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The map label for this gene is tgt

Identifier: 209695859

GI number: 209695859

Start: 2606984

End: 2608111

Strand: Reverse

Name: tgt

Synonym: VSAL_I2435

Alternate gene names: 209695859

Gene position: 2608111-2606984 (Counterclockwise)

Preceding gene: 209695860

Following gene: 209695858

Centisome position: 78.44

GC content: 41.84

Gene sequence:

>1128_bases
GTGAAATACGAACTGATCAAAAAACAAGGCCGCGCACGTCGTGGCCAACTGCAATTCGACCGTGGTACGGTTGAAACTCC
TGCGTTTATGCCTGTTGGTACTTACGGTACTGTTAAAGGTATGACTCCTGAAGAAGTAAAAGATACAGGTGCAGAAATCT
TACTAGGTAACACATTCCATTTATGGTTGCGTCCTGGTCAAGAAATCATGAAATTGCATGGTGACTTGCATGATTTCATG
AATTGGAAAGGTCCTATTTTAACCGATTCAGGTGGTTTCCAAGTATTTAGCCTTGGTAAAACACGTAAAATCACAGAAGA
GGGCGTGCATTTCCGTAGCCCTGTAAACGGTGATAAGATCTTCATGGATGCAGAGAAATCAATGCAAATCCAATATGATC
TTGGTTCTGACGTTGTCATGATTTTTGATGAATGTACGCCATACCCAGCAACGCACGATGAAGCTCGTATTTCAATGGAA
CGTTCAATTCGTTGGGCTGATCGCAGCCGTAATGAGTTTGATCGCCAAGAAAACCCGAATGCATTATTTGGTATCGTTCA
AGGTGGTGTATACGAAGATTTACGTGATGTTTCTGTTGAAGCACTAACGAAAATTGGTTTTGATGGTTATGCTGTTGGTG
GTCTAGCAGTAGGCGAGCCAAAAGAAGACATGCACCGCATTTTAGAGCATACGTGCCCACTACTACCTGAAGATAAGCCA
CGATACCTAATGGGGGTTGGTAAACCAGAAGATTTGGTTGAAGGCGTTCGTCGCGGTATTGATATGTTTGACTGTGTTAT
GCCAACGCGTAATGCTCGTAATGGTCACTTATTTGTGACTGGCGGTATCGTTAAGATTCGTAATGCGAAACATAAAGTAG
ATACAACACCACTGGATCCTGAATGTGATTGTTATACTTGTCAGAACTACAGTAAATCTTATTTACATCATTTAGATCGC
TGTAATGAAATTTTAGGTGCACGTTTAAATACGATTCATAACTTACGTTATTACCAACGTATTATGGCGAGCATCCGTAA
GGCCTTGGAAGAAGATCGTTTCGAGCAGTTTGTAGAAGAGTTCTATGCACGCCGTGACCGTGAAGTTCCGCCATTAAAAG
ACCTTTAA

Upstream 100 bases:

>100_bases
TTTCAGTGAAAAGAGTAGCTAGAGGTGCATTTATTAAATGTCTTAATCAATGCAAAATAAACAAGTCGTCAGATTGTTTC
TCTGGCTTATTGGAGGTTTC

Downstream 100 bases:

>100_bases
AAGCAGAGTAATCACCCATATATCCATAGGGATATGTGTATGGTCACATTTTAGAAACCGGAAATTAGAAACTAGATACG
AGGACGTTATTAAATGAATT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKYELIKKQGRARRGQLQFDRGTVETPAFMPVGTYGTVKGMTPEEVKDTGAEILLGNTFHLWLRPGQEIMKLHGDLHDFM
NWKGPILTDSGGFQVFSLGKTRKITEEGVHFRSPVNGDKIFMDAEKSMQIQYDLGSDVVMIFDECTPYPATHDEARISME
RSIRWADRSRNEFDRQENPNALFGIVQGGVYEDLRDVSVEALTKIGFDGYAVGGLAVGEPKEDMHRILEHTCPLLPEDKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGIVKIRNAKHKVDTTPLDPECDCYTCQNYSKSYLHHLDR
CNEILGARLNTIHNLRYYQRIMASIRKALEEDRFEQFVEEFYARRDREVPPLKDL

Sequences:

>Translated_375_residues
MKYELIKKQGRARRGQLQFDRGTVETPAFMPVGTYGTVKGMTPEEVKDTGAEILLGNTFHLWLRPGQEIMKLHGDLHDFM
NWKGPILTDSGGFQVFSLGKTRKITEEGVHFRSPVNGDKIFMDAEKSMQIQYDLGSDVVMIFDECTPYPATHDEARISME
RSIRWADRSRNEFDRQENPNALFGIVQGGVYEDLRDVSVEALTKIGFDGYAVGGLAVGEPKEDMHRILEHTCPLLPEDKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGIVKIRNAKHKVDTTPLDPECDCYTCQNYSKSYLHHLDR
CNEILGARLNTIHNLRYYQRIMASIRKALEEDRFEQFVEEFYARRDREVPPLKDL
>Mature_375_residues
MKYELIKKQGRARRGQLQFDRGTVETPAFMPVGTYGTVKGMTPEEVKDTGAEILLGNTFHLWLRPGQEIMKLHGDLHDFM
NWKGPILTDSGGFQVFSLGKTRKITEEGVHFRSPVNGDKIFMDAEKSMQIQYDLGSDVVMIFDECTPYPATHDEARISME
RSIRWADRSRNEFDRQENPNALFGIVQGGVYEDLRDVSVEALTKIGFDGYAVGGLAVGEPKEDMHRILEHTCPLLPEDKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGIVKIRNAKHKVDTTPLDPECDCYTCQNYSKSYLHHLDR
CNEILGARLNTIHNLRYYQRIMASIRKALEEDRFEQFVEEFYARRDREVPPLKDL

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=354, Percent_Identity=45.7627118644068, Blast_Score=322, Evalue=3e-88,
Organism=Escherichia coli, GI1786607, Length=373, Percent_Identity=80.6970509383378, Blast_Score=642, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544680, Length=362, Percent_Identity=42.5414364640884, Blast_Score=303, Evalue=7e-83,
Organism=Caenorhabditis elegans, GI25146502, Length=213, Percent_Identity=30.0469483568075, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI19920510, Length=361, Percent_Identity=45.983379501385, Blast_Score=323, Evalue=8e-89,
Organism=Drosophila melanogaster, GI21355311, Length=163, Percent_Identity=33.1288343558282, Blast_Score=91, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_ALISL (B6EK65)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263789.1
- GeneID:   6987750
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2435
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 42940; Mature: 42940

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: ACT_SITE 89-89 BINDING 90-90

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYELIKKQGRARRGQLQFDRGTVETPAFMPVGTYGTVKGMTPEEVKDTGAEILLGNTFH
CCHHHHHHHCCCCCCCEEECCCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGQEIMKLHGDLHDFMNWKGPILTDSGGFQVFSLGKTRKITEEGVHFRSPVNGDKI
EEECCCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCCHHHHHCCCEECCCCCCCEE
FMDAEKSMQIQYDLGSDVVMIFDECTPYPATHDEARISMERSIRWADRSRNEFDRQENPN
EEECCCCEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCC
ALFGIVQGGVYEDLRDVSVEALTKIGFDGYAVGGLAVGEPKEDMHRILEHTCPLLPEDKP
EEEEEECCCHHHHHHHHHHHHHHHCCCCCEEECCEECCCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGIVKIRNAKHKVDTTPLDP
CEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCCCC
ECDCYTCQNYSKSYLHHLDRCNEILGARLNTIHNLRYYQRIMASIRKALEEDRFEQFVEE
CCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYARRDREVPPLKDL
HHHHCCCCCCCCCCC
>Mature Secondary Structure
MKYELIKKQGRARRGQLQFDRGTVETPAFMPVGTYGTVKGMTPEEVKDTGAEILLGNTFH
CCHHHHHHHCCCCCCCEEECCCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGQEIMKLHGDLHDFMNWKGPILTDSGGFQVFSLGKTRKITEEGVHFRSPVNGDKI
EEECCCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCCHHHHHCCCEECCCCCCCEE
FMDAEKSMQIQYDLGSDVVMIFDECTPYPATHDEARISMERSIRWADRSRNEFDRQENPN
EEECCCCEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCC
ALFGIVQGGVYEDLRDVSVEALTKIGFDGYAVGGLAVGEPKEDMHRILEHTCPLLPEDKP
EEEEEECCCHHHHHHHHHHHHHHHCCCCCEEECCEECCCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGIVKIRNAKHKVDTTPLDP
CEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCCCC
ECDCYTCQNYSKSYLHHLDRCNEILGARLNTIHNLRYYQRIMASIRKALEEDRFEQFVEE
CCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYARRDREVPPLKDL
HHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA