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The map label for this gene is yjjG

Identifier: 209398693

GI number: 209398693

Start: 5540316

End: 5540993

Strand: Direct

Name: yjjG

Synonym: ECH74115_5886

Alternate gene names: 209398693

Gene position: 5540316-5540993 (Clockwise)

Preceding gene: 209399282

Following gene: 209399061

Centisome position: 99.43

GC content: 53.54

Gene sequence:

>678_bases
ATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTT
TCTTGATTACAGCGTCACCTTTACCGCTGAAGATTTTCAGGACTATCAGGCCGTTAACAAGCCACTGTGGGTGGATTATC
AAAACGGCGCGATCACTTCATTACAGCTTCAGCACGGGCGGTTTGAGAGCTGGGCCGAACGGCTGAACGTCGAGCCAGGT
AAACTCAACGAAGCCTTTATTAATGCGATGGCGGAAATCTGCACGCCGCTGCCGGGCGCGGTTTCTCTGCTTAACGCCAT
TCGTGGCAACGCCAAAATCGGCATCATCACCAACGGCTTTAGTGCCTTGCAACAGGTGCGTCTGGAACGCACGGGCCTGC
GTGATTACTTCGATTTGCTGGTGATTTCCGAAGAAGTTGGCGTTGCCAAACCGAATAAGAAAATTTTCGATTATGCGCTG
GAACAGGCGGGCAATCCTGACCGTTCACGCGTGCTGATGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAA
CGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTT
CGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGA

Upstream 100 bases:

>100_bases
ACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGT
ATGTAAGACAAGGTGGAATA

Downstream 100 bases:

>100_bases
TTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGA
AGAAAGAATTATGACGTTGT

Product: nucleotidase

Products: NA

Alternate protein names: House-cleaning nucleotidase; Non-canonical pyrimidine nucleotide phosphatase; Nucleoside 5'-monophosphate phosphohydrolase; dUMP phosphatase

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQAVNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPG
KLNEAFINAMAEICTPLPGAVSLLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHELEQLLCKH

Sequences:

>Translated_225_residues
MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQAVNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPG
KLNEAFINAMAEICTPLPGAVSLLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHELEQLLCKH
>Mature_225_residues
MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQAVNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPG
KLNEAFINAMAEICTPLPGAVSLLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHELEQLLCKH

Specific function: Nucleotidase that shows high phosphatase activity toward non-canonical pyrimidine nucleotides and three canonical nucleoside 5'-monophosphates (UMP, dUMP, and dTMP), and very low activity against TDP, IMP, UDP, GMP, dGMP, AMP, dAMP, and 6- phosphogluconat

COG id: COG1011

COG function: function code R; Predicted hydrolase (HAD superfamily)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. YjjG family

Homologues:

Organism=Homo sapiens, GI23308749, Length=245, Percent_Identity=30.6122448979592, Blast_Score=71, Evalue=9e-13,
Organism=Escherichia coli, GI1790833, Length=225, Percent_Identity=100, Blast_Score=469, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YJJG_ECO57 (P0A8Y2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D91295
- PIR:   F86136
- RefSeq:   NP_290987.1
- RefSeq:   NP_313359.1
- ProteinModelPortal:   P0A8Y2
- SMR:   P0A8Y2
- EnsemblBacteria:   EBESCT00000026251
- EnsemblBacteria:   EBESCT00000059873
- GeneID:   913547
- GeneID:   959681
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5975
- KEGG:   ecs:ECs5332
- GeneTree:   EBGT00050000011612
- HOGENOM:   HBG742904
- OMA:   FTELQTV
- ProtClustDB:   PRK09449
- BioCyc:   ECOL83334:ECS5332-MONOMER
- GO:   GO:0005737
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR011951
- Gene3D:   G3DSA:3.40.50.1000
- TIGRFAMs:   TIGR01549
- TIGRFAMs:   TIGR01509
- TIGRFAMs:   TIGR02254

Pfam domain/function: PF00702 Hydrolase; SSF56784 SSF56784

EC number: =3.1.3.5

Molecular weight: Translated: 25301; Mature: 25301

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: ACT_SITE 9-9

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQAVNKPLWVDYQNGAITS
CCCEEEEEECCCEEEEEHHHHHHHHHHHHEEEEEEHHHHHHHHHHCCCEEEEECCCCEEE
LQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVSLLNAIRGNAKIGIITNGF
EEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEECCH
SALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYALEQAGNPDRSRVLMVGDTAES
HHHHHHHHHHCCHHHHHHHHHEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCC
DILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHELEQLLCKH
HHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQAVNKPLWVDYQNGAITS
CCCEEEEEECCCEEEEEHHHHHHHHHHHHEEEEEEHHHHHHHHHHCCCEEEEECCCCEEE
LQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVSLLNAIRGNAKIGIITNGF
EEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEECCH
SALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYALEQAGNPDRSRVLMVGDTAES
HHHHHHHHHHCCHHHHHHHHHEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCC
DILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHELEQLLCKH
HHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796