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The map label for this gene is prs [H]

Identifier: 209694368

GI number: 209694368

Start: 872539

End: 873537

Strand: Reverse

Name: prs [H]

Synonym: VSAL_I0786

Alternate gene names: 209694368

Gene position: 873537-872539 (Counterclockwise)

Preceding gene: 209694369

Following gene: 209694367

Centisome position: 26.27

GC content: 43.84

Gene sequence:

>999_bases
ATGCAACATATAAAATTCTCACAATTTGGACGCAACCCTGAGGTTTCCACCGTGCCTGACATGAAGCTATTTACTGGTAA
TGCAACTCCTGAACTTGCCCAACGTATCGCCGAACGACTATACATTTCACTTGGCGACGCTACTGTAGATCGTTTTTCTG
ACGGTGAAGTAGCCGTTCAAATTAACGAAAACGTACGTGGTAGTGATGTATTCATTATCCAGTCTACGTGTGCTCCTACT
AATGACAACATCATGGAATTAGTAGTAATGATCGATGCACTTCGCCGTGCTTCTGCAGGTCGTATTACTGCGGTAATTCC
TTACTTTGGCTATGCTCGTCAAGACCGTCGTGTACGTTCTGCTCGTGTACCAATCACTGCAAAAGTTATTGCTGATATGC
TTTCAAACGTTGGTGTTGACCGCGTTTTAACTATCGACTTACACGCTGAACAAATTCAAGGTTTCTTCGACGTACCAGTA
GATAACATCTTCGGTACTCCAGTATTACTTGATGATATGCGTACTCGTGGTTTAGAAAACCCTGTTGTTGTTTCTCCTGA
TTTAGGTGGTGTTGTACGTGCCCGTGCTACTGCTAAAGCATTAGGTGATGTAGACATCGCTATCGTTGATAAGCGTCGTC
CACGTGCTAACGTTTCAGAAGTAATGAACCTTATTGGTGATGTTGACGGCCGTGACTGTGTTATCGTTGATGACATGATC
GATACGGGCGGCACATTATGTAAAGCAGCTGAAGCACTAAAAGAACGCGGCGCAAAACGTGTATTCGCTTACGCAACTCA
CGCTGTTTTCTCTGGCACTGCTGCACATAACATTGCAAACTCAGTAATAGACCAAGTTGTTGTAACGGATTCAATCCCTC
TATCTAAAGAGATGGCAGCGACAGGCAAAGTAACTGAACTAAGCCTATCTCGTATGCTAGCTGAAGCAATTCGTCGCATT
AGCAACGAAGAGTCTATATCGGCAATGTTTGATCACTAA

Upstream 100 bases:

>100_bases
AACACGTTTTTTCATTAATTCCTGATAACGCAACTGGATTTATCACCCGAGGTAGAAATACTTCACCACTCAACCAAGCG
TTGGAAGAGTATAAATCATT

Downstream 100 bases:

>100_bases
TTTCAAACAAAAACAATATCCAAACGCCCTGCTATTGCGGGGCGTTTTTGTATTTGGAATTCTTGTGGTAATATGGCGCG
CCTTTTTAATGGTACATACT

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MQHIKFSQFGRNPEVSTVPDMKLFTGNATPELAQRIAERLYISLGDATVDRFSDGEVAVQINENVRGSDVFIIQSTCAPT
NDNIMELVVMIDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVIADMLSNVGVDRVLTIDLHAEQIQGFFDVPV
DNIFGTPVLLDDMRTRGLENPVVVSPDLGGVVRARATAKALGDVDIAIVDKRRPRANVSEVMNLIGDVDGRDCVIVDDMI
DTGGTLCKAAEALKERGAKRVFAYATHAVFSGTAAHNIANSVIDQVVVTDSIPLSKEMAATGKVTELSLSRMLAEAIRRI
SNEESISAMFDH

Sequences:

>Translated_332_residues
MQHIKFSQFGRNPEVSTVPDMKLFTGNATPELAQRIAERLYISLGDATVDRFSDGEVAVQINENVRGSDVFIIQSTCAPT
NDNIMELVVMIDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVIADMLSNVGVDRVLTIDLHAEQIQGFFDVPV
DNIFGTPVLLDDMRTRGLENPVVVSPDLGGVVRARATAKALGDVDIAIVDKRRPRANVSEVMNLIGDVDGRDCVIVDDMI
DTGGTLCKAAEALKERGAKRVFAYATHAVFSGTAAHNIANSVIDQVVVTDSIPLSKEMAATGKVTELSLSRMLAEAIRRI
SNEESISAMFDH
>Mature_332_residues
MQHIKFSQFGRNPEVSTVPDMKLFTGNATPELAQRIAERLYISLGDATVDRFSDGEVAVQINENVRGSDVFIIQSTCAPT
NDNIMELVVMIDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVIADMLSNVGVDRVLTIDLHAEQIQGFFDVPV
DNIFGTPVLLDDMRTRGLENPVVVSPDLGGVVRARATAKALGDVDIAIVDKRRPRANVSEVMNLIGDVDGRDCVIVDDMI
DTGGTLCKAAEALKERGAKRVFAYATHAVFSGTAAHNIANSVIDQVVVTDSIPLSKEMAATGKVTELSLSRMLAEAIRRI
SNEESISAMFDH

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=317, Percent_Identity=48.2649842271293, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI4506129, Length=317, Percent_Identity=47.6340694006309, Blast_Score=302, Evalue=2e-82,
Organism=Homo sapiens, GI84875539, Length=319, Percent_Identity=47.3354231974922, Blast_Score=301, Evalue=4e-82,
Organism=Homo sapiens, GI28557709, Length=317, Percent_Identity=46.6876971608833, Blast_Score=299, Evalue=2e-81,
Organism=Homo sapiens, GI4506133, Length=343, Percent_Identity=37.0262390670554, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI194018537, Length=343, Percent_Identity=36.4431486880466, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI310128524, Length=148, Percent_Identity=29.7297297297297, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI310115209, Length=148, Percent_Identity=29.7297297297297, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI310118259, Length=148, Percent_Identity=29.7297297297297, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI310119946, Length=148, Percent_Identity=29.7297297297297, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1787458, Length=315, Percent_Identity=81.5873015873016, Blast_Score=531, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI17554702, Length=317, Percent_Identity=46.3722397476341, Blast_Score=301, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI25149168, Length=317, Percent_Identity=46.3722397476341, Blast_Score=301, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI71989924, Length=317, Percent_Identity=46.3722397476341, Blast_Score=299, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17554704, Length=312, Percent_Identity=46.474358974359, Blast_Score=294, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI17570245, Length=339, Percent_Identity=34.5132743362832, Blast_Score=205, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6320946, Length=315, Percent_Identity=45.0793650793651, Blast_Score=273, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6319403, Length=316, Percent_Identity=44.3037974683544, Blast_Score=270, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6321776, Length=314, Percent_Identity=46.8152866242038, Blast_Score=266, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6322667, Length=207, Percent_Identity=42.0289855072464, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6324511, Length=111, Percent_Identity=43.2432432432432, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21355239, Length=319, Percent_Identity=46.7084639498433, Blast_Score=292, Evalue=3e-79,
Organism=Drosophila melanogaster, GI45551540, Length=341, Percent_Identity=43.6950146627566, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24651458, Length=360, Percent_Identity=32.7777777777778, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24651456, Length=360, Percent_Identity=32.7777777777778, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI281362873, Length=360, Percent_Identity=32.7777777777778, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24651454, Length=360, Percent_Identity=32.7777777777778, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI45552010, Length=378, Percent_Identity=32.2751322751323, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24651462, Length=378, Percent_Identity=32.2751322751323, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24651464, Length=378, Percent_Identity=32.2751322751323, Blast_Score=192, Evalue=2e-49,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 36084; Mature: 36084

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHIKFSQFGRNPEVSTVPDMKLFTGNATPELAQRIAERLYISLGDATVDRFSDGEVAVQ
CCCCCCHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHEEECCCHHHHCCCCCEEEEE
INENVRGSDVFIIQSTCAPTNDNIMELVVMIDALRRASAGRITAVIPYFGYARQDRRVRS
ECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHH
ARVPITAKVIADMLSNVGVDRVLTIDLHAEQIQGFFDVPVDNIFGTPVLLDDMRTRGLEN
HCCCCHHHHHHHHHHCCCCCEEEEEEEEHHHHCCEECCCHHHHCCCCEEEHHHHHCCCCC
PVVVSPDLGGVVRARATAKALGDVDIAIVDKRRPRANVSEVMNLIGDVDGRDCVIVDDMI
CEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEECCC
DTGGTLCKAAEALKERGAKRVFAYATHAVFSGTAAHNIANSVIDQVVVTDSIPLSKEMAA
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHC
TGKVTELSLSRMLAEAIRRISNEESISAMFDH
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCC
>Mature Secondary Structure
MQHIKFSQFGRNPEVSTVPDMKLFTGNATPELAQRIAERLYISLGDATVDRFSDGEVAVQ
CCCCCCHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHEEECCCHHHHCCCCCEEEEE
INENVRGSDVFIIQSTCAPTNDNIMELVVMIDALRRASAGRITAVIPYFGYARQDRRVRS
ECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHH
ARVPITAKVIADMLSNVGVDRVLTIDLHAEQIQGFFDVPVDNIFGTPVLLDDMRTRGLEN
HCCCCHHHHHHHHHHCCCCCEEEEEEEEHHHHCCEECCCHHHHCCCCEEEHHHHHCCCCC
PVVVSPDLGGVVRARATAKALGDVDIAIVDKRRPRANVSEVMNLIGDVDGRDCVIVDDMI
CEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEECCC
DTGGTLCKAAEALKERGAKRVFAYATHAVFSGTAAHNIANSVIDQVVVTDSIPLSKEMAA
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHC
TGKVTELSLSRMLAEAIRRISNEESISAMFDH
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]