Click here to switch to the map view.

The map label for this gene is grpE

Identifier: 209695893

GI number: 209695893

Start: 2648330

End: 2648914

Strand: Reverse

Name: grpE

Synonym: VSAL_I2473

Alternate gene names: 209695893

Gene position: 2648914-2648330 (Counterclockwise)

Preceding gene: 209695897

Following gene: 209695892

Centisome position: 79.66

GC content: 40.17

Gene sequence:

>585_bases
ATGAATAACGAAGAAAAAAAAGTAAACGAAGAAATCGTAGTTGAAGATCAAATCGAAGCTATTGGTACTGAAGCCGATGT
TGAGTGGAACGAATCTATGGAAGAAAGCCAAGACGCTAAAATTGCAGAATTAGAAGCGGCATTACTTGCAAGCCAAGCGC
AGCTTAAAGAACAACAAGATGCGGTTTTACGAGCGAAAGCTGAAGAGCAAAATGTTCGTCGTCGTGCTGAAGGTGATATT
GATAAAGCGCGTAAATACGCACTGAAGAAATTTGCTGGTGAACTACTGCCTGTTATCGACAATCTAGAGCGTGCTCTAGA
AAGCGGAGATAAAGAAAACGAAGCGGCAAAAGTGTTGCTTGAAGGTGTTGAACTTACATTACAAACGTTTATTTCAACAA
TCGAAAAATTTGGTCTAACAGTGATTAACCCAGTGGGTGAAACCTTTAATCCTGAGCATCACCAAGCCATTGGTATGCAA
GCAAGTCCAGATCATGAATCAAACACCGTAATGGTTGTAATGCAGAAAGGCTACTCATTAAATGAGCAAGTTATTCGTCC
TGCAATGGTTATGGTTGCTCAATAA

Upstream 100 bases:

>100_bases
CCCATAATAATGACAAAGTTTTAATTTGCTTGTAGAGCGTCGCCTTGGTTTGTATTAAGATCGCTTTATGGCTAATCAGC
AGTCTTCTGGAGAAGCAAAA

Downstream 100 bases:

>100_bases
TCAATTTTTTTGATTTATTAAGTTAATTTAAATTAAGCGGTACCAGCGATGGTACCGCTTTTTTTGTCTCTGCTTTAAGA
TGCTAAGTCTTTGTTTTGGG

Product: protein GrpE (heat shock protein)

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 194; Mature: 194

Protein sequence:

>194_residues
MNNEEKKVNEEIVVEDQIEAIGTEADVEWNESMEESQDAKIAELEAALLASQAQLKEQQDAVLRAKAEEQNVRRRAEGDI
DKARKYALKKFAGELLPVIDNLERALESGDKENEAAKVLLEGVELTLQTFISTIEKFGLTVINPVGETFNPEHHQAIGMQ
ASPDHESNTVMVVMQKGYSLNEQVIRPAMVMVAQ

Sequences:

>Translated_194_residues
MNNEEKKVNEEIVVEDQIEAIGTEADVEWNESMEESQDAKIAELEAALLASQAQLKEQQDAVLRAKAEEQNVRRRAEGDI
DKARKYALKKFAGELLPVIDNLERALESGDKENEAAKVLLEGVELTLQTFISTIEKFGLTVINPVGETFNPEHHQAIGMQ
ASPDHESNTVMVVMQKGYSLNEQVIRPAMVMVAQ
>Mature_194_residues
MNNEEKKVNEEIVVEDQIEAIGTEADVEWNESMEESQDAKIAELEAALLASQAQLKEQQDAVLRAKAEEQNVRRRAEGDI
DKARKYALKKFAGELLPVIDNLERALESGDKENEAAKVLLEGVELTLQTFISTIEKFGLTVINPVGETFNPEHHQAIGMQ
ASPDHESNTVMVVMQKGYSLNEQVIRPAMVMVAQ

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=146, Percent_Identity=34.9315068493151, Blast_Score=83, Evalue=1e-16,
Organism=Homo sapiens, GI40255109, Length=148, Percent_Identity=33.7837837837838, Blast_Score=67, Evalue=8e-12,
Organism=Escherichia coli, GI1788967, Length=192, Percent_Identity=46.3541666666667, Blast_Score=152, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17552458, Length=151, Percent_Identity=37.0860927152318, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324806, Length=176, Percent_Identity=36.3636363636364, Blast_Score=108, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24653432, Length=158, Percent_Identity=31.0126582278481, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_ALISL (B6EKA3)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263823.1
- GeneID:   6987772
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2473
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- ProtClustDB:   CLSK2396207
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21618; Mature: 21618

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNEEKKVNEEIVVEDQIEAIGTEADVEWNESMEESQDAKIAELEAALLASQAQLKEQQD
CCCCHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AVLRAKAEEQNVRRRAEGDIDKARKYALKKFAGELLPVIDNLERALESGDKENEAAKVLL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EGVELTLQTFISTIEKFGLTVINPVGETFNPEHHQAIGMQASPDHESNTVMVVMQKGYSL
HHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCC
NEQVIRPAMVMVAQ
CHHHHHHHHHHHCC
>Mature Secondary Structure
MNNEEKKVNEEIVVEDQIEAIGTEADVEWNESMEESQDAKIAELEAALLASQAQLKEQQD
CCCCHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AVLRAKAEEQNVRRRAEGDIDKARKYALKKFAGELLPVIDNLERALESGDKENEAAKVLL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EGVELTLQTFISTIEKFGLTVINPVGETFNPEHHQAIGMQASPDHESNTVMVVMQKGYSL
HHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCC
NEQVIRPAMVMVAQ
CHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA