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The map label for this gene is alaS [H]

Identifier: 209694226

GI number: 209694226

Start: 703629

End: 706211

Strand: Direct

Name: alaS [H]

Synonym: VSAL_I0635

Alternate gene names: 209694226

Gene position: 703629-706211 (Clockwise)

Preceding gene: 209694225

Following gene: 209694227

Centisome position: 21.16

GC content: 42.28

Gene sequence:

>2583_bases
ATGTATATGAGCACTGACGAAGTCCGCCGTGCATTCCTTGCTTTTTTTGAAAGCAAAGGGCACCAAATTGTTGAGAGTTC
TTCTCTAGTTCCAGCGAATGATCCAACTCTTTTATTTACGAATGCGGGTATGAACCAGTTTAAGGATTGTTTCCTTGGTT
CTGAAAAGCGAAGCTATACTCGTGCAACTACCGCTCAGCGTTGTGTTCGTGCCGGTGGTAAACATAACGATTTAGAAAAT
GTTGGTTTTACTGCTCGCCACCATACTTTCTTTGAAATGCTTGGTAACTTCAGCTTTGGTGATTACTTTAAGCAAGATGC
AATTAAGTATGCATGGGAATTCCTAACTGAGCGTTTAGAGCTTCCTGCGGATCGTCTGTTAGTGACAATCTACGAAACTG
ATGATGAAGCGTTTGAGATCTGGAATAAAGAAATGGGTATTCCAGCTGATCGTATCGTTCGTATTGGTGATAACAAAGGC
GCACCTTATGCATCAGATAACTTCTGGCAAATGGGTGACACGGGTCCTTGTGGTCCTTGTACTGAGATTTTTTACGATCA
CGGTGAACATATTTGGGGTGGCCGTCCAGGTACGCCTGAAGAAGATGGCGACCGTTTCATTGAAATCTGGAATAACGTTT
TCATGCAATTCAATCGCCTAGCTGATGGCACAATGGAACCACTTCCAAAACCATCAGTAGATACAGGGATGGGTATTGAG
CGTATCGCTGCAATTATGCAAGGTGTACATTCAAACTATGAAATTGATATCTTCCAAACACTAATTAAAGAAACGGCAAA
AGTTGTTGGTTATGATGATCTTTCAAACCAATCTTTACGTGTAGTTGCAGACCACATCCGTTCTTGTTCTTATTTAATCG
TTGATGGTGTTATGCCGTCAAACGAAGGTCGTGGCTATGTATTACGTCGTATTATTCGTCGTGCAGTACGTCACGGTAAC
AAGTTAGGTGCTAAAGGTTCTTACTTTTATAAGTTAGTTGGACCATTAGCTGAAATCATGGGTACGGCTGGTGAAGAGCT
TAAGAAGCAACAAGCACTAGTAGAAAAAGTACTTAAGATTGAAGAAGAGAACTTCGGCCGTACGCTTGAGCGTGGCATGG
TTATCTTAAACGAAGCGCTAGATAATCTAGAAGGCACCGTTCTTGACGGTGAAACTGTATTTAAACTTTATGATACTTAC
GGTTTCCCTGCTGACTTAACGAACGATGTTGCTCGTGAGCGTGAATTAACTATCGATGAAGATGGTTTTGAAAAAGCGAT
GAACGAACAACGCCAACGTGCTCGTGAAGCGGGTCAATTTGGTACTGATTATAATGCGGTTATCAAAGTTGAATCTGAAA
CGGTATTCCACGGTTATGATTTTACGGAAGCGAAAGCAACCGTATTAGAAATTTTCTGTGAAGGCGAAGCGGTTGAAACG
CTTTCTGCTGGCGATGATGCGATTCTAATCTTAGATGTAACACCGTTCTATGCTGAATCCGGTGGTCAATCTGGTGATGC
GGGTATTATTACCGTAGAAGCGGGTAAATTTGTTGTTACTGATACTCAAAAAGTAGGTAATGCGATTGGTCACCACGGTA
AATTAGTGGAAGGTGTATTATCTACGGCTGATAGAGCAATGGCTCAAGTGGATGAAGAGCGTCGCACTGCGATTACTTTA
AACCACTCGGCAACCCATTTATTACATGCAGCACTTCGTCAAGTACTGGGTGAGCATGTAACGCAAAAAGGGTCATTAGT
TAAAGCCGAAAGTTTACGTTTTGACTTTTCTAACCTTGAAGGGGTTAAACCGGAAGAACTTCGACTTGTTGAGCGTATTG
TTAACCAACAAGTACGTTTAAATCATGCAATTGAAACCGACCTTATGGACGTTGATGCGGCGAAAGAAAAAGGCGCAATG
GCTCTGTTTGGTGAGAAGTATGATGATGAAGTTCGTGTACTGTCAATGGGTGAGTTTTCAACTGAGCTATGTGGTGGTAT
TCACGCATCAAGTACGGGTGATATAGGTCTGTTTAAAATCACGTCTGAAGGTGGCATCGCCGCAGGTATTCGTCGTATTG
AAGCAGTAACTGGTGAAGCAGCATTAGATGCAATTGAATCTCAGCAGAAACTAGCGGATAAAAAGCTTCATGATGCGAGT
TCAAAAACGAAGTTACTTGAAAAAGAAATCCAACAATTGAAAGATAAATTGGCTTCTCAAGCAAGTTCAGACTTAATCAG
TCAAGTACAAGACATATCAGGCGTTAAAGTGTTGATCACTCAATTGGATGGTGCTGATAATAAAGCCCTTCGTGGTATGG
TTGATGAACTTAAAAACCAAATTGGCAGTGGTGTTATCATGATTGGTAATGTCAGTGATGATAAAGTGGGTTTAATTGCG
GGTGTGACTAAAGATCTTATTGGCCAAGTTAAAGCGGGCGAGTTAGTGAATATGGTTGCACTGCAAGTGGGTGGTAAAGG
CGGCGGCCGTCCTGATATGGCTCAAGCGGGCGGTACCGATGCAAAAGCATTACCTGCAGCGTTAGCGACGGTTGAAGCTT
GGTTAACTGAGCGTCTTTCATAG

Upstream 100 bases:

>100_bases
TCATTTGCGTATACAATATCCGAAATTCTGTCTTAAAAACACTGCTTGGCTTCAATGAGTACAAGAGTGAGTTAACTACG
ATTCAATCAGGAATATCCAC

Downstream 100 bases:

>100_bases
GGCGTATCAGTGGCATTAATCGTACAAAAGTATGGTGGAACATCAGTTGGGTCCGTTGAACGGATCCAACTTGTTTCTGA
CCGTATAATTTTAACGAAAC

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 860; Mature: 860

Protein sequence:

>860_residues
MYMSTDEVRRAFLAFFESKGHQIVESSSLVPANDPTLLFTNAGMNQFKDCFLGSEKRSYTRATTAQRCVRAGGKHNDLEN
VGFTARHHTFFEMLGNFSFGDYFKQDAIKYAWEFLTERLELPADRLLVTIYETDDEAFEIWNKEMGIPADRIVRIGDNKG
APYASDNFWQMGDTGPCGPCTEIFYDHGEHIWGGRPGTPEEDGDRFIEIWNNVFMQFNRLADGTMEPLPKPSVDTGMGIE
RIAAIMQGVHSNYEIDIFQTLIKETAKVVGYDDLSNQSLRVVADHIRSCSYLIVDGVMPSNEGRGYVLRRIIRRAVRHGN
KLGAKGSYFYKLVGPLAEIMGTAGEELKKQQALVEKVLKIEEENFGRTLERGMVILNEALDNLEGTVLDGETVFKLYDTY
GFPADLTNDVARERELTIDEDGFEKAMNEQRQRAREAGQFGTDYNAVIKVESETVFHGYDFTEAKATVLEIFCEGEAVET
LSAGDDAILILDVTPFYAESGGQSGDAGIITVEAGKFVVTDTQKVGNAIGHHGKLVEGVLSTADRAMAQVDEERRTAITL
NHSATHLLHAALRQVLGEHVTQKGSLVKAESLRFDFSNLEGVKPEELRLVERIVNQQVRLNHAIETDLMDVDAAKEKGAM
ALFGEKYDDEVRVLSMGEFSTELCGGIHASSTGDIGLFKITSEGGIAAGIRRIEAVTGEAALDAIESQQKLADKKLHDAS
SKTKLLEKEIQQLKDKLASQASSDLISQVQDISGVKVLITQLDGADNKALRGMVDELKNQIGSGVIMIGNVSDDKVGLIA
GVTKDLIGQVKAGELVNMVALQVGGKGGGRPDMAQAGGTDAKALPAALATVEAWLTERLS

Sequences:

>Translated_860_residues
MYMSTDEVRRAFLAFFESKGHQIVESSSLVPANDPTLLFTNAGMNQFKDCFLGSEKRSYTRATTAQRCVRAGGKHNDLEN
VGFTARHHTFFEMLGNFSFGDYFKQDAIKYAWEFLTERLELPADRLLVTIYETDDEAFEIWNKEMGIPADRIVRIGDNKG
APYASDNFWQMGDTGPCGPCTEIFYDHGEHIWGGRPGTPEEDGDRFIEIWNNVFMQFNRLADGTMEPLPKPSVDTGMGIE
RIAAIMQGVHSNYEIDIFQTLIKETAKVVGYDDLSNQSLRVVADHIRSCSYLIVDGVMPSNEGRGYVLRRIIRRAVRHGN
KLGAKGSYFYKLVGPLAEIMGTAGEELKKQQALVEKVLKIEEENFGRTLERGMVILNEALDNLEGTVLDGETVFKLYDTY
GFPADLTNDVARERELTIDEDGFEKAMNEQRQRAREAGQFGTDYNAVIKVESETVFHGYDFTEAKATVLEIFCEGEAVET
LSAGDDAILILDVTPFYAESGGQSGDAGIITVEAGKFVVTDTQKVGNAIGHHGKLVEGVLSTADRAMAQVDEERRTAITL
NHSATHLLHAALRQVLGEHVTQKGSLVKAESLRFDFSNLEGVKPEELRLVERIVNQQVRLNHAIETDLMDVDAAKEKGAM
ALFGEKYDDEVRVLSMGEFSTELCGGIHASSTGDIGLFKITSEGGIAAGIRRIEAVTGEAALDAIESQQKLADKKLHDAS
SKTKLLEKEIQQLKDKLASQASSDLISQVQDISGVKVLITQLDGADNKALRGMVDELKNQIGSGVIMIGNVSDDKVGLIA
GVTKDLIGQVKAGELVNMVALQVGGKGGGRPDMAQAGGTDAKALPAALATVEAWLTERLS
>Mature_860_residues
MYMSTDEVRRAFLAFFESKGHQIVESSSLVPANDPTLLFTNAGMNQFKDCFLGSEKRSYTRATTAQRCVRAGGKHNDLEN
VGFTARHHTFFEMLGNFSFGDYFKQDAIKYAWEFLTERLELPADRLLVTIYETDDEAFEIWNKEMGIPADRIVRIGDNKG
APYASDNFWQMGDTGPCGPCTEIFYDHGEHIWGGRPGTPEEDGDRFIEIWNNVFMQFNRLADGTMEPLPKPSVDTGMGIE
RIAAIMQGVHSNYEIDIFQTLIKETAKVVGYDDLSNQSLRVVADHIRSCSYLIVDGVMPSNEGRGYVLRRIIRRAVRHGN
KLGAKGSYFYKLVGPLAEIMGTAGEELKKQQALVEKVLKIEEENFGRTLERGMVILNEALDNLEGTVLDGETVFKLYDTY
GFPADLTNDVARERELTIDEDGFEKAMNEQRQRAREAGQFGTDYNAVIKVESETVFHGYDFTEAKATVLEIFCEGEAVET
LSAGDDAILILDVTPFYAESGGQSGDAGIITVEAGKFVVTDTQKVGNAIGHHGKLVEGVLSTADRAMAQVDEERRTAITL
NHSATHLLHAALRQVLGEHVTQKGSLVKAESLRFDFSNLEGVKPEELRLVERIVNQQVRLNHAIETDLMDVDAAKEKGAM
ALFGEKYDDEVRVLSMGEFSTELCGGIHASSTGDIGLFKITSEGGIAAGIRRIEAVTGEAALDAIESQQKLADKKLHDAS
SKTKLLEKEIQQLKDKLASQASSDLISQVQDISGVKVLITQLDGADNKALRGMVDELKNQIGSGVIMIGNVSDDKVGLIA
GVTKDLIGQVKAGELVNMVALQVGGKGGGRPDMAQAGGTDAKALPAALATVEAWLTERLS

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=980, Percent_Identity=36.0204081632653, Blast_Score=521, Evalue=1e-148,
Organism=Homo sapiens, GI38569417, Length=927, Percent_Identity=34.8435814455232, Blast_Score=453, Evalue=1e-127,
Organism=Escherichia coli, GI1789048, Length=876, Percent_Identity=67.4657534246575, Blast_Score=1219, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=975, Percent_Identity=37.1282051282051, Blast_Score=544, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI17536681, Length=743, Percent_Identity=34.3203230148048, Blast_Score=402, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324911, Length=837, Percent_Identity=41.3381123058542, Blast_Score=571, Evalue=1e-163,
Organism=Drosophila melanogaster, GI24582809, Length=976, Percent_Identity=36.577868852459, Blast_Score=555, Evalue=1e-158,
Organism=Drosophila melanogaster, GI45552267, Length=976, Percent_Identity=36.577868852459, Blast_Score=555, Evalue=1e-158,
Organism=Drosophila melanogaster, GI24658214, Length=852, Percent_Identity=30.5164319248826, Blast_Score=339, Evalue=5e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 94298; Mature: 94298

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYMSTDEVRRAFLAFFESKGHQIVESSSLVPANDPTLLFTNAGMNQFKDCFLGSEKRSYT
CCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEECCCCHHHHHHHCCCCHHHHH
RATTAQRCVRAGGKHNDLENVGFTARHHTFFEMLGNFSFGDYFKQDAIKYAWEFLTERLE
HHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LPADRLLVTIYETDDEAFEIWNKEMGIPADRIVRIGDNKGAPYASDNFWQMGDTGPCGPC
CCCCCEEEEEEECCCHHHHHHHHCCCCCHHHEEEECCCCCCCCCCCCCEECCCCCCCCHH
TEIFYDHGEHIWGGRPGTPEEDGDRFIEIWNNVFMQFNRLADGTMEPLPKPSVDTGMGIE
HHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
RIAAIMQGVHSNYEIDIFQTLIKETAKVVGYDDLSNQSLRVVADHIRSCSYLIVDGVMPS
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCC
NEGRGYVLRRIIRRAVRHGNKLGAKGSYFYKLVGPLAEIMGTAGEELKKQQALVEKVLKI
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EEENFGRTLERGMVILNEALDNLEGTVLDGETVFKLYDTYGFPADLTNDVARERELTIDE
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
DGFEKAMNEQRQRAREAGQFGTDYNAVIKVESETVFHGYDFTEAKATVLEIFCEGEAVET
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEECCCCCHHHHHHHHHHCCCCHHHH
LSAGDDAILILDVTPFYAESGGQSGDAGIITVEAGKFVVTDTQKVGNAIGHHGKLVEGVL
CCCCCCEEEEEECCHHHHHCCCCCCCCEEEEEECCCEEEECHHHHHHHHCCCHHHHHHHH
STADRAMAQVDEERRTAITLNHSATHLLHAALRQVLGEHVTQKGSLVKAESLRFDFSNLE
HHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHEEEHHCCC
GVKPEELRLVERIVNQQVRLNHAIETDLMDVDAAKEKGAMALFGEKYDDEVRVLSMGEFS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEECCCCCCCEEEEECCCHH
TELCGGIHASSTGDIGLFKITSEGGIAAGIRRIEAVTGEAALDAIESQQKLADKKLHDAS
HHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SKTKLLEKEIQQLKDKLASQASSDLISQVQDISGVKVLITQLDGADNKALRGMVDELKNQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
IGSGVIMIGNVSDDKVGLIAGVTKDLIGQVKAGELVNMVALQVGGKGGGRPDMAQAGGTD
HCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHEECCCCCCCCCHHHCCCCC
AKALPAALATVEAWLTERLS
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYMSTDEVRRAFLAFFESKGHQIVESSSLVPANDPTLLFTNAGMNQFKDCFLGSEKRSYT
CCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEECCCCHHHHHHHCCCCHHHHH
RATTAQRCVRAGGKHNDLENVGFTARHHTFFEMLGNFSFGDYFKQDAIKYAWEFLTERLE
HHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LPADRLLVTIYETDDEAFEIWNKEMGIPADRIVRIGDNKGAPYASDNFWQMGDTGPCGPC
CCCCCEEEEEEECCCHHHHHHHHCCCCCHHHEEEECCCCCCCCCCCCCEECCCCCCCCHH
TEIFYDHGEHIWGGRPGTPEEDGDRFIEIWNNVFMQFNRLADGTMEPLPKPSVDTGMGIE
HHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
RIAAIMQGVHSNYEIDIFQTLIKETAKVVGYDDLSNQSLRVVADHIRSCSYLIVDGVMPS
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCC
NEGRGYVLRRIIRRAVRHGNKLGAKGSYFYKLVGPLAEIMGTAGEELKKQQALVEKVLKI
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EEENFGRTLERGMVILNEALDNLEGTVLDGETVFKLYDTYGFPADLTNDVARERELTIDE
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
DGFEKAMNEQRQRAREAGQFGTDYNAVIKVESETVFHGYDFTEAKATVLEIFCEGEAVET
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEECCCCCHHHHHHHHHHCCCCHHHH
LSAGDDAILILDVTPFYAESGGQSGDAGIITVEAGKFVVTDTQKVGNAIGHHGKLVEGVL
CCCCCCEEEEEECCHHHHHCCCCCCCCEEEEEECCCEEEECHHHHHHHHCCCHHHHHHHH
STADRAMAQVDEERRTAITLNHSATHLLHAALRQVLGEHVTQKGSLVKAESLRFDFSNLE
HHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHEEEHHCCC
GVKPEELRLVERIVNQQVRLNHAIETDLMDVDAAKEKGAMALFGEKYDDEVRVLSMGEFS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEECCCCCCCEEEEECCCHH
TELCGGIHASSTGDIGLFKITSEGGIAAGIRRIEAVTGEAALDAIESQQKLADKKLHDAS
HHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SKTKLLEKEIQQLKDKLASQASSDLISQVQDISGVKVLITQLDGADNKALRGMVDELKNQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
IGSGVIMIGNVSDDKVGLIAGVTKDLIGQVKAGELVNMVALQVGGKGGGRPDMAQAGGTD
HCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHEECCCCCCCCCHHHCCCCC
AKALPAALATVEAWLTERLS
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA