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The map label for this gene is gne

Identifier: 209398311

GI number: 209398311

Start: 2753564

End: 2754559

Strand: Reverse

Name: gne

Synonym: ECH74115_2976

Alternate gene names: 209398311

Gene position: 2754559-2753564 (Counterclockwise)

Preceding gene: 209397395

Following gene: 209400008

Centisome position: 49.44

GC content: 45.48

Gene sequence:

>996_bases
ATGAACGATAACGTTTTGCTCATAGGAGCTTCCGGATTCGTAGGAACCCGACTACTTGAAACGGCAATTGCTGACTTTAA
TATCAAGAACCTGGACAAACAGCAGAGCCACTTTTATCCAGAAATCACACAGATTGGTGATGTTCGTGATCAACAGGCAC
TCGACCAGGCGTTAGCCGGTTTTGACACTGTTGTACTACTGGCAGCGGAACACCGCGATGACGTCAGCCCTACTTCTCTC
TATTATGATGTCAACGTTCAGGGTACCCGCAATGTGCTGGCGGCCATGGAAAAAAATGGCGTTAAAAATATCATCTTTAC
CAGTTCCGTTGCTGTTTATGGTTTGAACAAACACAACCCTGACGAAAACCATCCACACGACCCTTTCAACCACTACGGCA
AAAGCAAGTGGCAGGCGGAGGAAGTGCTGCGTGAATGGTATAACAAAGCACCAACAGAACGTTCATTAACTATCATCCGT
CCTACCGTTATCTTCGGTGAACGCAACCGCGGTAACGTCTATAACTTGCTGAAACAGATCGCTGGCGGCAAGTTTATGAT
GGTGGGCGCAGGGACTAACTATAAGTCCATGGCTTATGTTGGAAACATTGTTGAGTTTATCAAGTACAAACTGAAGAATG
TTGCCGCAGGTTACGAGGTTTATAACTACGTTGATAAGCCAGACCTGAACATGAACCAGTTGGTTGCTGAAGTTGAACAA
AGCCTGAACAAAAAGATCCCTTCTATGCACTTGCCTTACCCACTAGGAATGCTGGGTGGATATTGCTTTGATATCCTGAG
CAAAATTACGGGCAAAAAATACGCTGTCAGCTCTGTGCGCGTGAAAAAATTCTGCGCAACAACACAGTTTGACGCAACGA
AAGTGCATTCTTCAGGTTTTGTGGCACCGTATACGCTGTCGCAAGGTCTGGATCGAACTCTGCAGTATGAATTCGTCCAT
GCCAAAAAAGACGACATAACGTTTGTTTCTGAGTAA

Upstream 100 bases:

>100_bases
CACTAACTGTTAAAGCTGGGGCTGGGCAGCATTGACTACTCAGGGAAGGTACTCATCTTTATCTTGTGTTTCTTACGTAA
CAACCAGACTAAAGTGATTT

Downstream 100 bases:

>100_bases
TGCAGACGAGTCAGAGAAAAAAGGACCAGGTTGGACTACGATTATTCGGAAAGAGGTACTGATTAAATTATCAGTATGTG
GATTTCCGAGTAATCCGAGT

Product: UDP-N-acetylglucosamine 4-epimerase

Products: NA

Alternate protein names: UDP-GlcNAc 4-epimerase

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQIGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSL
YYDVNVQGTRNVLAAMEKNGVKNIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGYEVYNYVDKPDLNMNQLVAEVEQ
SLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAVSSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVH
AKKDDITFVSE

Sequences:

>Translated_331_residues
MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQIGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSL
YYDVNVQGTRNVLAAMEKNGVKNIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGYEVYNYVDKPDLNMNQLVAEVEQ
SLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAVSSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVH
AKKDDITFVSE
>Mature_331_residues
MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQIGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSL
YYDVNVQGTRNVLAAMEKNGVKNIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGYEVYNYVDKPDLNMNQLVAEVEQ
SLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAVSSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVH
AKKDDITFVSE

Specific function: Converts UDP-GlcNAc to UDP-GalNAc. Essential for O antigen synthesis

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family

Homologues:

Organism=Homo sapiens, GI193211614, Length=262, Percent_Identity=28.2442748091603, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI8393516, Length=262, Percent_Identity=28.2442748091603, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI56237023, Length=294, Percent_Identity=28.2312925170068, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI56118217, Length=294, Percent_Identity=28.2312925170068, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI189083684, Length=294, Percent_Identity=28.2312925170068, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1786974, Length=299, Percent_Identity=30.4347826086957, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081792, Length=307, Percent_Identity=24.7557003257329, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321437, Length=345, Percent_Identity=26.3768115942029, Blast_Score=92, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GNE_ECO57 (Q8X7P7)

Other databases:

- EMBL:   AF461121
- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B85830
- PIR:   G90984
- RefSeq:   NP_288548.1
- RefSeq:   NP_310874.1
- ProteinModelPortal:   Q8X7P7
- SMR:   Q8X7P7
- EnsemblBacteria:   EBESCT00000028936
- EnsemblBacteria:   EBESCT00000058570
- GeneID:   912320
- GeneID:   962101
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3206
- KEGG:   ecs:ECs2847
- GeneTree:   EBGT00050000008808
- HOGENOM:   HBG391172
- OMA:   QKAKSED
- ProtClustDB:   CLSK880278
- BioCyc:   ECOL83334:ECS2847-MONOMER
- InterPro:   IPR001509
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF01370 Epimerase

EC number: =5.1.3.7

Molecular weight: Translated: 37143; Mature: 37143

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: ACT_SITE 132-132 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQIGDVRDQQALDQALAG
CCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHCCCHHHHHHHHHHHC
FDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVKNIIFTSSVAVYGLNKHNP
CCEEEEEEECCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEECCEEEEECCCCCC
DENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIRPTVIFGERNRGNVYNLLKQI
CCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHH
AGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGYEVYNYVDKPDLNMNQLVAEVEQ
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
SLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAVSSVRVKKFCATTQFDATKVHSSGF
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCHHEECCCCC
VAPYTLSQGLDRTLQYEFVHAKKDDITFVSE
CCCHHHHHCCCHHEEEEEEECCCCCEEEECC
>Mature Secondary Structure
MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQIGDVRDQQALDQALAG
CCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHCCCHHHHHHHHHHHC
FDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVKNIIFTSSVAVYGLNKHNP
CCEEEEEEECCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEECCEEEEECCCCCC
DENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIRPTVIFGERNRGNVYNLLKQI
CCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHH
AGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGYEVYNYVDKPDLNMNQLVAEVEQ
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
SLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAVSSVRVKKFCATTQFDATKVHSSGF
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCHHEECCCCC
VAPYTLSQGLDRTLQYEFVHAKKDDITFVSE
CCCHHHHHCCCHHEEEEEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11976290; 11206551; 11258796