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The map label for this gene is wcaM [H]

Identifier: 209397395

GI number: 209397395

Start: 2754717

End: 2756111

Strand: Reverse

Name: wcaM [H]

Synonym: ECH74115_2977

Alternate gene names: 209397395

Gene position: 2756111-2754717 (Counterclockwise)

Preceding gene: 209399030

Following gene: 209398311

Centisome position: 49.46

GC content: 47.17

Gene sequence:

>1395_bases
ATGCCATTTAAAAAACTCTCCCGACGCACCTTCCTGACGGCAAGCTCGGCGCTTGCCTTCCTTCATACCCCTTTCGCTCG
CGCATTTCCCGCCCGACAAAGCGTTAATATCAACGACTACAACCCTCACGACTGGATCGCCTCATTTAAACAAGCCTTCA
GCGAAGGGCAAACGGTTGTCGTGCCTGCTGGATTCGTTTGTGACAATATCAACACCGGCATCTTCATTCCTCCTGGCAAA
ACGTTACACATCCTTGGAAGCCTGCGCGGCAACGGCAGAGGGCGATTTGTCTTACAGGACGGCAGCCAGGTGACAGGGGA
GGAGGGCGGCAGTATGCATAACATCACCCTGGATGTGCGTGGTTCTGACTGCACCATCAAAGGGCTGGCGATGAGCGGCT
TTGGTCCGGTAACGCAGATTTATATCGGCGGCAAAAACAAACGGGTCATGCGCAACCTGACCATCGATAACCTGACCATT
AGCCACGCTAATTACGCCATCTTACGCCAGGGATTTCATAACCAGATTATCGGTGCCAACATCACCAATTGTAAGTTCAG
CGACTTACAGGGCGATGCCATTGAATGGAACGTGGCGATTAACGACCGCGATATTTTGATCTCCGACCATGTCATCGAGC
GCATCAACTGTACTAACGGCAAAATCAACTGGGGCATTGGCATAGGCCTTGCGGGAAGCACTTACGATAATAATTACCCG
GAAGATCAGGCAGTGAAAAACTTTGTCGTGGCGAATATCACGGGATCGGATTGTCGGCAGTTGATACATGTTGAAAATGG
TAAACATTTTGTTATTCGTAATATCAAAGCCCGCAATATCACGCAGGATTTCAGTAAGAAAGCAGGTATTGATAACGCCA
CAGTCGCTATTTACGGTTGTGACAATTTCGTGATTGATAATATTGAAATGATTAATAGCGCCGGGATGTTAATCGGCTAT
GGGGTAATTAAAGGCAATTATCTCTCGATTCCGCAAAATTTCCGAGTGAATAATATTCAACTGGATAACACCCATCTTGC
TTATAAATTGCGCGGCCTCCAAATCTCTGCCGGGAATGCCGTCTCCTTTGTGGCACTAACTAACATTGAGATGAAGCGTG
CGTCGCTGGAGTTACACAACAAACCGCAACATCTTTTTCTGCGTAATATCAAAGTGATGCAGGAATCCTCTGTTGGACCC
GCATTGATTATGAACTTCGACATGCGCAAAGACGTTCGAGGCGTCTTTATGGCGAAAGAAGAAACACTGCTGTCTCTTGC
AAATGTTCATGCGGTGAATGAGAAAGGACAAAGCTCCGTCGATATCGACAGGATTAATCACCATATTGTTAATGTGGAAA
AGATTAACTTTAGATTGCCGGAACGGAGAGAGTAG

Upstream 100 bases:

>100_bases
GTCGTCAAACGCGCGCGCGAAAAAGTTGAACACGATTTTAACCAGCAGGTGATCAATCGAGAACTCGCCAGCTTGCTGCA
GGCTTTATAGAGGAGAACGT

Downstream 100 bases:

>100_bases
ATTTGCGACCATTCATAAACTTACTGGATATAATGTTCTAAAAGATTATAATCCTCACACTAACTGTTAAAGCTGGGGCT
GGGCAGCATTGACTACTCAG

Product: putative colanic acid biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MPFKKLSRRTFLTASSALAFLHTPFARAFPARQSVNINDYNPHDWIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGK
TLHILGSLRGNGRGRFVLQDGSQVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTI
SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYP
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITQDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGY
GVIKGNYLSIPQNFRVNNIQLDNTHLAYKLRGLQISAGNAVSFVALTNIEMKRASLELHNKPQHLFLRNIKVMQESSVGP
ALIMNFDMRKDVRGVFMAKEETLLSLANVHAVNEKGQSSVDIDRINHHIVNVEKINFRLPERRE

Sequences:

>Translated_464_residues
MPFKKLSRRTFLTASSALAFLHTPFARAFPARQSVNINDYNPHDWIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGK
TLHILGSLRGNGRGRFVLQDGSQVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTI
SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYP
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITQDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGY
GVIKGNYLSIPQNFRVNNIQLDNTHLAYKLRGLQISAGNAVSFVALTNIEMKRASLELHNKPQHLFLRNIKVMQESSVGP
ALIMNFDMRKDVRGVFMAKEETLLSLANVHAVNEKGQSSVDIDRINHHIVNVEKINFRLPERRE
>Mature_463_residues
PFKKLSRRTFLTASSALAFLHTPFARAFPARQSVNINDYNPHDWIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKT
LHILGSLRGNGRGRFVLQDGSQVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTIS
HANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYPE
DQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITQDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYG
VIKGNYLSIPQNFRVNNIQLDNTHLAYKLRGLQISAGNAVSFVALTNIEMKRASLELHNKPQHLFLRNIKVMQESSVGPA
LIMNFDMRKDVRGVFMAKEETLLSLANVHAVNEKGQSSVDIDRINHHIVNVEKINFRLPERRE

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788356, Length=464, Percent_Identity=96.551724137931, Blast_Score=926, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011050 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 51419; Mature: 51287

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFKKLSRRTFLTASSALAFLHTPFARAFPARQSVNINDYNPHDWIASFKQAFSEGQTVV
CCHHHHCCEEEEEHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEE
VPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGSQVTGEEGGSMHNITLDVR
EECCEEECCCCCCEEECCCCEEEEEEECCCCCCEEEEEECCCEECCCCCCCEEEEEEEEE
GSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTISHANYAILRQGFHNQIIGAN
CCCEEEEEEEECCCCCEEEEEECCCCCEEEEEEEECEEEEECCCHHHHHCCCCCCCCCCC
ITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYP
CCCCEECCCCCCEEEEEEEECCCEEEEECHHHHEEECCCCEEEEEEEEEECCCCCCCCCC
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITQDFSKKAGIDNATVAIYGC
CHHHHCEEEEEECCCHHHHEEEEECCCCEEEEEECCCCCCHHHHHHHCCCCCCEEEEEEC
DNFVIDNIEMINSAGMLIGYGVIKGNYLSIPQNFRVNNIQLDNTHLAYKLRGLQISAGNA
CCEEECCEEEECCCCEEEEEEEEECCEEECCCCCEEEEEEECCCEEEEEEEEEEEECCCE
VSFVALTNIEMKRASLELHNKPQHLFLRNIKVMQESSVGPALIMNFDMRKDVRGVFMAKE
EEEEEEECEEEEEEEEEECCCCCEEEEEHEEEEECCCCCCEEEEEECCCCCCCEEEEECH
ETLLSLANVHAVNEKGQSSVDIDRINHHIVNVEKINFRLPERRE
HHHHHHHHEEEECCCCCCCCCHHHCCEEEEEEEEEEEECCCCCC
>Mature Secondary Structure 
PFKKLSRRTFLTASSALAFLHTPFARAFPARQSVNINDYNPHDWIASFKQAFSEGQTVV
CHHHHCCEEEEEHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEE
VPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGSQVTGEEGGSMHNITLDVR
EECCEEECCCCCCEEECCCCEEEEEEECCCCCCEEEEEECCCEECCCCCCCEEEEEEEEE
GSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTISHANYAILRQGFHNQIIGAN
CCCEEEEEEEECCCCCEEEEEECCCCCEEEEEEEECEEEEECCCHHHHHCCCCCCCCCCC
ITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYP
CCCCEECCCCCCEEEEEEEECCCEEEEECHHHHEEECCCCEEEEEEEEEECCCCCCCCCC
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITQDFSKKAGIDNATVAIYGC
CHHHHCEEEEEECCCHHHHEEEEECCCCEEEEEECCCCCCHHHHHHHCCCCCCEEEEEEC
DNFVIDNIEMINSAGMLIGYGVIKGNYLSIPQNFRVNNIQLDNTHLAYKLRGLQISAGNA
CCEEECCEEEECCCCEEEEEEEEECCEEECCCCCEEEEEEECCCEEEEEEEEEEEECCCE
VSFVALTNIEMKRASLELHNKPQHLFLRNIKVMQESSVGPALIMNFDMRKDVRGVFMAKE
EEEEEEECEEEEEEEEEECCCCCEEEEEHEEEEECCCCCCEEEEEECCCCCCCEEEEECH
ETLLSLANVHAVNEKGQSSVDIDRINHHIVNVEKINFRLPERRE
HHHHHHHHEEEECCCCCCCCCHHHCCEEEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]