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The map label for this gene is ruvC

Identifier: 209695354

GI number: 209695354

Start: 2022901

End: 2023422

Strand: Direct

Name: ruvC

Synonym: VSAL_I1887

Alternate gene names: 209695354

Gene position: 2022901-2023422 (Clockwise)

Preceding gene: 209695353

Following gene: 209695355

Centisome position: 60.84

GC content: 43.87

Gene sequence:

>522_bases
ATGTCTATAATTTTAGGAATTGATCCCGGCTCTCGTATTACCGGTTATGGCGTTATTCGTCAAAACGGCCGTCATTTGCA
ATATTTAGGGAGTGGTTGTATTCGGATGTCAGAAAAAGAACTTCCCGGTCGCTTAAAGCAGATCTATGCTGGCGTTTCTG
AAATTATTACGCAGTTTCAACCAGATGTATTCGCGATCGAGCAAGTATTTATGTCAAAAAATGCGGATTCAGCACTTAAA
TTAGGACAAGCAAGAGGAAGTGCGATTGTTGCTGCCGTTAATGCAGACCTACCTGTATATGAATATGCTGCTCGTTTAAT
TAAACAAGCCGTAACGGGAACGGGTGCAGCCGATAAAGCTCAAGTTCAGCATATGGTTATGACGATGCTTAAATTACCAG
CAAGACCACAAGCGGATGCCGCTGATGGTTTAGGGGTTGCAATTTGTCATGCGAATACGAATAAAACGTTAATCGCCTTA
GCGGGGAAAGCGACAGGAGCAAGGCGAGGACGATATCGCTAG

Upstream 100 bases:

>100_bases
AATTAATTAAAGTTTTCTTTTTATTAAAAAATGCCCGCCTTATGCGGGCATTTTTGTATATACTATAACTGTATAAATAA
TCAGTGGTTTAAATAATGCT

Downstream 100 bases:

>100_bases
ATAAAAGAGGGAATAAATTCTCATTTCTTTTACTGGATATCTATCCAGTTCTTTATTACTATCCTAACCAGTTAATTATT
TTAAAGAGTATCTATTGTGA

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MSIILGIDPGSRITGYGVIRQNGRHLQYLGSGCIRMSEKELPGRLKQIYAGVSEIITQFQPDVFAIEQVFMSKNADSALK
LGQARGSAIVAAVNADLPVYEYAARLIKQAVTGTGAADKAQVQHMVMTMLKLPARPQADAADGLGVAICHANTNKTLIAL
AGKATGARRGRYR

Sequences:

>Translated_173_residues
MSIILGIDPGSRITGYGVIRQNGRHLQYLGSGCIRMSEKELPGRLKQIYAGVSEIITQFQPDVFAIEQVFMSKNADSALK
LGQARGSAIVAAVNADLPVYEYAARLIKQAVTGTGAADKAQVQHMVMTMLKLPARPQADAADGLGVAICHANTNKTLIAL
AGKATGARRGRYR
>Mature_172_residues
SIILGIDPGSRITGYGVIRQNGRHLQYLGSGCIRMSEKELPGRLKQIYAGVSEIITQFQPDVFAIEQVFMSKNADSALKL
GQARGSAIVAAVNADLPVYEYAARLIKQAVTGTGAADKAQVQHMVMTMLKLPARPQADAADGLGVAICHANTNKTLIALA
GKATGARRGRYR

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=173, Percent_Identity=69.364161849711, Blast_Score=228, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_ALISL (B6EGJ2)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263283.1
- GeneID:   6988274
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I1887
- HOGENOM:   HBG437552
- OMA:   PMQIKLA
- ProtClustDB:   PRK00039
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 18454; Mature: 18323

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIILGIDPGSRITGYGVIRQNGRHLQYLGSGCIRMSEKELPGRLKQIYAGVSEIITQFQ
CEEEEECCCCCCEEEEEEEECCCCEEEECCCCHHCCCCHHCCHHHHHHHHHHHHHHHHHC
PDVFAIEQVFMSKNADSALKLGQARGSAIVAAVNADLPVYEYAARLIKQAVTGTGAADKA
CCHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHH
QVQHMVMTMLKLPARPQADAADGLGVAICHANTNKTLIALAGKATGARRGRYR
HHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SIILGIDPGSRITGYGVIRQNGRHLQYLGSGCIRMSEKELPGRLKQIYAGVSEIITQFQ
EEEEECCCCCCEEEEEEEECCCCEEEECCCCHHCCCCHHCCHHHHHHHHHHHHHHHHHC
PDVFAIEQVFMSKNADSALKLGQARGSAIVAAVNADLPVYEYAARLIKQAVTGTGAADKA
CCHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHH
QVQHMVMTMLKLPARPQADAADGLGVAICHANTNKTLIALAGKATGARRGRYR
HHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA