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The map label for this gene is citF [H]

Identifier: 209551983

GI number: 209551983

Start: 53128

End: 54654

Strand: Reverse

Name: citF [H]

Synonym: Rleg2_6121

Alternate gene names: 209551983

Gene position: 54654-53128 (Counterclockwise)

Preceding gene: 209551984

Following gene: 209551981

Centisome position: 17.7

GC content: 58.61

Gene sequence:

>1527_bases
ATGGTGCCCATTCCGTCTGTAATTCCTGGTTATGGGCCAGTGAGAGCGTTTGTATCCGCCGCCCTGGCAACGCCAGAAGC
GAAGGGGGCCAATCTGCCGGAAAGACGAAACGGCGGTCATGACAAACGTGCCGCGAGCCTGCGCGACGCGATCATCGCGT
CGAACCTGCGGAACGGGGGTACGGTCTCCTTCCATCATCACCTTCGTGACGGGGACGACGTGCTTCGCAAGGTCATGACG
GAAATAGCGGAACTCGGGCTTAGAGACTTACGCGTGGCCGCCACGTCGATCTTTCCCGTACACGCGCCCCTGGTCGACTA
TATTCGTTCGGGAGTGGTCAGCAGGATCGTCTCGAGTTACATCGCCGGCCCGGTCGCCAAAGCGATTTCCCAAGGCGCTC
TTGAGAATCCCGCAGTACTGCAAACACATGGTGGTAGAGCTCGGGCGATCGAGTCCGGTGAACTGCATATCGACGTCGCC
TTTGTAGCAGCGCCCGCGGCGGATGCCCACGGAAACATAAACGGCCTCGACGGCAAAGCTGCATGCGGCCCTCTCGGATA
TCCCATGGTCGATGCGCGCCATGCGGGTCACGTTATCGCGATCACGGACACCTTTTTCCCGACGCCGCTGGAGAACATCG
ACATCCTCGCAGGCCTCGTCGATGCCGTTGTCGTCGTGGATTCGATCGGCGACGCCTCGAAGATCGTCTCGGGGACAACA
CGTCTAACGACCGATCCTGTAGGTTTGTCGATCGCTGAAATGGCAGCCAGGGTGATCGAAGCATCGGGCCTTCTGATCGA
TGGCTTCTCGTTTCAGGCAGGTGCCGGGAGTGTCTCTCTGGCCGTTGCGGAGAACCTCGCCACGATTATGCGCGAGCGAG
GCATCGTTGGTGAATTCGCGTCAGGTGGCATCACCGGCGGGTTGGTCGAAATGTTTCGGGAAGGACTTTTCCGTACCCTC
TTTAATGTTCAGTCCTTCGATCTCGATGCCGTGACCTCCTACCGTGATGATCACGACCACTTTTCCATGTCGGCGTCGTT
ATACGCCAATCCGTCGCTGGGGAACGCTATCGTCAACAGGCTCGATGTCGTTGTATTGGGGGCTGCGGAGGTCGGCGTCA
ACTTCGACGTCAACGTGACAGCGGTAAACGGTGGTTCCATCATCGGCGGCTCGGGCGGGCATTCCGATACAGCAGCGGGA
GCGAAACTCGCCCTCGTAACGACGCGCCTTGTTTCTGGGAATAATCCGAAGATTGTGGATACCGTTGAATGCCGGACCAC
ACCTGGGGAAACGATAGATGTCGTCGTGACCGATTGCGGCGTAGCGGTAAATCCGCTGCGCTCCGACCTGAAGGCTAAAC
TACAGGCGGCGAACATTCCGGTCGTGGATATCGCAAGGCTTCGTGACCTTGCGGCTGAACAGACGAAAGACACGAAACGG
CCTCACTGTAATGCGCAAGGCAAGATCGTCGCAGTCGTAGAGTATCGAGACGGAAGCGTCATCGACGTGGTCCGGGCAAA
GGCCTGA

Upstream 100 bases:

>100_bases
CTCGCGATCGAGCCGCGCCTCAAAATCCTTCGCTTATCATAGCAGGGCGTATGGTTGCCCGACATCTGAAACCGTGGTTC
CGCCTATGAAGGGTAACGCG

Downstream 100 bases:

>100_bases
GCTCGTTACTCTACTTCGCCGCAGCCGCGTCCCTCTTTGCCTCCTCGAGACATCTTATGAAGTTGCGCACGGATGGCGTC
ATAAGGAGGTCCGGCCCCTG

Product: citrate lyase subunit alpha

Products: NA

Alternate protein names: Citrase alpha chain; Citrate (pro-3S)-lyase alpha chain; Citrate CoA-transferase subunit [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MVPIPSVIPGYGPVRAFVSAALATPEAKGANLPERRNGGHDKRAASLRDAIIASNLRNGGTVSFHHHLRDGDDVLRKVMT
EIAELGLRDLRVAATSIFPVHAPLVDYIRSGVVSRIVSSYIAGPVAKAISQGALENPAVLQTHGGRARAIESGELHIDVA
FVAAPAADAHGNINGLDGKAACGPLGYPMVDARHAGHVIAITDTFFPTPLENIDILAGLVDAVVVVDSIGDASKIVSGTT
RLTTDPVGLSIAEMAARVIEASGLLIDGFSFQAGAGSVSLAVAENLATIMRERGIVGEFASGGITGGLVEMFREGLFRTL
FNVQSFDLDAVTSYRDDHDHFSMSASLYANPSLGNAIVNRLDVVVLGAAEVGVNFDVNVTAVNGGSIIGGSGGHSDTAAG
AKLALVTTRLVSGNNPKIVDTVECRTTPGETIDVVVTDCGVAVNPLRSDLKAKLQAANIPVVDIARLRDLAAEQTKDTKR
PHCNAQGKIVAVVEYRDGSVIDVVRAKA

Sequences:

>Translated_508_residues
MVPIPSVIPGYGPVRAFVSAALATPEAKGANLPERRNGGHDKRAASLRDAIIASNLRNGGTVSFHHHLRDGDDVLRKVMT
EIAELGLRDLRVAATSIFPVHAPLVDYIRSGVVSRIVSSYIAGPVAKAISQGALENPAVLQTHGGRARAIESGELHIDVA
FVAAPAADAHGNINGLDGKAACGPLGYPMVDARHAGHVIAITDTFFPTPLENIDILAGLVDAVVVVDSIGDASKIVSGTT
RLTTDPVGLSIAEMAARVIEASGLLIDGFSFQAGAGSVSLAVAENLATIMRERGIVGEFASGGITGGLVEMFREGLFRTL
FNVQSFDLDAVTSYRDDHDHFSMSASLYANPSLGNAIVNRLDVVVLGAAEVGVNFDVNVTAVNGGSIIGGSGGHSDTAAG
AKLALVTTRLVSGNNPKIVDTVECRTTPGETIDVVVTDCGVAVNPLRSDLKAKLQAANIPVVDIARLRDLAAEQTKDTKR
PHCNAQGKIVAVVEYRDGSVIDVVRAKA
>Mature_508_residues
MVPIPSVIPGYGPVRAFVSAALATPEAKGANLPERRNGGHDKRAASLRDAIIASNLRNGGTVSFHHHLRDGDDVLRKVMT
EIAELGLRDLRVAATSIFPVHAPLVDYIRSGVVSRIVSSYIAGPVAKAISQGALENPAVLQTHGGRARAIESGELHIDVA
FVAAPAADAHGNINGLDGKAACGPLGYPMVDARHAGHVIAITDTFFPTPLENIDILAGLVDAVVVVDSIGDASKIVSGTT
RLTTDPVGLSIAEMAARVIEASGLLIDGFSFQAGAGSVSLAVAENLATIMRERGIVGEFASGGITGGLVEMFREGLFRTL
FNVQSFDLDAVTSYRDDHDHFSMSASLYANPSLGNAIVNRLDVVVLGAAEVGVNFDVNVTAVNGGSIIGGSGGHSDTAAG
AKLALVTTRLVSGNNPKIVDTVECRTTPGETIDVVVTDCGVAVNPLRSDLKAKLQAANIPVVDIARLRDLAAEQTKDTKR
PHCNAQGKIVAVVEYRDGSVIDVVRAKA

Specific function: Represents a citrate:acetyl-ACP transferase [H]

COG id: COG3051

COG function: function code C; Citrate lyase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786832, Length=463, Percent_Identity=46.8682505399568, Blast_Score=388, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006472 [H]

Pfam domain/function: PF04223 CitF [H]

EC number: =4.1.3.6; =2.8.3.10 [H]

Molecular weight: Translated: 52794; Mature: 52794

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPIPSVIPGYGPVRAFVSAALATPEAKGANLPERRNGGHDKRAASLRDAIIASNLRNGG
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCC
TVSFHHHLRDGDDVLRKVMTEIAELGLRDLRVAATSIFPVHAPLVDYIRSGVVSRIVSSY
EEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHH
IAGPVAKAISQGALENPAVLQTHGGRARAIESGELHIDVAFVAAPAADAHGNINGLDGKA
HHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCC
ACGPLGYPMVDARHAGHVIAITDTFFPTPLENIDILAGLVDAVVVVDSIGDASKIVSGTT
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
RLTTDPVGLSIAEMAARVIEASGLLIDGFSFQAGAGSVSLAVAENLATIMRERGIVGEFA
EEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCC
SGGITGGLVEMFREGLFRTLFNVQSFDLDAVTSYRDDHDHFSMSASLYANPSLGNAIVNR
CCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCEEEEEEEEECCCHHHHHHHH
LDVVVLGAAEVGVNFDVNVTAVNGGSIIGGSGGHSDTAAGAKLALVTTRLVSGNNPKIVD
EEEEEEEEHHHCCCEEEEEEEECCCEEECCCCCCCCCCCCCEEEEEEEEECCCCCCCEEE
TVECRTTPGETIDVVVTDCGVAVNPLRSDLKAKLQAANIPVVDIARLRDLAAEQTKDTKR
EEEECCCCCCEEEEEEECCCCEEHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCC
PHCNAQGKIVAVVEYRDGSVIDVVRAKA
CCCCCCCCEEEEEEECCCCEEEEEECCC
>Mature Secondary Structure
MVPIPSVIPGYGPVRAFVSAALATPEAKGANLPERRNGGHDKRAASLRDAIIASNLRNGG
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCC
TVSFHHHLRDGDDVLRKVMTEIAELGLRDLRVAATSIFPVHAPLVDYIRSGVVSRIVSSY
EEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHH
IAGPVAKAISQGALENPAVLQTHGGRARAIESGELHIDVAFVAAPAADAHGNINGLDGKA
HHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCC
ACGPLGYPMVDARHAGHVIAITDTFFPTPLENIDILAGLVDAVVVVDSIGDASKIVSGTT
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
RLTTDPVGLSIAEMAARVIEASGLLIDGFSFQAGAGSVSLAVAENLATIMRERGIVGEFA
EEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCC
SGGITGGLVEMFREGLFRTLFNVQSFDLDAVTSYRDDHDHFSMSASLYANPSLGNAIVNR
CCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCEEEEEEEEECCCHHHHHHHH
LDVVVLGAAEVGVNFDVNVTAVNGGSIIGGSGGHSDTAAGAKLALVTTRLVSGNNPKIVD
EEEEEEEEHHHCCCEEEEEEEECCCEEECCCCCCCCCCCCCEEEEEEEEECCCCCCCEEE
TVECRTTPGETIDVVVTDCGVAVNPLRSDLKAKLQAANIPVVDIARLRDLAAEQTKDTKR
EEEECCCCCCEEEEEEECCCCEEHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCC
PHCNAQGKIVAVVEYRDGSVIDVVRAKA
CCCCCCCCEEEEEEECCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7830578; 773936 [H]