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The map label for this gene is rimI

Identifier: 209399282

GI number: 209399282

Start: 5539855

End: 5540301

Strand: Direct

Name: rimI

Synonym: ECH74115_5885

Alternate gene names: 209399282

Gene position: 5539855-5540301 (Clockwise)

Preceding gene: 209397348

Following gene: 209398693

Centisome position: 99.42

GC content: 52.13

Gene sequence:

>447_bases
ATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTG
GAGTGAAAAAACCTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGT
TTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGCGTCAGGGATTG
GGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCCTC
AAATGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGG
ACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAA

Upstream 100 bases:

>100_bases
CCGCTATCACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGAATTACGGGCAAACCCAACGGCACGCGCCGCGTT
ATGGCAACAAATTTGCACAT

Downstream 100 bases:

>100_bases
GACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGC
CTGCAGCGGATGTTTCTTGA

Product: ribosomal-protein-alanine N-acetyltransferase

Products: NA

Alternate protein names: Acetylating enzyme for N-terminal of ribosomal protein S18

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQLTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGL
GRALLEHLIDELEKRGVATLWLEVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM

Sequences:

>Translated_148_residues
MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQLTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGL
GRALLEHLIDELEKRGVATLWLEVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM
>Mature_148_residues
MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQLTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGL
GRALLEHLIDELEKRGVATLWLEVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM

Specific function: This enzyme acetylates the N-terminal alanine of ribosomal protein S18

COG id: COG0456

COG function: function code R; Acetyltransferases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain

Homologues:

Organism=Escherichia coli, GI2367381, Length=148, Percent_Identity=100, Blast_Score=306, Evalue=5e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMI_ECO57 (P0A946)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C91295
- PIR:   E86136
- RefSeq:   NP_290986.1
- RefSeq:   NP_313358.1
- ProteinModelPortal:   P0A946
- SMR:   P0A946
- EnsemblBacteria:   EBESCT00000028511
- EnsemblBacteria:   EBESCT00000058815
- GeneID:   913548
- GeneID:   959682
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5974
- KEGG:   ecs:ECs5331
- GeneTree:   EBGT00050000011383
- HOGENOM:   HBG742014
- OMA:   RENAVVM
- ProtClustDB:   PRK09491
- BioCyc:   ECOL83334:ECS5331-MONOMER
- GO:   GO:0005737
- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR006464
- Gene3D:   G3DSA:3.40.630.30
- TIGRFAMs:   TIGR01575

Pfam domain/function: PF00583 Acetyltransf_1; SSF55729 Acyl_CoA_acyltransferase

EC number: =2.3.1.128

Molecular weight: Translated: 16610; Mature: 16610

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS51186 GNAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQLTQNGKMAAFAITQVV
CCCCCCCCCCCCCCCEEHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHH
LDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWLEVRASNAAAIALYESLGF
HCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCEEEEEEECCCC
NEATIRRNYYPTTDGREDAIIMALPISM
CCEEEECCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQLTQNGKMAAFAITQVV
CCCCCCCCCCCCCCCEEHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHH
LDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWLEVRASNAAAIALYESLGF
HCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCEEEEEEECCCC
NEATIRRNYYPTTDGREDAIIMALPISM
CCEEEECCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796