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The map label for this gene is fumB [H]

Identifier: 209551981

GI number: 209551981

Start: 50544

End: 52055

Strand: Reverse

Name: fumB [H]

Synonym: Rleg2_6119

Alternate gene names: 209551981

Gene position: 52055-50544 (Counterclockwise)

Preceding gene: 209551983

Following gene: 209551980

Centisome position: 16.86

GC content: 60.12

Gene sequence:

>1512_bases
ATGCGGAAGATCAAAGCTGAGCACATCTCAACGAGCGTGTCGGACGCGCTCCAGTATATAAGCTATTACCACCCTCTGGA
CTACATCCGGGCTCTCGGCGACGCGTATCATATCGAGGAATCGCCAGCAGCCAAAGACGCGATAGCCCAGATCCTGATGA
ACAGCCGGATGTCCGCAGAGGGGCACCGACCGATATGTCAGGATACCGGAATCGTCACCGTTTTCGTAAAGTGGGGCCAG
GACTGTCTCCTGGACTCGAGCCAGAGCCTTCAGGCAATCATCGATGAGGGCGTCCGAAATGCATACCTCGATCCAACGAA
CCCACTGCGTGCCTCAGTGGTAGCCGATCCGCTCTTTGCCCGACGCAACACGCGCGACAACACTCCGAGTGTCCTACATG
TGGAAATGATGCCAGGCGCCACGGTCGAAATAACCGTCGCTGCAAAAGGTGGTGGATCGGAAAACAAAGCCAAGTTCAAA
ATGCTCAATCCGTCCGATTCCCTAATCGACTGGGTTCTCGAGACAGTCCCAGCGATGGGAGCCGGCTGGTGTCCGCCTGG
CATGCTCGGCATCGGCGTGGGGGGTACGGCGGAGAAGGCCATGCTTCTGGCGAAAGAGTCACTGATGGCTCCTATAAACA
TGGCTCAGTTGAAAGCTCGCGGCCCGATCAGTCCGCTTGAGGAACTAAGGATCGAGCTTCACGACCGGATTAATTCGCTC
GGCATCGGCGCGCAGGGGCTCGGCGGACTGACGACCGTCCTGGATGTTAAGATCCTGGACTGGCCCACCCATGCCGCGTC
GAAGCCCGTCGCTCTCATACCGAACTGCGCCGCGACCCGGCACGCGCATTTCACCCTTGACGGGTCTGGCCCGGCGTTTC
TTCCCCAACCCGATCTTTCAGCATGGCCGCAGGTCAGATGGAAACCGGACGAAGGCTCCAGGCGAGTTCAGCTGGACACC
CTGACACCCGAAGAGGTCGGGTCTTGGAAGGCCGGAGACAGGCTATTGCTTTCAGGTCGGATGCTTACCGGGCGCGACGC
GGCCCACAAGCGGATTCAAGATCTGCTCGCCGACAACAGACCGTTGCCCGTCGATTTCAAAAACCGCGTCATCTATTACG
TCGGCCCGGTCGACCCCGTTGGCAGCGAGGTGGTCGGTCCGGCGGGGCCCACCACGTCCACCAGAATGGACAAGTTCACC
GACATGATGCTTGGTCTCGGCCTGCTAGCTATGGTCGGGAAGGCGGAACGGGGGGCGTCCGCGGTCGCTTCGCTGAAGCG
CCACAAACGCGCCTATCTCACAGCGGTGGGGGGGGCGGCATATCTCGTATCGCGGGCGATACGGTCGAGCCGCATAGTCG
CCTTCGAGGATCTCGGGATGGAGGCCATATACGAATTCGACGTCCATGAAATGCCCGTAACCGTTGCGGTTACGACAGAA
GGTGAGAACGTCCACGTCTCGGGCCCCGGCAAGTGGCAGCGTCCGCTCATTAACGGCTCGCGTGGCAGCTGA

Upstream 100 bases:

>100_bases
CACGCTATCTTTCCCCGGGGCGTATTCCGGAAGAAAACCAAGCGCAGGCGAAAGCCGCCGGTGCTTCCGCTCGCCAATAT
AAGGCAACGGGAGATGATAA

Downstream 100 bases:

>100_bases
GAGCTGTTGCTTGGCGCGCGACGGAAGGTCAAAGACATATATGGTGCGCCCCGTGGCCTAAGAATCCATAAGCAGCGTCT
ATCAGGGAGACTTGCTCTGG

Product: hydro-lyase, Fe-S type, tartrate/fumarate subfamily, subunit alpha

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MRKIKAEHISTSVSDALQYISYYHPLDYIRALGDAYHIEESPAAKDAIAQILMNSRMSAEGHRPICQDTGIVTVFVKWGQ
DCLLDSSQSLQAIIDEGVRNAYLDPTNPLRASVVADPLFARRNTRDNTPSVLHVEMMPGATVEITVAAKGGGSENKAKFK
MLNPSDSLIDWVLETVPAMGAGWCPPGMLGIGVGGTAEKAMLLAKESLMAPINMAQLKARGPISPLEELRIELHDRINSL
GIGAQGLGGLTTVLDVKILDWPTHAASKPVALIPNCAATRHAHFTLDGSGPAFLPQPDLSAWPQVRWKPDEGSRRVQLDT
LTPEEVGSWKAGDRLLLSGRMLTGRDAAHKRIQDLLADNRPLPVDFKNRVIYYVGPVDPVGSEVVGPAGPTTSTRMDKFT
DMMLGLGLLAMVGKAERGASAVASLKRHKRAYLTAVGGAAYLVSRAIRSSRIVAFEDLGMEAIYEFDVHEMPVTVAVTTE
GENVHVSGPGKWQRPLINGSRGS

Sequences:

>Translated_503_residues
MRKIKAEHISTSVSDALQYISYYHPLDYIRALGDAYHIEESPAAKDAIAQILMNSRMSAEGHRPICQDTGIVTVFVKWGQ
DCLLDSSQSLQAIIDEGVRNAYLDPTNPLRASVVADPLFARRNTRDNTPSVLHVEMMPGATVEITVAAKGGGSENKAKFK
MLNPSDSLIDWVLETVPAMGAGWCPPGMLGIGVGGTAEKAMLLAKESLMAPINMAQLKARGPISPLEELRIELHDRINSL
GIGAQGLGGLTTVLDVKILDWPTHAASKPVALIPNCAATRHAHFTLDGSGPAFLPQPDLSAWPQVRWKPDEGSRRVQLDT
LTPEEVGSWKAGDRLLLSGRMLTGRDAAHKRIQDLLADNRPLPVDFKNRVIYYVGPVDPVGSEVVGPAGPTTSTRMDKFT
DMMLGLGLLAMVGKAERGASAVASLKRHKRAYLTAVGGAAYLVSRAIRSSRIVAFEDLGMEAIYEFDVHEMPVTVAVTTE
GENVHVSGPGKWQRPLINGSRGS
>Mature_503_residues
MRKIKAEHISTSVSDALQYISYYHPLDYIRALGDAYHIEESPAAKDAIAQILMNSRMSAEGHRPICQDTGIVTVFVKWGQ
DCLLDSSQSLQAIIDEGVRNAYLDPTNPLRASVVADPLFARRNTRDNTPSVLHVEMMPGATVEITVAAKGGGSENKAKFK
MLNPSDSLIDWVLETVPAMGAGWCPPGMLGIGVGGTAEKAMLLAKESLMAPINMAQLKARGPISPLEELRIELHDRINSL
GIGAQGLGGLTTVLDVKILDWPTHAASKPVALIPNCAATRHAHFTLDGSGPAFLPQPDLSAWPQVRWKPDEGSRRVQLDT
LTPEEVGSWKAGDRLLLSGRMLTGRDAAHKRIQDLLADNRPLPVDFKNRVIYYVGPVDPVGSEVVGPAGPTTSTRMDKFT
DMMLGLGLLAMVGKAERGASAVASLKRHKRAYLTAVGGAAYLVSRAIRSSRIVAFEDLGMEAIYEFDVHEMPVTVAVTTE
GENVHVSGPGKWQRPLINGSRGS

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=464, Percent_Identity=28.6637931034483, Blast_Score=175, Evalue=7e-45,
Organism=Escherichia coli, GI1787897, Length=471, Percent_Identity=29.9363057324841, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI1789442, Length=286, Percent_Identity=29.3706293706294, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789443, Length=177, Percent_Identity=27.683615819209, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54286; Mature: 54286

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIKAEHISTSVSDALQYISYYHPLDYIRALGDAYHIEESPAAKDAIAQILMNSRMSAE
CCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHCCCCCC
GHRPICQDTGIVTVFVKWGQDCLLDSSQSLQAIIDEGVRNAYLDPTNPLRASVVADPLFA
CCCCCCCCCCEEEEEEECCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCHHHH
RRNTRDNTPSVLHVEMMPGATVEITVAAKGGGSENKAKFKMLNPSDSLIDWVLETVPAMG
HCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCCC
AGWCPPGMLGIGVGGTAEKAMLLAKESLMAPINMAQLKARGPISPLEELRIELHDRINSL
CCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHC
GIGAQGLGGLTTVLDVKILDWPTHAASKPVALIPNCAATRHAHFTLDGSGPAFLPQPDLS
CCCCCCCCCEEEEEEEEEEECCCCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCCCCC
AWPQVRWKPDEGSRRVQLDTLTPEEVGSWKAGDRLLLSGRMLTGRDAAHKRIQDLLADNR
CCCCEEEECCCCCCEEEEECCCHHHHCCCCCCCEEEEECCEECCCHHHHHHHHHHHHCCC
PLPVDFKNRVIYYVGPVDPVGSEVVGPAGPTTSTRMDKFTDMMLGLGLLAMVGKAERGAS
CCCEECCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
AVASLKRHKRAYLTAVGGAAYLVSRAIRSSRIVAFEDLGMEAIYEFDVHEMPVTVAVTTE
HHHHHHHHHHHEEEECCHHHHHHHHHHHHCCEEEEHHCCCCEEEECCCCCCCEEEEEEEC
GENVHVSGPGKWQRPLINGSRGS
CCEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKIKAEHISTSVSDALQYISYYHPLDYIRALGDAYHIEESPAAKDAIAQILMNSRMSAE
CCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHCCCCCC
GHRPICQDTGIVTVFVKWGQDCLLDSSQSLQAIIDEGVRNAYLDPTNPLRASVVADPLFA
CCCCCCCCCCEEEEEEECCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCHHHH
RRNTRDNTPSVLHVEMMPGATVEITVAAKGGGSENKAKFKMLNPSDSLIDWVLETVPAMG
HCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCCC
AGWCPPGMLGIGVGGTAEKAMLLAKESLMAPINMAQLKARGPISPLEELRIELHDRINSL
CCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHC
GIGAQGLGGLTTVLDVKILDWPTHAASKPVALIPNCAATRHAHFTLDGSGPAFLPQPDLS
CCCCCCCCCEEEEEEEEEEECCCCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCCCCC
AWPQVRWKPDEGSRRVQLDTLTPEEVGSWKAGDRLLLSGRMLTGRDAAHKRIQDLLADNR
CCCCEEEECCCCCCEEEEECCCHHHHCCCCCCCEEEEECCEECCCHHHHHHHHHHHHCCC
PLPVDFKNRVIYYVGPVDPVGSEVVGPAGPTTSTRMDKFTDMMLGLGLLAMVGKAERGAS
CCCEECCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
AVASLKRHKRAYLTAVGGAAYLVSRAIRSSRIVAFEDLGMEAIYEFDVHEMPVTVAVTTE
HHHHHHHHHHHEEEECCHHHHHHHHHHHHCCEEEEHHCCCCEEEECCCCCCCEEEEEEEC
GENVHVSGPGKWQRPLINGSRGS
CCEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]