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The map label for this gene is dctD [H]

Identifier: 209550299

GI number: 209550299

Start: 2766358

End: 2767704

Strand: Direct

Name: dctD [H]

Synonym: Rleg2_2719

Alternate gene names: 209550299

Gene position: 2766358-2767704 (Clockwise)

Preceding gene: 209550298

Following gene: 209550301

Centisome position: 60.96

GC content: 63.92

Gene sequence:

>1347_bases
ATGGCCACACCGATGCCCGTTGCGCTGATCGACGACGACAAGGATTTGCGCCGCGCCACCGCCCAGACGCTCGAACTCGC
CGGATTCTCGGTTTTCGCCTATGAGGGAGCGAAGGCCGCGCTTGCCGACCTTTCAGCCGACTTTGCCGGCCCCATCGTCA
CCGATATCCGCATGCCGGAGATCGACGGGCTGCAGTTGTTCGCCATGCTCAAGGGCATGGACGCCGACCTGCCGGTGATC
CTGATGACCGGTCATGGCGATATTCCGATGGCGGTTCAGGCAATCCAGGACGGCGCCTATGATTTCATTGCCAAACCTTT
TGCCGCCGACCGGCTCGTCCAGAGCGTGCGTCGCGCCAGCGAGAAGCGGCGGCTAATCCTGGAAAACCGCTTGCTGCGCA
AAGCTGCCGAGGATGCGCAGGAGAATTCACCGTTGATCGGCCAGACGCCGGTCATGGAAAATTTGCGGAAGATTCTTCGC
CATATCGCCGATACCGATGTCGACGTGCTCGTTGCCGGCGAAACCGGCAGCGGTAAGGAAGTCGTCGCCCAGATCCTGCA
TCAGTGGAGCCACCGCCGGAAGGGCAATTTCGTGGCGCTGAACTGCGGCGCGCTCCCCGAAACCGTCATCGAAAGCGAGC
TGTTCGGCCACGAGGCCGGCGCCTTTACCGGCGCCCAGAAGCGCCGCACCGGCCGCATCGAACATGCAAGCGGCGGCACG
CTTTTCCTCGACGAGATCGAGAGCATGCCGGTTGCCACCCAGGTCAAGATGCTGCGGGTGCTGGAGATGCGTGAAATCAC
CCCGCTCGGCACCAATGAAGTACGCCCGGTCGATCTGCGCGTCGTCGCAGCCGCCAAGATCGATCTCGGCGATCCCGACC
TGCGCGGCGATTTTCGCGAGGACCTCTATTACAGGCTGAATGTGGTGACGATCTCCATTCCGCCGCTGAGGGAGCGCCGC
GACGATATTCCGCTGCTGTTTTCCCATTTCGCCGCCCGCGCCGCCGAGCGCTTCCGCCGCGATGTTCCGGAGCTTTCGCC
CGAGGTGCGCCGCCACTTTGCCGCGCATGCATGGCCGGGCAATGTCCGCGAGCTCTCGCATTATGCCGAACGCGTGGTCC
TGGGTGTCGAAGGCGGCGCAGCCGCACCCGTCCCTGCCCAGCCGACCGGCGGCACACTGCCCGAACGGCTTGAGCGTTAC
GAGGCGGAGATCATCCGCGACGCGCTCACGGCCAATGACGGCGACGTGCGCAGCACCATCGAGGCGCTCGGCATTCCGAG
AAAGACGTTTTACGACAAGCTCCAGCGCCACGGCATCAACCGCGGCGGCTATTCCGCGCGCAAATAA

Upstream 100 bases:

>100_bases
ATCTCCAAGGACATCGTCGGCGATTACGGCGGGCGGATGGAGGTTGACAGCGACGGCGGCGGGACCCGGTTCGTCGTTCA
TCTGAGGAAGGCTTGAGATC

Downstream 100 bases:

>100_bases
CGCATGTCGCGCAAAAGTGTGCAGCGGTTTTGCGGCAACGACATGCGTAAAAAACCTAAAGCGCAACGAGCGAATGTGAA
AGATCGCGACGCGCTTTAGA

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MATPMPVALIDDDKDLRRATAQTLELAGFSVFAYEGAKAALADLSADFAGPIVTDIRMPEIDGLQLFAMLKGMDADLPVI
LMTGHGDIPMAVQAIQDGAYDFIAKPFAADRLVQSVRRASEKRRLILENRLLRKAAEDAQENSPLIGQTPVMENLRKILR
HIADTDVDVLVAGETGSGKEVVAQILHQWSHRRKGNFVALNCGALPETVIESELFGHEAGAFTGAQKRRTGRIEHASGGT
LFLDEIESMPVATQVKMLRVLEMREITPLGTNEVRPVDLRVVAAAKIDLGDPDLRGDFREDLYYRLNVVTISIPPLRERR
DDIPLLFSHFAARAAERFRRDVPELSPEVRRHFAAHAWPGNVRELSHYAERVVLGVEGGAAAPVPAQPTGGTLPERLERY
EAEIIRDALTANDGDVRSTIEALGIPRKTFYDKLQRHGINRGGYSARK

Sequences:

