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The map label for this gene is topB [H]

Identifier: 218231865

GI number: 218231865

Start: 422390

End: 422722

Strand: Reverse

Name: topB [H]

Synonym: BCB4264_A0437

Alternate gene names: 218231865

Gene position: 422722-422390 (Counterclockwise)

Preceding gene: 218233495

Following gene: 218231878

Centisome position: 7.8

GC content: 33.93

Gene sequence:

>333_bases
ATGTCAAAAAGCGTTGTAATCGCTGAAAAACCTTCCGTTGCACGAGATATTGCTCGTGTACTGAAGTGTGATAAAAAAGG
AAACGGCTATCTTGAAGGTAGTAAATATATTGTAACTTGGGCTTTAGGTCATCTCGTTACATTAGCAGATCCAGAAAGTT
ATGATGTGAAATATAAAAAGTGGAATTTAGAAGATTTACCGATGCTACCTGAGCGTTTGAAATTAACGGTTATTAAACAA
ACTGGTAAACAATTTAATGCTGTAAAAAGTCAGCTACTTAGAAAAGATGTAAATGAAATTATTGTAGCAACAGACGGAGA
CTTATCTCTATAA

Upstream 100 bases:

>100_bases
TGTTATCCCATTAAAAACCGTTCAAATATGGAGCGAAACTGAATCTCAAGAAGATGTATGCTATATTTAACTTATGGAAA
TTTGAAAGGATGTTTTTAAT

Downstream 100 bases:

>100_bases
AATATACCTGTACAAAAATCCTGAAACATACTTAATCCCTTGTCTAATGAAGGTATTTTCTTTTTTATAATTGTACATAA
ATCACAAAAATATTAATTTA

Product: DNA topoisomerase 3

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 110; Mature: 109

Protein sequence:

>110_residues
MSKSVVIAEKPSVARDIARVLKCDKKGNGYLEGSKYIVTWALGHLVTLADPESYDVKYKKWNLEDLPMLPERLKLTVIKQ
TGKQFNAVKSQLLRKDVNEIIVATDGDLSL

Sequences:

>Translated_110_residues
MSKSVVIAEKPSVARDIARVLKCDKKGNGYLEGSKYIVTWALGHLVTLADPESYDVKYKKWNLEDLPMLPERLKLTVIKQ
TGKQFNAVKSQLLRKDVNEIIVATDGDLSL
>Mature_109_residues
SKSVVIAEKPSVARDIARVLKCDKKGNGYLEGSKYIVTWALGHLVTLADPESYDVKYKKWNLEDLPMLPERLKLTVIKQT
GKQFNAVKSQLLRKDVNEIIVATDGDLSL

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1788061, Length=103, Percent_Identity=46.6019417475728, Blast_Score=92, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 12346; Mature: 12214

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSVVIAEKPSVARDIARVLKCDKKGNGYLEGSKYIVTWALGHLVTLADPESYDVKYKK
CCCCEEEEECCCHHHHHHHHHHCCCCCCCEECCCCEEEEEECCCEEEECCCCCCCEEEEE
WNLEDLPMLPERLKLTVIKQTGKQFNAVKSQLLRKDVNEIIVATDGDLSL
ECCCCCCCCCCHHEEEHHHHCCHHHHHHHHHHHHHCHHHEEEECCCCCCC
>Mature Secondary Structure 
SKSVVIAEKPSVARDIARVLKCDKKGNGYLEGSKYIVTWALGHLVTLADPESYDVKYKK
CCCEEEEECCCHHHHHHHHHHCCCCCCCEECCCCEEEEEECCCEEEECCCCCCCEEEEE
WNLEDLPMLPERLKLTVIKQTGKQFNAVKSQLLRKDVNEIIVATDGDLSL
ECCCCCCCCCCHHEEEHHHHCCHHHHHHHHHHHHHCHHHEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA