Click here to switch to the map view.

The map label for this gene is dusA [H]

Identifier: 209694000

GI number: 209694000

Start: 437014

End: 438030

Strand: Direct

Name: dusA [H]

Synonym: VSAL_I0399

Alternate gene names: 209694000

Gene position: 437014-438030 (Clockwise)

Preceding gene: 209693996

Following gene: 209694001

Centisome position: 13.14

GC content: 41.59

Gene sequence:

>1017_bases
ATGGCTAACACTTTGAATAGTAAAGAGATTTTAACTATGCATCCACTACAACGCTTTTCTGTCGCTCCAATGCTTGATTG
GACTGACCGCCATTGCCGCTATTTTCATCGACAATTATCTGAGCATGCTCTTTTATATTCCGAGATGATCACGACGGGTG
CCATTATTCATGGGAAAGGTGATTTCCTTGCTTACAATGAAGAAGAGCATCCATTAGCACTGCAATTAGGGGGCTCAAAC
GCGAAAGATTTAGCACATTGTGCGAAATTAGCGGCAGATCGTGGTTATGATGAAATAAACTTGAATGTAGGTTGTCCATC
AGATCGTGTTCAAAACGGTCGTTTCGGTGCTTGTTTGATGGCCGATCCAGAATTAGTGGCTGAATGTATTGCTGAAATGA
AAGCGGTTGTCGATATTCCAGTGACAGTTAAAACACGCATAGGTATTGATGATCAAGATAGCTATGAGTTTTTAACTAAA
TTTGTATCAACGGTCCATGAAAAAGGCGGTTGTGACCAATTTACCATCCATGCGCGTAAAGCGTGGCTGAGTGGATTAAG
CCCTAAAGAAAACCGTGAGATCCCACCGCTTGATTACCCTCGAGCGTATCAAATTAAGAAAGATTTCCCTCAACTGATCA
TTGCTGTGAATGGTGGAATTAAAACACTTGAAGAAGCAAAAGAACATTTAGCGCATTTAGATGGTGTAATGATTGGTCGC
GAAGCGTATCAAAGCCCATACTTATTAGCGGAAGTTGATCAACAGTTGTTTGGTAGCGATAAACCAATTAAAAAACGCCG
TGAAATTGTTGAATCAATGTATCCGTATATTGAGCGTCAATTGGAGAATGGGGCTCATTTAGGCCACATTACTCGTCATA
TGCTTGGGTTATTTCAAAGTATGCCAGGTGCAAGACAATGGCGACGTTACATCAGTGAAAATGCACACAAACCAGGCTCA
GGTATTGAAGTGGTTCAAGCCGCTCTTGCTAAAATTCCAGCCGAACTGGATGTGTAA

Upstream 100 bases:

>100_bases
GCGTGTCTTATTTGTCCCCAAAAACATCCCTTAGGGGACATTTGAGGTAATAAACTCGATAATATCGCTCGTTTTATACC
GTTTTGCCGTAATAGAATGT

Downstream 100 bases:

>100_bases
GCTTTTGATATTGAGCTGATAAGTTTTATATAGAAAGAAAAAGAAGAGTACATAAATAAACCAAATCGTTTATTATGACC
GCAGATGAACCAAATAGCGT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MANTLNSKEILTMHPLQRFSVAPMLDWTDRHCRYFHRQLSEHALLYSEMITTGAIIHGKGDFLAYNEEEHPLALQLGGSN
AKDLAHCAKLAADRGYDEINLNVGCPSDRVQNGRFGACLMADPELVAECIAEMKAVVDIPVTVKTRIGIDDQDSYEFLTK
FVSTVHEKGGCDQFTIHARKAWLSGLSPKENREIPPLDYPRAYQIKKDFPQLIIAVNGGIKTLEEAKEHLAHLDGVMIGR
EAYQSPYLLAEVDQQLFGSDKPIKKRREIVESMYPYIERQLENGAHLGHITRHMLGLFQSMPGARQWRRYISENAHKPGS
GIEVVQAALAKIPAELDV

Sequences:

>Translated_338_residues
MANTLNSKEILTMHPLQRFSVAPMLDWTDRHCRYFHRQLSEHALLYSEMITTGAIIHGKGDFLAYNEEEHPLALQLGGSN
AKDLAHCAKLAADRGYDEINLNVGCPSDRVQNGRFGACLMADPELVAECIAEMKAVVDIPVTVKTRIGIDDQDSYEFLTK
FVSTVHEKGGCDQFTIHARKAWLSGLSPKENREIPPLDYPRAYQIKKDFPQLIIAVNGGIKTLEEAKEHLAHLDGVMIGR
EAYQSPYLLAEVDQQLFGSDKPIKKRREIVESMYPYIERQLENGAHLGHITRHMLGLFQSMPGARQWRRYISENAHKPGS
GIEVVQAALAKIPAELDV
>Mature_337_residues
ANTLNSKEILTMHPLQRFSVAPMLDWTDRHCRYFHRQLSEHALLYSEMITTGAIIHGKGDFLAYNEEEHPLALQLGGSNA
KDLAHCAKLAADRGYDEINLNVGCPSDRVQNGRFGACLMADPELVAECIAEMKAVVDIPVTVKTRIGIDDQDSYEFLTKF
VSTVHEKGGCDQFTIHARKAWLSGLSPKENREIPPLDYPRAYQIKKDFPQLIIAVNGGIKTLEEAKEHLAHLDGVMIGRE
AYQSPYLLAEVDQQLFGSDKPIKKRREIVESMYPYIERQLENGAHLGHITRHMLGLFQSMPGARQWRRYISENAHKPGSG
IEVVQAALAKIPAELDV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=309, Percent_Identity=25.5663430420712, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI145693211, Length=321, Percent_Identity=71.0280373831776, Blast_Score=493, Evalue=1e-141,
Organism=Escherichia coli, GI1789660, Length=246, Percent_Identity=26.8292682926829, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788462, Length=243, Percent_Identity=26.3374485596708, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25144369, Length=194, Percent_Identity=29.8969072164948, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24580595, Length=244, Percent_Identity=26.2295081967213, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920448, Length=244, Percent_Identity=26.2295081967213, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37971; Mature: 37840

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANTLNSKEILTMHPLQRFSVAPMLDWTDRHCRYFHRQLSEHALLYSEMITTGAIIHGKG
CCCCCCCCCEEEECCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCC
DFLAYNEEEHPLALQLGGSNAKDLAHCAKLAADRGYDEINLNVGCPSDRVQNGRFGACLM
CEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEEE
ADPELVAECIAEMKAVVDIPVTVKTRIGIDDQDSYEFLTKFVSTVHEKGGCDQFTIHARK
CCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHH
AWLSGLSPKENREIPPLDYPRAYQIKKDFPQLIIAVNGGIKTLEEAKEHLAHLDGVMIGR
HHHCCCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCH
EAYQSPYLLAEVDQQLFGSDKPIKKRREIVESMYPYIERQLENGAHLGHITRHMLGLFQS
HHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
MPGARQWRRYISENAHKPGSGIEVVQAALAKIPAELDV
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ANTLNSKEILTMHPLQRFSVAPMLDWTDRHCRYFHRQLSEHALLYSEMITTGAIIHGKG
CCCCCCCCEEEECCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCC
DFLAYNEEEHPLALQLGGSNAKDLAHCAKLAADRGYDEINLNVGCPSDRVQNGRFGACLM
CEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEEE
ADPELVAECIAEMKAVVDIPVTVKTRIGIDDQDSYEFLTKFVSTVHEKGGCDQFTIHARK
CCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHH
AWLSGLSPKENREIPPLDYPRAYQIKKDFPQLIIAVNGGIKTLEEAKEHLAHLDGVMIGR
HHHCCCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCH
EAYQSPYLLAEVDQQLFGSDKPIKKRREIVESMYPYIERQLENGAHLGHITRHMLGLFQS
HHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
MPGARQWRRYISENAHKPGSGIEVVQAALAKIPAELDV
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]