>Translated_448_residues
MATPMPVALIDDDKDLRRATAQTLELAGFSVFAYEGAKAALADLSADFAGPIVTDIRMPEIDGLQLFAMLKGMDADLPVI
LMTGHGDIPMAVQAIQDGAYDFIAKPFAADRLVQSVRRASEKRRLILENRLLRKAAEDAQENSPLIGQTPVMENLRKILR
HIADTDVDVLVAGETGSGKEVVAQILHQWSHRRKGNFVALNCGALPETVIESELFGHEAGAFTGAQKRRTGRIEHASGGT
LFLDEIESMPVATQVKMLRVLEMREITPLGTNEVRPVDLRVVAAAKIDLGDPDLRGDFREDLYYRLNVVTISIPPLRERR
DDIPLLFSHFAARAAERFRRDVPELSPEVRRHFAAHAWPGNVRELSHYAERVVLGVEGGAAAPVPAQPTGGTLPERLERY
EAEIIRDALTANDGDVRSTIEALGIPRKTFYDKLQRHGINRGGYSARK
>Mature_447_residues
ATPMPVALIDDDKDLRRATAQTLELAGFSVFAYEGAKAALADLSADFAGPIVTDIRMPEIDGLQLFAMLKGMDADLPVIL
MTGHGDIPMAVQAIQDGAYDFIAKPFAADRLVQSVRRASEKRRLILENRLLRKAAEDAQENSPLIGQTPVMENLRKILRH
IADTDVDVLVAGETGSGKEVVAQILHQWSHRRKGNFVALNCGALPETVIESELFGHEAGAFTGAQKRRTGRIEHASGGTL
FLDEIESMPVATQVKMLRVLEMREITPLGTNEVRPVDLRVVAAAKIDLGDPDLRGDFREDLYYRLNVVTISIPPLRERRD
DIPLLFSHFAARAAERFRRDVPELSPEVRRHFAAHAWPGNVRELSHYAERVVLGVEGGAAAPVPAQPTGGTLPERLERYE
AEIIRDALTANDGDVRSTIEALGIPRKTFYDKLQRHGINRGGYSARK

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=454, Percent_Identity=38.3259911894273, Blast_Score=318, Evalue=3e-88,
Organism=Escherichia coli, GI1788905, Length=448, Percent_Identity=38.8392857142857, Blast_Score=304, Evalue=7e-84,
Organism=Escherichia coli, GI1790437, Length=447, Percent_Identity=36.9127516778523, Blast_Score=285, Evalue=5e-78,
Organism=Escherichia coli, GI1790299, Length=375, Percent_Identity=40.8, Blast_Score=277, Evalue=8e-76,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=39.6875, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1789087, Length=334, Percent_Identity=38.622754491018, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI87082117, Length=320, Percent_Identity=41.25, Blast_Score=221, Evalue=5e-59,
Organism=Escherichia coli, GI87082152, Length=302, Percent_Identity=41.3907284768212, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1786524, Length=343, Percent_Identity=36.734693877551, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI87081872, Length=325, Percent_Identity=36.3076923076923, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1787583, Length=326, Percent_Identity=32.5153374233129, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI87081858, Length=301, Percent_Identity=31.5614617940199, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789828, Length=220, Percent_Identity=36.3636363636364, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49424; Mature: 49293

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPMPVALIDDDKDLRRATAQTLELAGFSVFAYEGAKAALADLSADFAGPIVTDIRMPE
CCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCEECCCCCC
IDGLQLFAMLKGMDADLPVILMTGHGDIPMAVQAIQDGAYDFIAKPFAADRLVQSVRRAS
CCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHH
EKRRLILENRLLRKAAEDAQENSPLIGQTPVMENLRKILRHIADTDVDVLVAGETGSGKE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
VVAQILHQWSHRRKGNFVALNCGALPETVIESELFGHEAGAFTGAQKRRTGRIEHASGGT
HHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHCCCCEECCCCE
LFLDEIESMPVATQVKMLRVLEMREITPLGTNEVRPVDLRVVAAAKIDLGDPDLRGDFRE
EEHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCHHH
DLYYRLNVVTISIPPLRERRDDIPLLFSHFAARAAERFRRDVPELSPEVRRHFAAHAWPG
CCEEEEEEEEEECCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCC
NVRELSHYAERVVLGVEGGAAAPVPAQPTGGTLPERLERYEAEIIRDALTANDGDVRSTI
CHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHH
EALGIPRKTFYDKLQRHGINRGGYSARK
HHHCCCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ATPMPVALIDDDKDLRRATAQTLELAGFSVFAYEGAKAALADLSADFAGPIVTDIRMPE
CCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCEECCCCCC
IDGLQLFAMLKGMDADLPVILMTGHGDIPMAVQAIQDGAYDFIAKPFAADRLVQSVRRAS
CCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHH
EKRRLILENRLLRKAAEDAQENSPLIGQTPVMENLRKILRHIADTDVDVLVAGETGSGKE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
VVAQILHQWSHRRKGNFVALNCGALPETVIESELFGHEAGAFTGAQKRRTGRIEHASGGT
HHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHCCCCEECCCCE
LFLDEIESMPVATQVKMLRVLEMREITPLGTNEVRPVDLRVVAAAKIDLGDPDLRGDFRE
EEHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCHHH
DLYYRLNVVTISIPPLRERRDDIPLLFSHFAARAAERFRRDVPELSPEVRRHFAAHAWPG
CCEEEEEEEEEECCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCC
NVRELSHYAERVVLGVEGGAAAPVPAQPTGGTLPERLERYEAEIIRDALTANDGDVRSTI
CHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHH
EALGIPRKTFYDKLQRHGINRGGYSARK
HHHCCCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3671068 [H]