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The map label for this gene is ruvB

Identifier: 209695356

GI number: 209695356

Start: 2024185

End: 2025198

Strand: Direct

Name: ruvB

Synonym: VSAL_I1889

Alternate gene names: 209695356

Gene position: 2024185-2025198 (Clockwise)

Preceding gene: 209695355

Following gene: 209695357

Centisome position: 60.87

GC content: 41.22

Gene sequence:

>1014_bases
ATGATTGAAGCCGATCGCCTTATTGCTGCTGATAACCCTGTTTTTCGTGACGAAGAAGTCATCGATCGTGCGATACGTCC
AAAGAAACTGGAAGATTATCGCGGACAAGATCATGTTCGTAGCCAAATGGAAATTTTCATTAAGGCTGCGCAGATGAGAC
AAGAGCCACTTGATCATCTACTAATTTTTGGTCCTCCAGGATTAGGTAAGACAACGTTAGCTAATATTGTTGCCAATGAA
ATGGGAGTGAGTATCCGAACGACATCGGGTCCTGTTCTTGAGAAAGCGGGAGATTTAGCTGCGCTTCTTACTAATCTGGA
AGAAAACGATATTTTATTTATCGATGAAATCCATCGATTAAGTCCTGTAGTAGAAGAGATACTGTATCCTGCGATGGAAG
ATTACCAATTAGATATTATGATTGGTGAAGGTCCTGCAGCTCGTTCAATCAAAATTGATTTGCCCCCATTTACATTAATT
GGTGCAACCACCAGAGCCGGTTCTTTGACTTCTCCACTGCGTGACCGTTTTGGTATTGTTCAACGATTAGAATATTACAA
AGTAGACGATTTGCAATATATCGTTCAACGTAGTGCGGATTGTTTGAATTTGTCCATGGAATCAGAGGGCGCATTGGAAG
TGGCTCGACGTGCTCGTGGTACACCACGAATTGCCAACCGATTGTTACGTCGAGTTCGTGATTATGCAGATGTTATGAGT
GACAGTCATATCTCACCAGACATAGCAGATAAGGCATTAAACATGTTAGATGTTGATGTGTGTGGGTTTGATTACATGGA
TAGAAAGTTATTATTGGCAATCATGGAGAAATTCAATGGTGGTCCAGTGGGTCTAGATAACGTAGCCGCTGCAATTGGTG
AAGAAAAAGATACGATTGAAGATGTGATAGAACCTTATTTAATTCAACAAGGCTACCTTCAGAGAACACCAAGAGGGCGT
ATTGTGTCAGATCGAGCCTATCTTCATTTTGGTATTGATAGACCAGATAAATAG

Upstream 100 bases:

>100_bases
ACCTGATATGACCAGTGAATCTCTAATTCGTGAATCATTGAAATCAATGATCTAACTTTATTTATCGCCTCTTTACTTAC
AGAATTTTTTGAGACCTTTT

Downstream 100 bases:

>100_bases
GTAATGGTTAAATATACACATATTGTCATAATCTATTCATATTGACGTGAAAAAAATGAAAGCGAGTGATCAAAATCACT
CGTTTTTTCGTATAAGAAAT

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MIEADRLIAADNPVFRDEEVIDRAIRPKKLEDYRGQDHVRSQMEIFIKAAQMRQEPLDHLLIFGPPGLGKTTLANIVANE
MGVSIRTTSGPVLEKAGDLAALLTNLEENDILFIDEIHRLSPVVEEILYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLI
GATTRAGSLTSPLRDRFGIVQRLEYYKVDDLQYIVQRSADCLNLSMESEGALEVARRARGTPRIANRLLRRVRDYADVMS
DSHISPDIADKALNMLDVDVCGFDYMDRKLLLAIMEKFNGGPVGLDNVAAAIGEEKDTIEDVIEPYLIQQGYLQRTPRGR
IVSDRAYLHFGIDRPDK

Sequences:

>Translated_337_residues
MIEADRLIAADNPVFRDEEVIDRAIRPKKLEDYRGQDHVRSQMEIFIKAAQMRQEPLDHLLIFGPPGLGKTTLANIVANE
MGVSIRTTSGPVLEKAGDLAALLTNLEENDILFIDEIHRLSPVVEEILYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLI
GATTRAGSLTSPLRDRFGIVQRLEYYKVDDLQYIVQRSADCLNLSMESEGALEVARRARGTPRIANRLLRRVRDYADVMS
DSHISPDIADKALNMLDVDVCGFDYMDRKLLLAIMEKFNGGPVGLDNVAAAIGEEKDTIEDVIEPYLIQQGYLQRTPRGR
IVSDRAYLHFGIDRPDK
>Mature_337_residues
MIEADRLIAADNPVFRDEEVIDRAIRPKKLEDYRGQDHVRSQMEIFIKAAQMRQEPLDHLLIFGPPGLGKTTLANIVANE
MGVSIRTTSGPVLEKAGDLAALLTNLEENDILFIDEIHRLSPVVEEILYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLI
GATTRAGSLTSPLRDRFGIVQRLEYYKVDDLQYIVQRSADCLNLSMESEGALEVARRARGTPRIANRLLRRVRDYADVMS
DSHISPDIADKALNMLDVDVCGFDYMDRKLLLAIMEKFNGGPVGLDNVAAAIGEEKDTIEDVIEPYLIQQGYLQRTPRGR
IVSDRAYLHFGIDRPDK

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=336, Percent_Identity=79.1666666666667, Blast_Score=532, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_ALISL (B6EGJ4)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263285.1
- GeneID:   6989216
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I1889
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 37821; Mature: 37821

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEADRLIAADNPVFRDEEVIDRAIRPKKLEDYRGQDHVRSQMEIFIKAAQMRQEPLDHL
CCCCCCEEECCCCCCCCHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHEE
LIFGPPGLGKTTLANIVANEMGVSIRTTSGPVLEKAGDLAALLTNLEENDILFIDEIHRL
EEECCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHH
SPVVEEILYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGATTRAGSLTSPLRDRFGIV
HHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHHHHH
QRLEYYKVDDLQYIVQRSADCLNLSMESEGALEVARRARGTPRIANRLLRRVRDYADVMS
HHHHHHCHHHHHHHHHCCCHHEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
DSHISPDIADKALNMLDVDVCGFDYMDRKLLLAIMEKFNGGPVGLDNVAAAIGEEKDTIE
CCCCCCHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHH
DVIEPYLIQQGYLQRTPRGRIVSDRAYLHFGIDRPDK
HHHHHHHHHHHHHHCCCCCCEEECCEEEEECCCCCCC
>Mature Secondary Structure
MIEADRLIAADNPVFRDEEVIDRAIRPKKLEDYRGQDHVRSQMEIFIKAAQMRQEPLDHL
CCCCCCEEECCCCCCCCHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHEE
LIFGPPGLGKTTLANIVANEMGVSIRTTSGPVLEKAGDLAALLTNLEENDILFIDEIHRL
EEECCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHH
SPVVEEILYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGATTRAGSLTSPLRDRFGIV
HHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHHHHH
QRLEYYKVDDLQYIVQRSADCLNLSMESEGALEVARRARGTPRIANRLLRRVRDYADVMS
HHHHHHCHHHHHHHHHCCCHHEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
DSHISPDIADKALNMLDVDVCGFDYMDRKLLLAIMEKFNGGPVGLDNVAAAIGEEKDTIE
CCCCCCHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHH
DVIEPYLIQQGYLQRTPRGRIVSDRAYLHFGIDRPDK
HHHHHHHHHHHHHHCCCCCCEEECCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA