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The map label for this gene is ipk

Identifier: 209694369

GI number: 209694369

Start: 873525

End: 874391

Strand: Reverse

Name: ipk

Synonym: VSAL_I0787

Alternate gene names: 209694369

Gene position: 874391-873525 (Counterclockwise)

Preceding gene: 209694370

Following gene: 209694368

Centisome position: 26.3

GC content: 39.91

Gene sequence:

>867_bases
ATGATAACGACAGCAACTCGCTGGCCTTCTCCAGCTAAACTCAATTTATTCCTGTATATAAATGGGCAGCATGATAATGG
TTATCATGAATTACAGACCCTTTTTCAATTTATCGATCTTTGTGATCACCTAACGATTACCCCTAACGATTCAGGAGAGA
TCACGCTTTCTCCTCCGATTCCCGGTGTAGAGAAAAAAGATAATTTAATCTGGAAAGCAGCAACGCAGCTACAAGAACAC
ACTCTGTGTCCTCTTGGTGCTCATATTGAACTAGAAAAAATATTACCCATTGGCGGTGGCATTGGCGGTGGTTCATCGAA
TGCAGCGACAACGTTAGTTGCACTTAATTTCTTATGGGATCTAAATCTCACGAATAAAGAATTGGCCGATTTAGGGGTCA
CTCTAGGCGCTGACGTTCCTATTTTTGTTCATGGTTTTTCCGCCTTTGCAGAGGGTATTGGTGAAAAATTACAACCGGCC
AACCCTAAAGAGTTATGGTACGTATTAATAAAACCAGAAGTGAGTATCGCAACTGTTGATGTATTCTCTCACCCAGAATT
GATTAGAAATACAGCAAAACAGCCACTTAACGTACTTTTAAGTGCCTCTTACGAAAACGATTGCGAAAAAATTGTCAGAA
GAGTGTATCCAGAGGTTGATTACCAACTTTCATGGCTGTTAGAATATGCACCATCAAGATTGACTGGAACAGGCGCTTGT
GTTTTCGCTGAGTTTATTAACGAAAAAGAAGCACAACACGTTTTTTCATTAATTCCTGATAACGCAACTGGATTTATCAC
CCGAGGTAGAAATACTTCACCACTCAACCAAGCGTTGGAAGAGTATAAATCATTATGCAACATATAA

Upstream 100 bases:

>100_bases
GCTATATCGAAACCAATGGGTTACTGCTGCCGAATTCAATGAAATTGACACAAAAAGACACCACAATAAAAATCATTGCT
TCAAATTGGATTATATCTCA

Downstream 100 bases:

>100_bases
AATTCTCACAATTTGGACGCAACCCTGAGGTTTCCACCGTGCCTGACATGAAGCTATTTACTGGTAATGCAACTCCTGAA
CTTGCCCAACGTATCGCCGA

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MITTATRWPSPAKLNLFLYINGQHDNGYHELQTLFQFIDLCDHLTITPNDSGEITLSPPIPGVEKKDNLIWKAATQLQEH
TLCPLGAHIELEKILPIGGGIGGGSSNAATTLVALNFLWDLNLTNKELADLGVTLGADVPIFVHGFSAFAEGIGEKLQPA
NPKELWYVLIKPEVSIATVDVFSHPELIRNTAKQPLNVLLSASYENDCEKIVRRVYPEVDYQLSWLLEYAPSRLTGTGAC
VFAEFINEKEAQHVFSLIPDNATGFITRGRNTSPLNQALEEYKSLCNI

Sequences:

>Translated_288_residues
MITTATRWPSPAKLNLFLYINGQHDNGYHELQTLFQFIDLCDHLTITPNDSGEITLSPPIPGVEKKDNLIWKAATQLQEH
TLCPLGAHIELEKILPIGGGIGGGSSNAATTLVALNFLWDLNLTNKELADLGVTLGADVPIFVHGFSAFAEGIGEKLQPA
NPKELWYVLIKPEVSIATVDVFSHPELIRNTAKQPLNVLLSASYENDCEKIVRRVYPEVDYQLSWLLEYAPSRLTGTGAC
VFAEFINEKEAQHVFSLIPDNATGFITRGRNTSPLNQALEEYKSLCNI
>Mature_288_residues
MITTATRWPSPAKLNLFLYINGQHDNGYHELQTLFQFIDLCDHLTITPNDSGEITLSPPIPGVEKKDNLIWKAATQLQEH
TLCPLGAHIELEKILPIGGGIGGGSSNAATTLVALNFLWDLNLTNKELADLGVTLGADVPIFVHGFSAFAEGIGEKLQPA
NPKELWYVLIKPEVSIATVDVFSHPELIRNTAKQPLNVLLSASYENDCEKIVRRVYPEVDYQLSWLLEYAPSRLTGTGAC
VFAEFINEKEAQHVFSLIPDNATGFITRGRNTSPLNQALEEYKSLCNI

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=280, Percent_Identity=56.0714285714286, Blast_Score=295, Evalue=3e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_ALISL (B6EHH2)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262297.1
- ProteinModelPortal:   B6EHH2
- GeneID:   6989016
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0787
- HOGENOM:   HBG734593
- OMA:   LNLFLHV
- ProtClustDB:   PRK00343
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31784; Mature: 31784

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: ACT_SITE 13-13 ACT_SITE 138-138

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITTATRWPSPAKLNLFLYINGQHDNGYHELQTLFQFIDLCDHLTITPNDSGEITLSPPI
CCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCEEECCCC
PGVEKKDNLIWKAATQLQEHTLCPLGAHIELEKILPIGGGIGGGSSNAATTLVALNFLWD
CCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHEEEEHHEE
LNLTNKELADLGVTLGADVPIFVHGFSAFAEGIGEKLQPANPKELWYVLIKPEVSIATVD
CCCCHHHHHHCCEECCCCCCEEEECHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEE
VFSHPELIRNTAKQPLNVLLSASYENDCEKIVRRVYPEVDYQLSWLLEYAPSRLTGTGAC
ECCCHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHH
VFAEFINEKEAQHVFSLIPDNATGFITRGRNTSPLNQALEEYKSLCNI
HHHHHHCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MITTATRWPSPAKLNLFLYINGQHDNGYHELQTLFQFIDLCDHLTITPNDSGEITLSPPI
CCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCEEECCCC
PGVEKKDNLIWKAATQLQEHTLCPLGAHIELEKILPIGGGIGGGSSNAATTLVALNFLWD
CCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHEEEEHHEE
LNLTNKELADLGVTLGADVPIFVHGFSAFAEGIGEKLQPANPKELWYVLIKPEVSIATVD
CCCCHHHHHHCCEECCCCCCEEEECHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEE
VFSHPELIRNTAKQPLNVLLSASYENDCEKIVRRVYPEVDYQLSWLLEYAPSRLTGTGAC
ECCCHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHH
VFAEFINEKEAQHVFSLIPDNATGFITRGRNTSPLNQALEEYKSLCNI
HHHHHHCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA