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The map label for this gene is prfC [H]

Identifier: 209399061

GI number: 209399061

Start: 5541084

End: 5542673

Strand: Direct

Name: prfC [H]

Synonym: ECH74115_5887

Alternate gene names: 209399061

Gene position: 5541084-5542673 (Clockwise)

Preceding gene: 209398693

Following gene: 209398467

Centisome position: 99.44

GC content: 53.96

Gene sequence:

>1590_bases
ATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACTTTTGCCATTATTTCTCACCCGGACGCCGGTAAGAC
TACCATCACCGAGAAGGTGCTGCTGTTCGGACAGGCCATTCAGACCGCCGGTACAGTAAAAGGCCGTGGTTCCAACCAGC
ACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCAC
GATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGA
CTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCG
ACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAATTGCTCGATGAAGTTGAGAAC
GAGCTGAAAATTGGCTGCGCACCGATCACCTGGCCAATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTACCACCTTTATAA
AGATGAAACCTATCTCTATCAGAGCGGTAAAGGGCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGG
ATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAG
TTCGACAAAGAGCTGTTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTTGATCA
TATGCTGGATGGCCTGGTGGAGTGGGCGCCTGCGCCGATGCCGCGTCAGACCGATACCCGTACCGTAGAAGCGAGCGAAG
ATAAATTTACCGGCTTCGTATTTAAAATTCAGGCCAACATGGACCCGAAACACCGCGACCGCGTGGCGTTTATGCGTGTG
GTGTCCGGTAAATATGAAAAAGGCATGAAGCTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGAC
CTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTC
AGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACTTCGCACCAGAACTGTTCCGTCGT
ATCCGCCTGAAAGATCCGCTGAAGCAAAAACAGCTGCTCAAAGGGCTGGTACAGCTTTCCGAAGAGGGCGCGGTGCAGGT
GTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCGGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGA
AGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGTTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAG
AAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCTACCAG
CATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAA

Upstream 100 bases:

>100_bases
TAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGC
TTACTCAAGAAGAAAGAATT

Downstream 100 bases:

>100_bases
TTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGTCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAA
CATTCACTTTTGCTTATGTT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYH
DCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELELVKGASNE
FDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAPAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRV
VSGKYEKGMKLRQVRTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNFAPELFRR
IRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVARLKSEYNVEAVYESVNVATARWVECADAK
KFEEFKRKNESQLALDGGDNLAYIATSMVNLRLAQERYPDVQFHQTREH

Sequences:

>Translated_529_residues
MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYH
DCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELELVKGASNE
FDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAPAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRV
VSGKYEKGMKLRQVRTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNFAPELFRR
IRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVARLKSEYNVEAVYESVNVATARWVECADAK
KFEEFKRKNESQLALDGGDNLAYIATSMVNLRLAQERYPDVQFHQTREH
>Mature_528_residues
TLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHD
CLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELELVKGASNEF
DKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAPAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVV
SGKYEKGMKLRQVRTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNFAPELFRRI
RLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVARLKSEYNVEAVYESVNVATARWVECADAKK
FEEFKRKNESQLALDGGDNLAYIATSMVNLRLAQERYPDVQFHQTREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=498, Percent_Identity=28.1124497991968, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI19923640, Length=511, Percent_Identity=26.4187866927593, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=28.1725888324873, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI25306287, Length=483, Percent_Identity=26.0869565217391, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI157426893, Length=169, Percent_Identity=28.4023668639053, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI94966754, Length=164, Percent_Identity=28.6585365853659, Blast_Score=76, Evalue=8e-14,
Organism=Escherichia coli, GI1790835, Length=529, Percent_Identity=100, Blast_Score=1101, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=493, Percent_Identity=25.9634888438134, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI48994988, Length=215, Percent_Identity=30.6976744186047, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=32.0512820512821, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17533571, Length=521, Percent_Identity=26.4875239923225, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17556745, Length=164, Percent_Identity=31.7073170731707, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=152, Percent_Identity=30.2631578947368, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=479, Percent_Identity=28.1837160751566, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=423, Percent_Identity=26.4775413711584, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323320, Length=157, Percent_Identity=24.8407643312102, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324707, Length=157, Percent_Identity=26.1146496815287, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320593, Length=157, Percent_Identity=26.1146496815287, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24582462, Length=494, Percent_Identity=26.1133603238866, Blast_Score=166, Evalue=5e-41,
Organism=Drosophila melanogaster, GI221458488, Length=521, Percent_Identity=24.3761996161228, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=32.7044025157233, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=29.2207792207792, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=29.2207792207792, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=29.2207792207792, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59575; Mature: 59444

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSNQHAKSDWM
CCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
EMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVE
HHHHHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH
DRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENELKIGCAPITWPIGCGKLFK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHH
GVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELELVKGASNE
HHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
FDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAPAPMPRQTDTRTVEASEDKFTGFV
CCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCEEEEE
FKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVRTAKDVVISDALTFMAGDRSHVEEAY
EEEEECCCCCHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCC
PGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNFAPELFRRIRLKDPLKQKQLLKGLVQLS
CCCEEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHC
EEGAVQVFRPISNNDLIVGAVGVLQFDVVVARLKSEYNVEAVYESVNVATARWVECADAK
CCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
KFEEFKRKNESQLALDGGDNLAYIATSMVNLRLAQERYPDVQFHQTREH
HHHHHHCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSNQHAKSDWM
CCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
EMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVE
HHHHHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH
DRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENELKIGCAPITWPIGCGKLFK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHH
GVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELELVKGASNE
HHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
FDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAPAPMPRQTDTRTVEASEDKFTGFV
CCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCEEEEE
FKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVRTAKDVVISDALTFMAGDRSHVEEAY
EEEEECCCCCHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCC
PGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNFAPELFRRIRLKDPLKQKQLLKGLVQLS
CCCEEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHC
EEGAVQVFRPISNNDLIVGAVGVLQFDVVVARLKSEYNVEAVYESVNVATARWVECADAK
CCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
KFEEFKRKNESQLALDGGDNLAYIATSMVNLRLAQERYPDVQFHQTREH
HHHHHHCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA