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The map label for this gene is leuA

Identifier: 209693980

GI number: 209693980

Start: 413018

End: 414565

Strand: Reverse

Name: leuA

Synonym: VSAL_I0377

Alternate gene names: 209693980

Gene position: 414565-413018 (Counterclockwise)

Preceding gene: 209693983

Following gene: 209693979

Centisome position: 12.47

GC content: 42.57

Gene sequence:

>1548_bases
ATGAGCGATCAAGTCATAATATTCGACACCACCTTAAGAGATGGCGAGCAAGCACTGTCTGCAAGCTTAACCGTAAAAGA
AAAATTGCAGATTGCGTTTGCGCTTGAGCGTCTTGGTGTTGATGTCATTGAAGCTGGCTTTCCAATTTCATCGCCAGGTG
ATTTTGAGTCGGTACGAACCATAGCAAAACACATCAAAAACAGTCGTATTTGTGCCCTTTCTCGTGCGGTTACTAAAGAT
ATCGATGTCGCAGCTGACGCATTAAAAGTCGCAGAAGCGTTTCGAATTCACACCTTCATTTCAACCTCAACGGTTCACGT
TCAAGATAAGTTACGTCGTAGTTATGATGACGTAGTAGAAATGGGCGTCAAAGCCGTTCAGCGTGCTCGTAAATACACCG
ATGATGTTGAATTTTCTTGTGAAGATGCAGGCCGTACTCCTATTGATAACCTATGTCGTATGGTAGAAGCCGCGATCAAC
GCAGGTGCAAATACCATCAATATTCCAGATACGGTGGGTTACACCATTCCTGGTGAATTTGGCGGTATTATTCAAACCCT
ATTTAATCGAGTTCCAAATATTGATAAAGCCATCATCTCAGTTCACTGTCATGATGATTTAGGTATGTCAGTTGCAAACT
CAATTGCGGCGGTTCAAGCAGGTGCTCGCCAAGTTGAAGGCACGATTAATGGTATTGGTGAGCGTGCAGGTAACTGTTCT
CTAGAAGAAATTGCAATGATTTTGCAAACTCGTTCAGAGTATCTAGGTGTTCATACTAGTCTTAAAAATGATGAAATCCA
TCGCACCAGTAAGCTAGTTAGTCAGCTTTGTAATATGCCAATTCAAAGTAATAAGGCTATTGTAGGCGCGAACGCGTTTA
GCCACTCTTCAGGCATCCACCAAGACGGAATGCTTAAGAATAAAAACACTTATGAAATCATGACGCCAGAATCTATTGGC
TTAAAAAACAAAGCGTTAAACCTAACCAGTCGTTCTGGCCGTGCCGCAGTTAAAAGTCACATGGATTCGATGGGTTATAA
AGCCGAAGAATATAATCTAGATACCTTGTATGCCGATTTCGTAAAACTGGCCGACCGTAAGGGCCAAGTATTTGATTACG
ATTTAGAAGCCCTAATGCACTTTGCTAATCTTCGCGATGAAGACGACTTTTATAAGTTGAACTACTTGAGCGTGCAATCT
GGCAGCGTCATGGCAACCACCAGTATCAAATTGCTTTGTGGCGACGAAGAAAAATGTGAAGCCGCTGTCGGTAACGGTCC
AGTAGATGCCCTTTATCAATGTATTTATAAGCTAACCGGTTATGAAATTGTACTGGATAAGTTTGACCTAACAGCGAAAG
GCGAAGGCGAGGACGGGTTAGGTCAAGCCGATATCATTGCAAATTACAAAGGCCGCAAGTACCACGGCACAGGCTTAGCT
ACCGATATTATTGAAGCATCAGGCCAAGCACTGCTTCACGTCATTAATAGCATTCACCGTGCTGATCAAATTGCAGAAAT
TAAGCAAAATAAAATTACGACGGTATAG

Upstream 100 bases:

>100_bases
AAAGAAGCCATAAACCAAATTTTAAAACCCGCTGAACATGGCGGGTTTTTTTATGGATAACCGCCGTCAAAGCGCACGAC
ATACACAAGGAAGAAAGCCC

Downstream 100 bases:

>100_bases
CACTAAATAAATTTAAATCAGGGCAAATACACCGTGCCTTGATTTTCCAAATTAAATAGAAAGATTAAAGGATTAACATG
ACGAATAAAACATACAAGAT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSDQVIIFDTTLRDGEQALSASLTVKEKLQIAFALERLGVDVIEAGFPISSPGDFESVRTIAKHIKNSRICALSRAVTKD
IDVAADALKVAEAFRIHTFISTSTVHVQDKLRRSYDDVVEMGVKAVQRARKYTDDVEFSCEDAGRTPIDNLCRMVEAAIN
AGANTINIPDTVGYTIPGEFGGIIQTLFNRVPNIDKAIISVHCHDDLGMSVANSIAAVQAGARQVEGTINGIGERAGNCS
LEEIAMILQTRSEYLGVHTSLKNDEIHRTSKLVSQLCNMPIQSNKAIVGANAFSHSSGIHQDGMLKNKNTYEIMTPESIG
LKNKALNLTSRSGRAAVKSHMDSMGYKAEEYNLDTLYADFVKLADRKGQVFDYDLEALMHFANLRDEDDFYKLNYLSVQS
GSVMATTSIKLLCGDEEKCEAAVGNGPVDALYQCIYKLTGYEIVLDKFDLTAKGEGEDGLGQADIIANYKGRKYHGTGLA
TDIIEASGQALLHVINSIHRADQIAEIKQNKITTV

Sequences:

>Translated_515_residues
MSDQVIIFDTTLRDGEQALSASLTVKEKLQIAFALERLGVDVIEAGFPISSPGDFESVRTIAKHIKNSRICALSRAVTKD
IDVAADALKVAEAFRIHTFISTSTVHVQDKLRRSYDDVVEMGVKAVQRARKYTDDVEFSCEDAGRTPIDNLCRMVEAAIN
AGANTINIPDTVGYTIPGEFGGIIQTLFNRVPNIDKAIISVHCHDDLGMSVANSIAAVQAGARQVEGTINGIGERAGNCS
LEEIAMILQTRSEYLGVHTSLKNDEIHRTSKLVSQLCNMPIQSNKAIVGANAFSHSSGIHQDGMLKNKNTYEIMTPESIG
LKNKALNLTSRSGRAAVKSHMDSMGYKAEEYNLDTLYADFVKLADRKGQVFDYDLEALMHFANLRDEDDFYKLNYLSVQS
GSVMATTSIKLLCGDEEKCEAAVGNGPVDALYQCIYKLTGYEIVLDKFDLTAKGEGEDGLGQADIIANYKGRKYHGTGLA
TDIIEASGQALLHVINSIHRADQIAEIKQNKITTV
>Mature_514_residues
SDQVIIFDTTLRDGEQALSASLTVKEKLQIAFALERLGVDVIEAGFPISSPGDFESVRTIAKHIKNSRICALSRAVTKDI
DVAADALKVAEAFRIHTFISTSTVHVQDKLRRSYDDVVEMGVKAVQRARKYTDDVEFSCEDAGRTPIDNLCRMVEAAINA
GANTINIPDTVGYTIPGEFGGIIQTLFNRVPNIDKAIISVHCHDDLGMSVANSIAAVQAGARQVEGTINGIGERAGNCSL
EEIAMILQTRSEYLGVHTSLKNDEIHRTSKLVSQLCNMPIQSNKAIVGANAFSHSSGIHQDGMLKNKNTYEIMTPESIGL
KNKALNLTSRSGRAAVKSHMDSMGYKAEEYNLDTLYADFVKLADRKGQVFDYDLEALMHFANLRDEDDFYKLNYLSVQSG
SVMATTSIKLLCGDEEKCEAAVGNGPVDALYQCIYKLTGYEIVLDKFDLTAKGEGEDGLGQADIIANYKGRKYHGTGLAT
DIIEASGQALLHVINSIHRADQIAEIKQNKITTV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=512, Percent_Identity=68.5546875, Blast_Score=741, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320071, Length=361, Percent_Identity=28.808864265928, Blast_Score=136, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6320019, Length=361, Percent_Identity=28.5318559556787, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324225, Length=560, Percent_Identity=24.4642857142857, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324682, Length=546, Percent_Identity=25.8241758241758, Blast_Score=125, Evalue=1e-29,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_ALISL (B6ELK0)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002261908.1
- ProteinModelPortal:   B6ELK0
- GeneID:   6988744
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0377
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 56211; Mature: 56080

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQVIIFDTTLRDGEQALSASLTVKEKLQIAFALERLGVDVIEAGFPISSPGDFESVRT
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHH
IAKHIKNSRICALSRAVTKDIDVAADALKVAEAFRIHTFISTSTVHVQDKLRRSYDDVVE
HHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEHHHHHHHHHHHHHH
MGVKAVQRARKYTDDVEFSCEDAGRTPIDNLCRMVEAAINAGANTINIPDTVGYTIPGEF
HHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCEECCCHH
GGIIQTLFNRVPNIDKAIISVHCHDDLGMSVANSIAAVQAGARQVEGTINGIGERAGNCS
HHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LEEIAMILQTRSEYLGVHTSLKNDEIHRTSKLVSQLCNMPIQSNKAIVGANAFSHSSGIH
HHHHHHHHHHHHHHHCEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
QDGMLKNKNTYEIMTPESIGLKNKALNLTSRSGRAAVKSHMDSMGYKAEEYNLDTLYADF
CCCCCCCCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHH
VKLADRKGQVFDYDLEALMHFANLRDEDDFYKLNYLSVQSGSVMATTSIKLLCGDEEKCE
HHHHCCCCCEEEHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEEEEEEEECCCHHHH
AAVGNGPVDALYQCIYKLTGYEIVLDKFDLTAKGEGEDGLGQADIIANYKGRKYHGTGLA
HHCCCCCHHHHHHHHHHHCCCEEEEEEECCEECCCCCCCCCCCCEEECCCCCEEECCCHH
TDIIEASGQALLHVINSIHRADQIAEIKQNKITTV
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SDQVIIFDTTLRDGEQALSASLTVKEKLQIAFALERLGVDVIEAGFPISSPGDFESVRT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHH
IAKHIKNSRICALSRAVTKDIDVAADALKVAEAFRIHTFISTSTVHVQDKLRRSYDDVVE
HHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEHHHHHHHHHHHHHH
MGVKAVQRARKYTDDVEFSCEDAGRTPIDNLCRMVEAAINAGANTINIPDTVGYTIPGEF
HHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCEECCCHH
GGIIQTLFNRVPNIDKAIISVHCHDDLGMSVANSIAAVQAGARQVEGTINGIGERAGNCS
HHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LEEIAMILQTRSEYLGVHTSLKNDEIHRTSKLVSQLCNMPIQSNKAIVGANAFSHSSGIH
HHHHHHHHHHHHHHHCEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
QDGMLKNKNTYEIMTPESIGLKNKALNLTSRSGRAAVKSHMDSMGYKAEEYNLDTLYADF
CCCCCCCCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHH
VKLADRKGQVFDYDLEALMHFANLRDEDDFYKLNYLSVQSGSVMATTSIKLLCGDEEKCE
HHHHCCCCCEEEHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEEEEEEEECCCHHHH
AAVGNGPVDALYQCIYKLTGYEIVLDKFDLTAKGEGEDGLGQADIIANYKGRKYHGTGLA
HHCCCCCHHHHHHHHHHHCCCEEEEEEECCEECCCCCCCCCCCCEEECCCCCEEECCCHH
TDIIEASGQALLHVINSIHRADQIAEIKQNKITTV
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA