Click here to switch to the map view.

The map label for this gene is galF

Identifier: 209400008

GI number: 209400008

Start: 2752428

End: 2753321

Strand: Reverse

Name: galF

Synonym: ECH74115_2975

Alternate gene names: 209400008

Gene position: 2753321-2752428 (Counterclockwise)

Preceding gene: 209398311

Following gene: 209400561

Centisome position: 49.41

GC content: 50.67

Gene sequence:

>894_bases
ATGACGAATTTAAAAGCAGTTATACCGGTAGCAGGTCTTGGGATGCATATGTTGCCTGCCACTAAGGCGATTCCCAAAGA
GATGCTACCGATCGTCGACAAGCCAATGATTCAGTACATTGTTGACGAGATTGTGGCTGCAGGGATCAAAGAAATCCTCC
TGGTAACTCACGCGTCCAAGAACGCAGTCGAAAACCACTTCGACACCTCTTATGAATTAGAATCTCTCCTTGAACAGCGC
GTGAAGCGTCAACTACTGGCGGAAGTACAATCTATCTGTCCGCCGGGCGTGACCATTATGAACGTGCGTCAGGGTGAACC
TTTAGGTTTAGGCCACTCCATTTTATGTGCGCGACCTGCCATTGGTGACAATCCATTTGTCGTGGTGCTGCCAGACGTTG
TGATCGACGACGCCAGCGCCGACCCGCTACGCTACAACCTTGCTGCCATGATTGCGCGCTTCAATGAAACGGGACGCAGC
CAGGTGCTGGCAAAACGTATGCCGGGCGACCTCTCTGAATACTCCGTCATCCAGACCAAAGAACCGCTGGATCGTGAAGG
CAAAGTCAGCCGCATTGTTGAATTTATCGAAAAGCCGGATCAGCCGCAGACGCTGGACTCAGACATCATGGCCGTTGGTC
GATATGTGCTTTCTGCCGATATTTGGCCGGAACTGGAACGTACTCAGCCTGGTGCATGGGGACGTATTCAGCTGACTGAT
GCCATTGCTGAGCTGGCAAAAAAACAATCCGTTGATGCAATGCTGATGACTGGCGACAGTTACGACTGCGGTAAAAAAAT
GGGCTATATGCAGGCGTTTGTGAAGTATGGACTACGCAACTTGAAAGAAGGGGCGAAGTTCCGCAAAGGTATCGAGAAGC
TGTTAAGCGAATAA

Upstream 100 bases:

>100_bases
TCAAAGTTATGCGGAACTAAGCTTTGGTATATAGCCATAACGTTTGGCTAACCTATGGCAATAATTTACGCACTATCAGC
CACTTAGAGGTAGGAAAAGA

Downstream 100 bases:

>100_bases
TGAAAATCTGACCGAATGTAACGGTTGATAAGGAAATAATATCGGCAGTGAAAAACTATAGATCAATAAATATCACTGCT
GTTGAGCTTTTATAATTAAC

Product: UTP--glucose-1-phosphate uridylyltransferase subunit GalF

Products: NA

Alternate protein names: Alpha-D-glucosyl-1-phosphate uridylyltransferase; UDP-glucose pyrophosphorylase; UDPGP; Uridine diphosphoglucose pyrophosphorylase

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQR
VKRQLLAEVQSICPPGVTIMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLSADIWPELERTQPGAWGRIQLTD
AIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKYGLRNLKEGAKFRKGIEKLLSE

Sequences:

>Translated_297_residues
MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQR
VKRQLLAEVQSICPPGVTIMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLSADIWPELERTQPGAWGRIQLTD
AIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKYGLRNLKEGAKFRKGIEKLLSE
>Mature_296_residues
TNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRV
KRQLLAEVQSICPPGVTIMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRSQ
VLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLSADIWPELERTQPGAWGRIQLTDA
IAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKYGLRNLKEGAKFRKGIEKLLSE

Specific function: Lipopolysaccharide biosynthesis. [C]

COG id: COG1210

COG function: function code M; UDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDPGP type 2 family

Homologues:

Organism=Escherichia coli, GI1788355, Length=297, Percent_Identity=100, Blast_Score=609, Evalue=1e-176,
Organism=Escherichia coli, GI1787488, Length=289, Percent_Identity=58.477508650519, Blast_Score=342, Evalue=1e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GALF_ECO57 (P0AAB7)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A85830
- PIR:   F90984
- RefSeq:   NP_288547.1
- RefSeq:   NP_310873.1
- ProteinModelPortal:   P0AAB7
- SMR:   P0AAB7
- EnsemblBacteria:   EBESCT00000027345
- EnsemblBacteria:   EBESCT00000059751
- GeneID:   914113
- GeneID:   962100
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3205
- KEGG:   ecs:ECs2846
- GeneTree:   EBGT00050000009639
- HOGENOM:   HBG688195
- OMA:   MDEIMTN
- ProtClustDB:   PRK10122
- BioCyc:   ECOL83334:ECS2846-MONOMER
- InterPro:   IPR005774
- InterPro:   IPR005835
- TIGRFAMs:   TIGR01105

Pfam domain/function: PF00483 NTP_transferase

EC number: =2.7.7.9

Molecular weight: Translated: 32830; Mature: 32699

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEIVAAGIKEILLVTHASK
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCH
NAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNVRQGEPLGLGHSILCARPA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHEEECCC
IGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRSQVLAKRMPGDLSEYSVIQTK
CCCCCEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC
EPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLSADIWPELERTQPGAWGRIQLTD
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEHHH
AIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKYGLRNLKEGAKFRKGIEKLLSE
HHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEIVAAGIKEILLVTHASK
CCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCH
NAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNVRQGEPLGLGHSILCARPA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHEEECCC
IGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRSQVLAKRMPGDLSEYSVIQTK
CCCCCEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC
EPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLSADIWPELERTQPGAWGRIQLTD
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEHHH
AIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKYGLRNLKEGAKFRKGIEKLLSE
HHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796