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The map label for this gene is queA

Identifier: 209695860

GI number: 209695860

Start: 2608304

End: 2609356

Strand: Reverse

Name: queA

Synonym: VSAL_I2436

Alternate gene names: 209695860

Gene position: 2609356-2608304 (Counterclockwise)

Preceding gene: 209695862

Following gene: 209695859

Centisome position: 78.47

GC content: 40.65

Gene sequence:

>1053_bases
ATGCAAGTATCCGATTTCCATTTTGAATTACCTGATGAACTTATTGCGCGTTATCCTCAACCAGAGCGTACCGCAAGTCG
CCTATTACATTTAAACGGTAATAGTGGTGAATTGCATGATGGTCAGTTTACTGATGTGCTTGATTTAGTACAGCATGGTG
ATCTTGTTGTATTTAATAATACGCGTGTTATTCCTGCTCGTATGTTTGGTGTTAAAGCCTCTGGCGGTAAACTGGAAGTG
TTAGTTGAGCGCGTTCTTGATGATCATTCTGTGTTGGCTCATGTTCGTTGTTCAAAATCACCAAAACCAGGAAATCAACT
GTGGTTAGGTGAGAACCAAGAATACGAAGCAGAGATGGTTGCACGTCACGATACATTATTTGAAATTCGTTTTACTTCAG
AAAAGAAAGTATTGGATATTTTGGATGAAATTGGCCATATGCCATTACCACCTTATATTGATCGTCCTGATGAAGATGCG
GATAAAGAACGTTATCAAACGGTTTACAATGAAAAACCGGGAGCGGTCGCTGCTCCAACTGCTGGTTTACACTTTGATAC
AGACATTTTAGAAAAGATGAAAGCAAAAGGGGTCGAATTTGCTTACGTCACCCTTCATGTTGGCGCTGGAACCTTTCAAC
CAGTACGGGTTGATAATATTTTAGATCACCATATGCATTCTGAATATGCCGAAGTATCTCAAGAAGTCGTTGATGCAATT
AAAGCCACGAAGGCTCGTGGAGGTCGCGTTATTTCTGTTGGGACGACATCGGTTCGTTCTTTAGAAAGTGCAGCACAACA
CGCTTTAAAACAAGGGACGGAGTTAGCGCCATTTTTTGATGATACTGAAATATTTATTTACCCAGGGTATGAATTTCAAG
TCGTTGACGCATTGGTGACAAACTTTCACCTGCCAGAGTCGACATTAATCATGTTAGTCAGTGCTTTTGCAGGATATGAC
AATACAATGAAAGCGTACGATCAAGCCGTAAATCATCAATACCGTTTCTTTAGTTACGGTGATGCGATGTTTATTACGAA
GAAGACGTCGTAG

Upstream 100 bases:

>100_bases
TATTTTAGGAAAAAATGAGAGGTATATACCTTCACCTCTTGCTATTGCTCTTCGCGTGAATATACTACTCTGTCTGCCAT
TTTTGATGAAGAAGTAAACC

Downstream 100 bases:

>100_bases
TAAAAAGTGAGTTAGTGCTCATTCTTAATTCAAACTTCACTCACGGAGCCAGTGAAATAAGGAAAACAGGACGTTTTCAG
CTAGACTACTCCTTTCAGTG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MQVSDFHFELPDELIARYPQPERTASRLLHLNGNSGELHDGQFTDVLDLVQHGDLVVFNNTRVIPARMFGVKASGGKLEV
LVERVLDDHSVLAHVRCSKSPKPGNQLWLGENQEYEAEMVARHDTLFEIRFTSEKKVLDILDEIGHMPLPPYIDRPDEDA
DKERYQTVYNEKPGAVAAPTAGLHFDTDILEKMKAKGVEFAYVTLHVGAGTFQPVRVDNILDHHMHSEYAEVSQEVVDAI
KATKARGGRVISVGTTSVRSLESAAQHALKQGTELAPFFDDTEIFIYPGYEFQVVDALVTNFHLPESTLIMLVSAFAGYD
NTMKAYDQAVNHQYRFFSYGDAMFITKKTS

Sequences:

>Translated_350_residues
MQVSDFHFELPDELIARYPQPERTASRLLHLNGNSGELHDGQFTDVLDLVQHGDLVVFNNTRVIPARMFGVKASGGKLEV
LVERVLDDHSVLAHVRCSKSPKPGNQLWLGENQEYEAEMVARHDTLFEIRFTSEKKVLDILDEIGHMPLPPYIDRPDEDA
DKERYQTVYNEKPGAVAAPTAGLHFDTDILEKMKAKGVEFAYVTLHVGAGTFQPVRVDNILDHHMHSEYAEVSQEVVDAI
KATKARGGRVISVGTTSVRSLESAAQHALKQGTELAPFFDDTEIFIYPGYEFQVVDALVTNFHLPESTLIMLVSAFAGYD
NTMKAYDQAVNHQYRFFSYGDAMFITKKTS
>Mature_350_residues
MQVSDFHFELPDELIARYPQPERTASRLLHLNGNSGELHDGQFTDVLDLVQHGDLVVFNNTRVIPARMFGVKASGGKLEV
LVERVLDDHSVLAHVRCSKSPKPGNQLWLGENQEYEAEMVARHDTLFEIRFTSEKKVLDILDEIGHMPLPPYIDRPDEDA
DKERYQTVYNEKPGAVAAPTAGLHFDTDILEKMKAKGVEFAYVTLHVGAGTFQPVRVDNILDHHMHSEYAEVSQEVVDAI
KATKARGGRVISVGTTSVRSLESAAQHALKQGTELAPFFDDTEIFIYPGYEFQVVDALVTNFHLPESTLIMLVSAFAGYD
NTMKAYDQAVNHQYRFFSYGDAMFITKKTS

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=72.2543352601156, Blast_Score=518, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_ALISL (B6EK66)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263790.1
- GeneID:   6989280
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2436
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39288; Mature: 39288

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSDFHFELPDELIARYPQPERTASRLLHLNGNSGELHDGQFTDVLDLVQHGDLVVFNN
CCCCCCCCCCCHHHHHCCCCCHHHHHHEEEEECCCCCCCCCCHHHHHHHHHCCCEEEECC
TRVIPARMFGVKASGGKLEVLVERVLDDHSVLAHVRCSKSPKPGNQLWLGENQEYEAEMV
CEEEEHHEEEEECCCCHHHHHHHHHHCCHHHEEEEEECCCCCCCCEEECCCCCCHHHHHH
ARHDTLFEIRFTSEKKVLDILDEIGHMPLPPYIDRPDEDADKERYQTVYNEKPGAVAAPT
HCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCC
AGLHFDTDILEKMKAKGVEFAYVTLHVGAGTFQPVRVDNILDHHMHSEYAEVSQEVVDAI
CCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
KATKARGGRVISVGTTSVRSLESAAQHALKQGTELAPFFDDTEIFIYPGYEFQVVDALVT
HHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEHHHHHHH
NFHLPESTLIMLVSAFAGYDNTMKAYDQAVNHQYRFFSYGDAMFITKKTS
HCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCEEEEEECCC
>Mature Secondary Structure
MQVSDFHFELPDELIARYPQPERTASRLLHLNGNSGELHDGQFTDVLDLVQHGDLVVFNN
CCCCCCCCCCCHHHHHCCCCCHHHHHHEEEEECCCCCCCCCCHHHHHHHHHCCCEEEECC
TRVIPARMFGVKASGGKLEVLVERVLDDHSVLAHVRCSKSPKPGNQLWLGENQEYEAEMV
CEEEEHHEEEEECCCCHHHHHHHHHHCCHHHEEEEEECCCCCCCCEEECCCCCCHHHHHH
ARHDTLFEIRFTSEKKVLDILDEIGHMPLPPYIDRPDEDADKERYQTVYNEKPGAVAAPT
HCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCC
AGLHFDTDILEKMKAKGVEFAYVTLHVGAGTFQPVRVDNILDHHMHSEYAEVSQEVVDAI
CCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
KATKARGGRVISVGTTSVRSLESAAQHALKQGTELAPFFDDTEIFIYPGYEFQVVDALVT
HHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEHHHHHHH
NFHLPESTLIMLVSAFAGYDNTMKAYDQAVNHQYRFFSYGDAMFITKKTS
HCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA