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The map label for this gene is leuB [H]

Identifier: 209693979

GI number: 209693979

Start: 411849

End: 412940

Strand: Reverse

Name: leuB [H]

Synonym: VSAL_I0376

Alternate gene names: 209693979

Gene position: 412940-411849 (Counterclockwise)

Preceding gene: 209693980

Following gene: 209693978

Centisome position: 12.42

GC content: 43.13

Gene sequence:

>1092_bases
ATGACGAATAAAACATACAAGATTGCGATTTTACCTGGCGATGGTATCGGCCCAGAAGTCATGGACCAAGCACATAAAGT
TCTGGATGCGATTGAGAAAAAACACGCGATTTCATTTTCTCGTGAACAACATGATGTGGGTGGAATCGCGATAGATAATC
ACGGTTGTCCACTACCAGAAAGTACCGTAAAAGGCTGTGAAGAATCAGATGCGGTTCTATTCGGCTCCGTTGGTGGACCT
AAATGGGAAAACCTTCCACCGAATGATCAACCAGAACGTGGCGCCCTACTTCCTCTTCGTAAGCATTTCGAACTGTTTTG
TAACCTACGTCCTGCTCAAATTCATAAAGGATTAGAAGGGTTTTCTCCACTGCGTGCTGATATTTCGGGTAACGGTTTTG
ATATCGTTGTTGTTCGTGAACTGACTGGTGGCATTTACTTTGGTCAACCGAAAGGTCGTGAAGGCGAAGGTCCTCAAGAG
AAAGCGTACGATACTGAAATTTATCATCGTTATGAAATTGAACGTATTGCAAAGATTGCTTTTGAATCTGCGCTTCTACG
TAACAAAAACGTGTACTCAATTGATAAAGCCAACGTTCTACAAAGCTCTATTTTATGGCGTGAAGTGGTCGAAGAAGTAG
CGAAAGGCTACCCAGAAGTCAAACTGAGCCACATGTACATTGATAATGCGACCATGCAACTAATCAAAGATCCCTCTCAG
TTTGATGTCATGCTGTGTTCAAATATCTTTGGGGACATCATTTCTGATGAATGTGCCATGATCACAGGCTCTATGGGCAT
GCTTCCTTCAGCAAGCCTAAATAAAGATAACTTTGGTTTGTATGAGCCAGCAGGTGGTTCAGCACCAGATATCGCAGGCA
AAAACATTGCAAATCCAGTCGCTCAAATTTTATCGGCGGCATTGATGTTACGCTATAGCCTTGGTGAAGAAGAAGCAGCT
CAAAGTATTGAAGCTGCCGTATCTAAAGCGCTATCAGCAGGTGAACTAACGGCCGATCTTGCCGGAAATAAACCCGCATT
AACGACCTCAGCAATGGGCGATAAAATCGCTGAATATATTTTGAATTCGTAA

Upstream 100 bases:

>100_bases
AAAATAAAATTACGACGGTATAGCACTAAATAAATTTAAATCAGGGCAAATACACCGTGCCTTGATTTTCCAAATTAAAT
AGAAAGATTAAAGGATTAAC

Downstream 100 bases:

>100_bases
TTCAAGGAAGAAGCAATGTCTAACGCAAAAACATTATACGAAAAAGTTTATGATGCCCATGTTGCAGTTGCTGCAAAAGG
TGAAACCCCAATTTTATATA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTNKTYKIAILPGDGIGPEVMDQAHKVLDAIEKKHAISFSREQHDVGGIAIDNHGCPLPESTVKGCEESDAVLFGSVGGP
KWENLPPNDQPERGALLPLRKHFELFCNLRPAQIHKGLEGFSPLRADISGNGFDIVVVRELTGGIYFGQPKGREGEGPQE
KAYDTEIYHRYEIERIAKIAFESALLRNKNVYSIDKANVLQSSILWREVVEEVAKGYPEVKLSHMYIDNATMQLIKDPSQ
FDVMLCSNIFGDIISDECAMITGSMGMLPSASLNKDNFGLYEPAGGSAPDIAGKNIANPVAQILSAALMLRYSLGEEEAA
QSIEAAVSKALSAGELTADLAGNKPALTTSAMGDKIAEYILNS

Sequences:

>Translated_363_residues
MTNKTYKIAILPGDGIGPEVMDQAHKVLDAIEKKHAISFSREQHDVGGIAIDNHGCPLPESTVKGCEESDAVLFGSVGGP
KWENLPPNDQPERGALLPLRKHFELFCNLRPAQIHKGLEGFSPLRADISGNGFDIVVVRELTGGIYFGQPKGREGEGPQE
KAYDTEIYHRYEIERIAKIAFESALLRNKNVYSIDKANVLQSSILWREVVEEVAKGYPEVKLSHMYIDNATMQLIKDPSQ
FDVMLCSNIFGDIISDECAMITGSMGMLPSASLNKDNFGLYEPAGGSAPDIAGKNIANPVAQILSAALMLRYSLGEEEAA
QSIEAAVSKALSAGELTADLAGNKPALTTSAMGDKIAEYILNS
>Mature_362_residues
TNKTYKIAILPGDGIGPEVMDQAHKVLDAIEKKHAISFSREQHDVGGIAIDNHGCPLPESTVKGCEESDAVLFGSVGGPK
WENLPPNDQPERGALLPLRKHFELFCNLRPAQIHKGLEGFSPLRADISGNGFDIVVVRELTGGIYFGQPKGREGEGPQEK
AYDTEIYHRYEIERIAKIAFESALLRNKNVYSIDKANVLQSSILWREVVEEVAKGYPEVKLSHMYIDNATMQLIKDPSQF
DVMLCSNIFGDIISDECAMITGSMGMLPSASLNKDNFGLYEPAGGSAPDIAGKNIANPVAQILSAALMLRYSLGEEEAAQ
SIEAAVSKALSAGELTADLAGNKPALTTSAMGDKIAEYILNS

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=346, Percent_Identity=33.8150289017341, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI28178816, Length=347, Percent_Identity=29.3948126801153, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI28178821, Length=347, Percent_Identity=29.3948126801153, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI4758582, Length=362, Percent_Identity=29.5580110497238, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI28178838, Length=332, Percent_Identity=30.1204819277108, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI28178819, Length=181, Percent_Identity=34.8066298342541, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI87081683, Length=358, Percent_Identity=72.9050279329609, Blast_Score=552, Evalue=1e-158,
Organism=Escherichia coli, GI1788101, Length=370, Percent_Identity=37.027027027027, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1787381, Length=404, Percent_Identity=25.7425742574257, Blast_Score=88, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI71986051, Length=340, Percent_Identity=32.3529411764706, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17550882, Length=350, Percent_Identity=32.2857142857143, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17505779, Length=353, Percent_Identity=31.1614730878187, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI25144293, Length=343, Percent_Identity=28.8629737609329, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=365, Percent_Identity=46.027397260274, Blast_Score=285, Evalue=7e-78,
Organism=Saccharomyces cerevisiae, GI6322097, Length=353, Percent_Identity=36.2606232294618, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6324709, Length=362, Percent_Identity=30.6629834254144, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6324291, Length=345, Percent_Identity=30.4347826086957, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24643270, Length=341, Percent_Identity=31.9648093841642, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24643268, Length=341, Percent_Identity=31.9648093841642, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24661184, Length=344, Percent_Identity=31.1046511627907, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI161078637, Length=349, Percent_Identity=29.7994269340974, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161078633, Length=345, Percent_Identity=30.1449275362319, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24650122, Length=345, Percent_Identity=30.1449275362319, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161078635, Length=345, Percent_Identity=30.1449275362319, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161078639, Length=345, Percent_Identity=30.1449275362319, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI281362242, Length=345, Percent_Identity=27.8260869565217, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648872, Length=345, Percent_Identity=27.8260869565217, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI20130355, Length=351, Percent_Identity=23.6467236467236, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39294; Mature: 39163

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKTYKIAILPGDGIGPEVMDQAHKVLDAIEKKHAISFSREQHDVGGIAIDNHGCPLPE
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCH
STVKGCEESDAVLFGSVGGPKWENLPPNDQPERGALLPLRKHFELFCNLRPAQIHKGLEG
HHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCC
FSPLRADISGNGFDIVVVRELTGGIYFGQPKGREGEGPQEKAYDTEIYHRYEIERIAKIA
CCCCCCCCCCCCEEEEEEEECCCCEEECCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHH
FESALLRNKNVYSIDKANVLQSSILWREVVEEVAKGYPEVKLSHMYIDNATMQLIKDPSQ
HHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHCCCCH
FDVMLCSNIFGDIISDECAMITGSMGMLPSASLNKDNFGLYEPAGGSAPDIAGKNIANPV
HHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
AQILSAALMLRYSLGEEEAAQSIEAAVSKALSAGELTADLAGNKPALTTSAMGDKIAEYI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHH
LNS
HCC
>Mature Secondary Structure 
TNKTYKIAILPGDGIGPEVMDQAHKVLDAIEKKHAISFSREQHDVGGIAIDNHGCPLPE
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCH
STVKGCEESDAVLFGSVGGPKWENLPPNDQPERGALLPLRKHFELFCNLRPAQIHKGLEG
HHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCC
FSPLRADISGNGFDIVVVRELTGGIYFGQPKGREGEGPQEKAYDTEIYHRYEIERIAKIA
CCCCCCCCCCCCEEEEEEEECCCCEEECCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHH
FESALLRNKNVYSIDKANVLQSSILWREVVEEVAKGYPEVKLSHMYIDNATMQLIKDPSQ
HHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHCCCCH
FDVMLCSNIFGDIISDECAMITGSMGMLPSASLNKDNFGLYEPAGGSAPDIAGKNIANPV
HHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
AQILSAALMLRYSLGEEEAAQSIEAAVSKALSAGELTADLAGNKPALTTSAMGDKIAEYI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHH
LNS
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA