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The map label for this gene is leuC

Identifier: 209693978

GI number: 209693978

Start: 410418

End: 411833

Strand: Reverse

Name: leuC

Synonym: VSAL_I0375

Alternate gene names: 209693978

Gene position: 411833-410418 (Counterclockwise)

Preceding gene: 209693979

Following gene: 209693977

Centisome position: 12.39

GC content: 44.92

Gene sequence:

>1416_bases
ATGTCTAACGCAAAAACATTATACGAAAAAGTTTATGATGCCCATGTTGCAGTTGCTGCAAAAGGTGAAACCCCAATTTT
ATATATCGACCGCCATTTAGTTCATGAAGTGACCTCACCACAAGCGTTTGATGGTCTGCGTGAAAAAGGTCGTAACGTTC
GCCAAGTCGGTAAAACTTTCGCGACGATGGATCACAACGTATCAACACAAACCAAAGACATTAATGCCTCTGGTGAAATG
GCTCGAATCCAAATGGAAACACTGTCTAAAAACTGTGAAGAATTTGGCGTAACCCTTTACGATCTAAACCATAAATACCA
AGGTATTGTGCACGTAATGGGACCAGAGCTAGGTATTACACTTCCAGGTATGACGATTGTGTGTGGAGATTCACACACCG
CAACGCACGGCGCATTTGGTTCTCTTGCATTTGGTATTGGTACCTCTGAAGTTGAACACGTATTAGCGACACAAACGCTA
AAGCAAGCTCGTGCAAAAACGATGAAGATCGAAGTTAAGGGAAAAGTCACAGAAGGCATTACCGCAAAAGACATCGTTCT
AGCGATCATTGGTAAAACAACCGCAGCTGGCGGTACTGGTTATGTGGTTGAATTCTGTGGTGAGGCAATTACAGATCTGA
CGATGGAAGGTCGCATGACGGTATGTAATATGGCGATAGAGCTAGGTGCCAAAGCCGGCCTAATCGCACCAGATGCCACC
ACGTTTGACTACATTACTGGTCGTAAATTCTCACCTCAAGGTGAAGATTTGACGGCAGCTATCGAATATTGGTCGTCACT
AAAAACGGATGATGATGCAAAGTTTGATGCCGTTGTTAGCCTTGATGCCTCTGAAATTAAACCACAAGTGACGTGGGGAA
CAAACCCTGGCCAAGTTATTGCCATTGATCAACCCATTCCTGCACCAGAGAGTTTTTCTGACCCGGTAGAAAAAGTATCC
GCAGAAAAAGCCTTGGCTTATATGGGTCTTGAAGCCGGTAAATCATTAACAGATTACAACGTAGACAAAGTGTTTGTCGG
CTCTTGTACCAACTCTCGTATTGAAGATATCCGCGCTGCTGCTGCTGTTGCTAAAGGCAACAAAGTCGCCGCTCATGTTC
AAGCCTTGATTGTTCCAGGTTCTGAGCAAGTAAAAGCACAAGCGGAAAAAGAAGGATTAGACATCATCTTTAAAGAAGCG
GGTTTTGAGTGGCGTTTACCGGGTTGCTCTATGTGTCTTGCAATGAACAATGACCGTTTAGGTCCACAAGAACGTTGCGC
ATCAACATCAAACCGTAACTTTGAAGGTCGCCAAGGCCGTGATGGACGAACTCACCTAGTAAGCCCAGCCATGGCAGCGG
CAGCCGCGATTGCGGGTCATTTTGTTGATATTCGTACCATCACAGAAAAAGCATAA

Upstream 100 bases:

>100_bases
AACGGCCGATCTTGCCGGAAATAAACCCGCATTAACGACCTCAGCAATGGGCGATAAAATCGCTGAATATATTTTGAATT
CGTAATTCAAGGAAGAAGCA

Downstream 100 bases:

>100_bases
AGGAATTTACTATGTCAGGTTTTAAACAACATACAGGTCTAGTCGTTCCTTTAGATACGGCAAACATTGATACCGATGCC
ATCATTCCAAAACAGTTTTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSNAKTLYEKVYDAHVAVAAKGETPILYIDRHLVHEVTSPQAFDGLREKGRNVRQVGKTFATMDHNVSTQTKDINASGEM
ARIQMETLSKNCEEFGVTLYDLNHKYQGIVHVMGPELGITLPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTL
KQARAKTMKIEVKGKVTEGITAKDIVLAIIGKTTAAGGTGYVVEFCGEAITDLTMEGRMTVCNMAIELGAKAGLIAPDAT
TFDYITGRKFSPQGEDLTAAIEYWSSLKTDDDAKFDAVVSLDASEIKPQVTWGTNPGQVIAIDQPIPAPESFSDPVEKVS
AEKALAYMGLEAGKSLTDYNVDKVFVGSCTNSRIEDIRAAAAVAKGNKVAAHVQALIVPGSEQVKAQAEKEGLDIIFKEA
GFEWRLPGCSMCLAMNNDRLGPQERCASTSNRNFEGRQGRDGRTHLVSPAMAAAAAIAGHFVDIRTITEKA

Sequences:

>Translated_471_residues
MSNAKTLYEKVYDAHVAVAAKGETPILYIDRHLVHEVTSPQAFDGLREKGRNVRQVGKTFATMDHNVSTQTKDINASGEM
ARIQMETLSKNCEEFGVTLYDLNHKYQGIVHVMGPELGITLPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTL
KQARAKTMKIEVKGKVTEGITAKDIVLAIIGKTTAAGGTGYVVEFCGEAITDLTMEGRMTVCNMAIELGAKAGLIAPDAT
TFDYITGRKFSPQGEDLTAAIEYWSSLKTDDDAKFDAVVSLDASEIKPQVTWGTNPGQVIAIDQPIPAPESFSDPVEKVS
AEKALAYMGLEAGKSLTDYNVDKVFVGSCTNSRIEDIRAAAAVAKGNKVAAHVQALIVPGSEQVKAQAEKEGLDIIFKEA
GFEWRLPGCSMCLAMNNDRLGPQERCASTSNRNFEGRQGRDGRTHLVSPAMAAAAAIAGHFVDIRTITEKA
>Mature_470_residues
SNAKTLYEKVYDAHVAVAAKGETPILYIDRHLVHEVTSPQAFDGLREKGRNVRQVGKTFATMDHNVSTQTKDINASGEMA
RIQMETLSKNCEEFGVTLYDLNHKYQGIVHVMGPELGITLPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTLK
QARAKTMKIEVKGKVTEGITAKDIVLAIIGKTTAAGGTGYVVEFCGEAITDLTMEGRMTVCNMAIELGAKAGLIAPDATT
FDYITGRKFSPQGEDLTAAIEYWSSLKTDDDAKFDAVVSLDASEIKPQVTWGTNPGQVIAIDQPIPAPESFSDPVEKVSA
EKALAYMGLEAGKSLTDYNVDKVFVGSCTNSRIEDIRAAAAVAKGNKVAAHVQALIVPGSEQVKAQAEKEGLDIIFKEAG
FEWRLPGCSMCLAMNNDRLGPQERCASTSNRNFEGRQGRDGRTHLVSPAMAAAAAIAGHFVDIRTITEKA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=481, Percent_Identity=26.4033264033264, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI8659555, Length=367, Percent_Identity=26.7029972752044, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI41352693, Length=375, Percent_Identity=26.1333333333333, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=78.8793103448276, Blast_Score=759, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=362, Percent_Identity=28.4530386740331, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI87081781, Length=360, Percent_Identity=25.2777777777778, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI2367097, Length=350, Percent_Identity=24.5714285714286, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=420, Percent_Identity=27.6190476190476, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI32564738, Length=420, Percent_Identity=27.6190476190476, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI25149342, Length=315, Percent_Identity=29.2063492063492, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17568399, Length=405, Percent_Identity=26.9135802469136, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=473, Percent_Identity=58.3509513742072, Blast_Score=556, Evalue=1e-159,
Organism=Saccharomyces cerevisiae, GI6323335, Length=478, Percent_Identity=27.6150627615063, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6322261, Length=405, Percent_Identity=27.1604938271605, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320440, Length=434, Percent_Identity=25.8064516129032, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI281365315, Length=485, Percent_Identity=24.7422680412371, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI17864292, Length=485, Percent_Identity=24.7422680412371, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI161076999, Length=421, Percent_Identity=24.7030878859857, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI28571643, Length=420, Percent_Identity=25.2380952380952, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24645686, Length=375, Percent_Identity=27.2, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI17137564, Length=478, Percent_Identity=25.5230125523013, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_ALISL (B6ELJ8)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002261906.1
- GeneID:   6988746
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0375
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50461; Mature: 50330

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNAKTLYEKVYDAHVAVAAKGETPILYIDRHLVHEVTSPQAFDGLREKGRNVRQVGKTF
CCCHHHHHHHHHHCEEEEEECCCCCEEEECHHHHHHCCCCHHHHHHHHCCCHHHHHHHHH
ATMDHNVSTQTKDINASGEMARIQMETLSKNCEEFGVTLYDLNHKYQGIVHVMGPELGIT
HHCCCCCCCCCCCCCCCCCEEEEEHHHHHCCHHHHCCEEEECCCCCCEEEEEECCCCCEE
LPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTLKQARAKTMKIEVKGKVTEGI
CCCEEEEECCCCCCCCCCCHHEEECCCHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCC
TAKDIVLAIIGKTTAAGGTGYVVEFCGEAITDLTMEGRMTVCNMAIELGAKAGLIAPDAT
CHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHEEHHHHHCCCCCCEECCCCC
TFDYITGRKFSPQGEDLTAAIEYWSSLKTDDDAKFDAVVSLDASEIKPQVTWGTNPGQVI
CCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCHHHCCCEEEECCCCCEEE
AIDQPIPAPESFSDPVEKVSAEKALAYMGLEAGKSLTDYNVDKVFVGSCTNSRIEDIRAA
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHH
AAVAKGNKVAAHVQALIVPGSEQVKAQAEKEGLDIIFKEAGFEWRLPGCSMCLAMNNDRL
HHHHCCCEEEEEEEEEEECCCHHHHHHHHHCCCEEEEECCCCEEECCCCEEEEEECCCCC
GPQERCASTSNRNFEGRQGRDGRTHLVSPAMAAAAAIAGHFVDIRTITEKA
CHHHHHCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHEEEEEEECCCC
>Mature Secondary Structure 
SNAKTLYEKVYDAHVAVAAKGETPILYIDRHLVHEVTSPQAFDGLREKGRNVRQVGKTF
CCHHHHHHHHHHCEEEEEECCCCCEEEECHHHHHHCCCCHHHHHHHHCCCHHHHHHHHH
ATMDHNVSTQTKDINASGEMARIQMETLSKNCEEFGVTLYDLNHKYQGIVHVMGPELGIT
HHCCCCCCCCCCCCCCCCCEEEEEHHHHHCCHHHHCCEEEECCCCCCEEEEEECCCCCEE
LPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTLKQARAKTMKIEVKGKVTEGI
CCCEEEEECCCCCCCCCCCHHEEECCCHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCC
TAKDIVLAIIGKTTAAGGTGYVVEFCGEAITDLTMEGRMTVCNMAIELGAKAGLIAPDAT
CHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHEEHHHHHCCCCCCEECCCCC
TFDYITGRKFSPQGEDLTAAIEYWSSLKTDDDAKFDAVVSLDASEIKPQVTWGTNPGQVI
CCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCHHHCCCEEEECCCCCEEE
AIDQPIPAPESFSDPVEKVSAEKALAYMGLEAGKSLTDYNVDKVFVGSCTNSRIEDIRAA
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHH
AAVAKGNKVAAHVQALIVPGSEQVKAQAEKEGLDIIFKEAGFEWRLPGCSMCLAMNNDRL
HHHHCCCEEEEEEEEEEECCCHHHHHHHHHCCCEEEEECCCCEEECCCCEEEEEECCCCC
GPQERCASTSNRNFEGRQGRDGRTHLVSPAMAAAAAIAGHFVDIRTITEKA
CHHHHHCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA