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The map label for this gene is aspS [H]

Identifier: 209695353

GI number: 209695353

Start: 2021027

End: 2022802

Strand: Direct

Name: aspS [H]

Synonym: VSAL_I1886

Alternate gene names: 209695353

Gene position: 2021027-2022802 (Clockwise)

Preceding gene: 209695347

Following gene: 209695354

Centisome position: 60.78

GC content: 42.85

Gene sequence:

>1776_bases
ATGCGCAGCCATTACTGTGGTAACTTGAACAAGTCCCTTGCAGGACAAACTGTAGAACTTTGCGGCTGGGTAAACCGTCG
CCGTGATTTAGGCGGTCTTATCTTCATTGATATGCGAGATCGTGAAGGTGTCGTTCAGGTAGTTGTTGACCCAGATATGA
AAGATATCTTTTCAACTGCTAACCAACTGCGCAATGAATTCTGTATTAAATTTACTGGCGAAGTACGTGTTCGTCCTGAC
AGCCAAGTAAATAAAGATATGTCTACGGGTGAAGTTGAACTTTACGCGACAGGTCTTGAGATCATCAACCGTTCTGAAGC
ACTTCCATTGGATTTCAATCAAACGAACTCAGAAGAGCAACGCCTGAAGTACCGTTATATTGATTTACGTCGTCCAGAAA
TGAGTGACCGTATCAAGTTACGTGCTCGTGCATCTAGCTTTGTTCGTCGTTTCTTAGATGAGAACCTATTCTTAGATATC
GAAACACCAGTACTAACAAAAGCAACGCCAGAAGGCGCTCGTGATTACCTAGTACCAAGCCGTGTTCATAAAGGCAGCTT
CTACGCACTTCCACAATCACCACAGTTGTTTAAGCAACTTCTGATGATGTCTGGTTTTGACCGTTACTACCAAATCGTTA
AATGTTTCCGTGATGAAGATTTACGCGCTGACCGCCAACCTGAATTTACTCAAATAGATATCGAAACCTCTTTCCTTTCG
TCTAACCAAGTTCGTGATATTACTGAACGTTTGGTTCATGACATGTGGAAAGAACTTCTTGATGTTGAGCTTGGTCAATT
TCCAATAATGAAATTCTCTGAAGCGATTCGTCGTTTTGGTTCTGATAAGCCAGATCTACGTAACCCACTAGAATTAGTGG
ACATTGCAGATCTTCTTAAAGACGTAGAATTCCAAGTATTCGCTGGTCCTGCAAATGACGAAAAAGGCCGTGTAGCGGTT
ATTCGTGTTCCTGGTGGCGCATCGCTTTCTCGTAAGCAAATTGATGAATACGGCAAATTTGTTGGTATTTACGGCGCGAA
AGGCCTTGCTTGGATGAAAGTAAACGATCGTGCTGCTGGTCTTGAAGGAGTTCAATCTCCTGTTGCTAAATTCCTAAACG
CAGACGTTGTAAATGGTATTTTAGAACGCACAGAAGCTGAAAGTGGCGATATCATCCTATTTGGTGCAGATAAAGCAAAC
ATTGTTTCTGAAGCCATGGGTGCATTACGTATCAAGCTTGGTGATGACTTAGAACTAACAGACAAAAAAGCATGGGCACC
TCTTTGGGTTATTGATTTCCCAATGTTTGAAGAAGATGGCGAAGGTAACTTGCATGCAATGCACCACCCATTTACTTCTC
CATTAGGCATGACAGCGGAAGAGCTTAAAGTAAACCCTGCTGCTGCAAACTCTGATGCTTACGATATGGTTATCAACGGC
TATGAAGTTGGTGGTGGCTCTGTACGTATTCACAATGCTGAAATGCAAACTGCAGTATTCGGTATCCTAGGTATTGAAGC
GAAAGAGCAACAAGAGAAATTTGGTTTCTTACTTGAAGCGCTTAAATACGGTACGCCGCCACACGCAGGTCTTGCTTTCG
GTCTTGACCGTCTTGCAATGCTACTTTGCGGTACTGAAAACATTCGTGACGTTATTGCATTCCCTAAAACAACGGCTGCA
GCATGTTTACTTACGAATGCTCCAAGCCCTGCGAATCCAGATTCTCTTGCAGAATTAGCGATTGCAGTAAAATTTGCTGA
GAAAAAAGACGCTTAA

Upstream 100 bases:

>100_bases
TTCATCATTTAAACAAACGTAGAATCAGTTTATGAAAAATGCAGTGAGACTATTTCACTGCATTTATTATTGATATGTAT
ATTTAAATTGGGAAATCATT

Downstream 100 bases:

>100_bases
TTAATTAAAGTTTTCTTTTTATTAAAAAATGCCCGCCTTATGCGGGCATTTTTGTATATACTATAACTGTATAAATAATC
AGTGGTTTAAATAATGCTAT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MRSHYCGNLNKSLAGQTVELCGWVNRRRDLGGLIFIDMRDREGVVQVVVDPDMKDIFSTANQLRNEFCIKFTGEVRVRPD
SQVNKDMSTGEVELYATGLEIINRSEALPLDFNQTNSEEQRLKYRYIDLRRPEMSDRIKLRARASSFVRRFLDENLFLDI
ETPVLTKATPEGARDYLVPSRVHKGSFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSFLS
SNQVRDITERLVHDMWKELLDVELGQFPIMKFSEAIRRFGSDKPDLRNPLELVDIADLLKDVEFQVFAGPANDEKGRVAV
IRVPGGASLSRKQIDEYGKFVGIYGAKGLAWMKVNDRAAGLEGVQSPVAKFLNADVVNGILERTEAESGDIILFGADKAN
IVSEAMGALRIKLGDDLELTDKKAWAPLWVIDFPMFEEDGEGNLHAMHHPFTSPLGMTAEELKVNPAAANSDAYDMVING
YEVGGGSVRIHNAEMQTAVFGILGIEAKEQQEKFGFLLEALKYGTPPHAGLAFGLDRLAMLLCGTENIRDVIAFPKTTAA
ACLLTNAPSPANPDSLAELAIAVKFAEKKDA

Sequences:

>Translated_591_residues
MRSHYCGNLNKSLAGQTVELCGWVNRRRDLGGLIFIDMRDREGVVQVVVDPDMKDIFSTANQLRNEFCIKFTGEVRVRPD
SQVNKDMSTGEVELYATGLEIINRSEALPLDFNQTNSEEQRLKYRYIDLRRPEMSDRIKLRARASSFVRRFLDENLFLDI
ETPVLTKATPEGARDYLVPSRVHKGSFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSFLS
SNQVRDITERLVHDMWKELLDVELGQFPIMKFSEAIRRFGSDKPDLRNPLELVDIADLLKDVEFQVFAGPANDEKGRVAV
IRVPGGASLSRKQIDEYGKFVGIYGAKGLAWMKVNDRAAGLEGVQSPVAKFLNADVVNGILERTEAESGDIILFGADKAN
IVSEAMGALRIKLGDDLELTDKKAWAPLWVIDFPMFEEDGEGNLHAMHHPFTSPLGMTAEELKVNPAAANSDAYDMVING
YEVGGGSVRIHNAEMQTAVFGILGIEAKEQQEKFGFLLEALKYGTPPHAGLAFGLDRLAMLLCGTENIRDVIAFPKTTAA
ACLLTNAPSPANPDSLAELAIAVKFAEKKDA
>Mature_591_residues
MRSHYCGNLNKSLAGQTVELCGWVNRRRDLGGLIFIDMRDREGVVQVVVDPDMKDIFSTANQLRNEFCIKFTGEVRVRPD
SQVNKDMSTGEVELYATGLEIINRSEALPLDFNQTNSEEQRLKYRYIDLRRPEMSDRIKLRARASSFVRRFLDENLFLDI
ETPVLTKATPEGARDYLVPSRVHKGSFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSFLS
SNQVRDITERLVHDMWKELLDVELGQFPIMKFSEAIRRFGSDKPDLRNPLELVDIADLLKDVEFQVFAGPANDEKGRVAV
IRVPGGASLSRKQIDEYGKFVGIYGAKGLAWMKVNDRAAGLEGVQSPVAKFLNADVVNGILERTEAESGDIILFGADKAN
IVSEAMGALRIKLGDDLELTDKKAWAPLWVIDFPMFEEDGEGNLHAMHHPFTSPLGMTAEELKVNPAAANSDAYDMVING
YEVGGGSVRIHNAEMQTAVFGILGIEAKEQQEKFGFLLEALKYGTPPHAGLAFGLDRLAMLLCGTENIRDVIAFPKTTAA
ACLLTNAPSPANPDSLAELAIAVKFAEKKDA

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=599, Percent_Identity=38.89816360601, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI5031815, Length=257, Percent_Identity=26.4591439688716, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI194272210, Length=257, Percent_Identity=26.4591439688716, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI45439306, Length=254, Percent_Identity=27.1653543307087, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788173, Length=583, Percent_Identity=73.2418524871355, Blast_Score=910, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=307, Percent_Identity=25.0814332247557, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1790571, Length=288, Percent_Identity=24.6527777777778, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI32566633, Length=609, Percent_Identity=33.8259441707718, Blast_Score=311, Evalue=7e-85,
Organism=Caenorhabditis elegans, GI71994340, Length=248, Percent_Identity=23.7903225806452, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=248, Percent_Identity=23.7903225806452, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=248, Percent_Identity=23.7903225806452, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17551876, Length=261, Percent_Identity=26.8199233716475, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=637, Percent_Identity=33.7519623233909, Blast_Score=279, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6320242, Length=260, Percent_Identity=25, Blast_Score=77, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6323011, Length=279, Percent_Identity=27.2401433691756, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321807, Length=250, Percent_Identity=27.2, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24584738, Length=602, Percent_Identity=35.3820598006645, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24640849, Length=259, Percent_Identity=28.1853281853282, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24640851, Length=259, Percent_Identity=28.1853281853282, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17136276, Length=252, Percent_Identity=27.3809523809524, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66030; Mature: 66030

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSHYCGNLNKSLAGQTVELCGWVNRRRDLGGLIFIDMRDREGVVQVVVDPDMKDIFSTA
CCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCHHHHHHHH
NQLRNEFCIKFTGEVRVRPDSQVNKDMSTGEVELYATGLEIINRSEALPLDFNQTNSEEQ
HHHHHCEEEEEECCEEECCCCHHCCCCCCCEEEEEEECHHHHCCCCCCCCCCCCCCCHHH
RLKYRYIDLRRPEMSDRIKLRARASSFVRRFLDENLFLDIETPVLTKATPEGARDYLVPS
HHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCCCCCCCC
RVHKGSFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSFLS
CCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHCC
SNQVRDITERLVHDMWKELLDVELGQFPIMKFSEAIRRFGSDKPDLRNPLELVDIADLLK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DVEFQVFAGPANDEKGRVAVIRVPGGASLSRKQIDEYGKFVGIYGAKGLAWMKVNDRAAG
CCCEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHCEEEEECCCCEEEEEECCCCCC
LEGVQSPVAKFLNADVVNGILERTEAESGDIILFGADKANIVSEAMGALRIKLGDDLELT
HHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHEEEEEECCCCEEC
DKKAWAPLWVIDFPMFEEDGEGNLHAMHHPFTSPLGMTAEELKVNPAAANSDAYDMVING
CCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCCCCCHHHHCCCCCCCCCCCEEEEEEE
YEVGGGSVRIHNAEMQTAVFGILGIEAKEQQEKFGFLLEALKYGTPPHAGLAFGLDRLAM
EEECCCEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LLCGTENIRDVIAFPKTTAAACLLTNAPSPANPDSLAELAIAVKFAEKKDA
HHHCCCCHHHHHCCCCCHHEEEEEECCCCCCCHHHHHHHHHHEEEHHCCCC
>Mature Secondary Structure
MRSHYCGNLNKSLAGQTVELCGWVNRRRDLGGLIFIDMRDREGVVQVVVDPDMKDIFSTA
CCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCHHHHHHHH
NQLRNEFCIKFTGEVRVRPDSQVNKDMSTGEVELYATGLEIINRSEALPLDFNQTNSEEQ
HHHHHCEEEEEECCEEECCCCHHCCCCCCCEEEEEEECHHHHCCCCCCCCCCCCCCCHHH
RLKYRYIDLRRPEMSDRIKLRARASSFVRRFLDENLFLDIETPVLTKATPEGARDYLVPS
HHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCCCCCCCC
RVHKGSFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSFLS
CCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHCC
SNQVRDITERLVHDMWKELLDVELGQFPIMKFSEAIRRFGSDKPDLRNPLELVDIADLLK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DVEFQVFAGPANDEKGRVAVIRVPGGASLSRKQIDEYGKFVGIYGAKGLAWMKVNDRAAG
CCCEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHCEEEEECCCCEEEEEECCCCCC
LEGVQSPVAKFLNADVVNGILERTEAESGDIILFGADKANIVSEAMGALRIKLGDDLELT
HHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHEEEEEECCCCEEC
DKKAWAPLWVIDFPMFEEDGEGNLHAMHHPFTSPLGMTAEELKVNPAAANSDAYDMVING
CCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCCCCCHHHHCCCCCCCCCCCEEEEEEE
YEVGGGSVRIHNAEMQTAVFGILGIEAKEQQEKFGFLLEALKYGTPPHAGLAFGLDRLAM
EEECCCEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LLCGTENIRDVIAFPKTTAAACLLTNAPSPANPDSLAELAIAVKFAEKKDA
HHHCCCCHHHHHCCCCCHHEEEEEECCCCCCCHHHHHHHHHHEEEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA