCATTTCGTCGGTTTTGATACCGGCTTTATGCAATTTTTCCGTGTGCGGGAAAAGTCGATGACAATGGCTGAGTTGCTG
ACCGGGTCACGTAAAACCTATGGGCTGAATCTGATTGGTGGCGTGCGACGCGATATTCTTAAAGAGCAGCGACTGCAAAC
GCTGAAACTGGTCCGTGAGATGCGTGCCGATGTGTCAGAGTTGGTGGAGATGCTGCTTGCCACGCCGAATATGGAACAGC
GTACCCAGGGAATTGGTATTCTCGACCGACAAATCGCCCGCGATTACAGCCCCGTTGGGCCGTTGATTCGTGGCAGTGGT
TTTGCCCGTGACTTGCGCTTTGATCACCCCTACGCCGACTATGGCAACATTCCCAAAACACTGTTTACCTTCTCTGGCGG
CGATGTGTTCTCCCGCGTGATGGTGCGCGTCAAAGAAACGTTCGATTCGCTGGCGATGCTGGAATATGCCCTCGACAACA
TGCCGAATACGCCGTTGCTTACTGAAGGTTTTAGCTATAAACCACACGCATTCGCGCTGGGCTTTGTGGAAGCACCACGC
GGCGAAGATGTGCACTGGAGCATGCTGGGTGATAACCAGAAATTATTCCGCTGGCGCTGTCGTGCTGCTACCTACGCTAA
CTGGCCAGTCCTGCGCTATATGCTACGCGGTAATACCGTTTCTGATGCCCCCCTGATTATCGGCAGCCTTGATCCCTGCT
ATTCCTGTACTGACCGCGTGACGCTGGTTGATGTGCGTAAGCATCAGTCAAAAACCGTGCCGTATAAAGAGATCGAGCGC
TATGGCATTGATCGTAACCGCTCACCGCTGAAGTAA

Upstream 100 bases:

>100_bases
TCAGTTATTGTCCTCGCAGGGACGGATGAATCTCCGGCACAAATTATGAGCTGGCAAGACTTCATCACAACAGACACGGC
TTCATTACCGGAGAGTAAAA

Downstream 100 bases:

>100_bases
GGACAGAAAATGCTGAAATTACTCAAAACCATTATGCGCGCCGGTGAAGCAACGGTGAAATATCCTTTCGCGCCGCTGGA
AGTTAGCCCTGGTTTTCGGG

Product: hydrogenase 4 subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MNVNTTNNRGEACLAALKIQFPGAVLDEERQTSEQVTITVKINLLPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYA
LSMEGTEKCWIVVKALVDAESREFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
RPEPTTDVKTYPFINEGDSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD
RVCGICGFAHSVAYTTSVENALGIVVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELL
TGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPVGPLIRGSG
FARDLRFDHPYADYGNIPKTLFTFSGGDVFSRVMVRVKETFDSLAMLEYALDNMPNTPLLTEGFSYKPHAFALGFVEAPR
GEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKHQSKTVPYKEIER
YGIDRNRSPLK

Sequences:

>Translated_571_residues
MNVNTTNNRGEACLAALKIQFPGAVLDEERQTSEQVTITVKINLLPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYA
LSMEGTEKCWIVVKALVDAESREFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
RPEPTTDVKTYPFINEGDSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD
RVCGICGFAHSVAYTTSVENALGIVVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELL
TGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPVGPLIRGSG
FARDLRFDHPYADYGNIPKTLFTFSGGDVFSRVMVRVKETFDSLAMLEYALDNMPNTPLLTEGFSYKPHAFALGFVEAPR
GEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKHQSKTVPYKEIER
YGIDRNRSPLK
>Mature_571_residues
MNVNTTNNRGEACLAALKIQFPGAVLDEERQTSEQVTITVKINLLPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYA
LSMEGTEKCWIVVKALVDAESREFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
RPEPTTDVKTYPFINEGDSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD
RVCGICGFAHSVAYTTSVENALGIVVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELL
TGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPVGPLIRGSG
FARDLRFDHPYADYGNIPKTLFTFSGGDVFSRVMVRVKETFDSLAMLEYALDNMPNTPLLTEGFSYKPHAFALGFVEAPR
GEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKHQSKTVPYKEIER
YGIDRNRSPLK

Specific function: Unknown

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=382, Percent_Identity=26.4397905759162, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI260898743, Length=374, Percent_Identity=27.0053475935829, Blast_Score=112, Evalue=7e-25,
Organism=Escherichia coli, GI1788832, Length=571, Percent_Identity=93.6952714535902, Blast_Score=1105, Evalue=0.0,
Organism=Escherichia coli, GI1789076, Length=563, Percent_Identity=73.3570159857904, Blast_Score=889, Evalue=0.0,
Organism=Escherichia coli, GI145693162, Length=530, Percent_Identity=27.7358490566038, Blast_Score=162, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17555284, Length=392, Percent_Identity=25.5102040816327, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17568379, Length=392, Percent_Identity=25, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI221459469, Length=385, Percent_Identity=26.7532467532467, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24638644, Length=381, Percent_Identity=27.2965879265092, Blast_Score=136, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 65111; Mature: 65111

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00535 COMPLEX1_49K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVNTTNNRGEACLAALKIQFPGAVLDEERQTSEQVTITVKINLLPDVVHYLYYQHDGWL
CCCCCCCCCCCEEEEEEEEECCCCCCCCHHCCCCEEEEEEEEECHHHHHHHHHEECCCCE
PVLFGNDERTLNGHYAVYYALSMEGTEKCWIVVKALVDAESREFPSVTPRVPAAVWGERE
EEEECCCCCEECCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCHH
IRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRLRPEPTTDVKTYPFINEGDSD
HHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCEECCCCCCCCEECCCCCCCCCC
ARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD
CEEEEECCEEECCCCCCEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCEEEHHH
RVCGICGFAHSVAYTTSVENALGIVVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFD
HHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
TGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSE
HHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVEMLLATPNMEQRTQGIGILDRQIARDYSPVGPLIRGSGFARDLRFDHPYADYGNIPKT
HHHHHHCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
LFTFSGGDVFSRVMVRVKETFDSLAMLEYALDNMPNTPLLTEGFSYKPHAFALGFVEAPR
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEEEEECCC
GEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRV
CCCEEEEEECCCHHHHHEEHHHCCCCCCHHHHHHHCCCCCCCCCEEEECCCCHHCCCCCE
TLVDVRKHQSKTVPYKEIERYGIDRNRSPLK
EEEEEHHCCCCCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNVNTTNNRGEACLAALKIQFPGAVLDEERQTSEQVTITVKINLLPDVVHYLYYQHDGWL
CCCCCCCCCCCEEEEEEEEECCCCCCCCHHCCCCEEEEEEEEECHHHHHHHHHEECCCCE
PVLFGNDERTLNGHYAVYYALSMEGTEKCWIVVKALVDAESREFPSVTPRVPAAVWGERE
EEEECCCCCEECCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCHH
IRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRLRPEPTTDVKTYPFINEGDSD
HHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCEECCCCCCCCEECCCCCCCCCC
ARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD
CEEEEECCEEECCCCCCEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCEEEHHH
RVCGICGFAHSVAYTTSVENALGIVVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFD
HHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
TGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSE
HHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVEMLLATPNMEQRTQGIGILDRQIARDYSPVGPLIRGSGFARDLRFDHPYADYGNIPKT
HHHHHHCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
LFTFSGGDVFSRVMVRVKETFDSLAMLEYALDNMPNTPLLTEGFSYKPHAFALGFVEAPR
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEEEEECCC
GEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRV
CCCEEEEEECCCHHHHHEEHHHCCCCCCHHHHHHHCCCCCCCCCEEEECCCCHHCCCCCE
TLVDVRKHQSKTVPYKEIERYGIDRNRSPLK
EEEEEHHCCCCCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]