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The map label for this gene is ksgA

Identifier: 209693971

GI number: 209693971

Start: 402206

End: 403039

Strand: Reverse

Name: ksgA

Synonym: VSAL_I0368

Alternate gene names: 209693971

Gene position: 403039-402206 (Counterclockwise)

Preceding gene: 209693972

Following gene: 209693970

Centisome position: 12.12

GC content: 42.33

Gene sequence:

>834_bases
ATGAGTACAAGAAATGATGTCCATTTAGGCCATAAAGCGAAGAAACGCTTTGGTCAAAACTTCTTAAACGACCCTTACGT
AATCGATGGAATCGTATCTGCAATCAACCCTCTGCCAGGTCAAAACCTTGTAGAAATTGGTCCAGGTCTTGGAGCAATCA
CTGAGCCTGTTGGCCGTGAAATAGACAAATTTACCGTTATCGAACTTGACCGTGATTTAGCCGCTCGTTTACGTACTCAC
CCTGAATTGGGCAGTAAATTAACTATTTATGAAGGCGATGCAATGCGCTTCGACTTCACGCAGCTTATTCAAGAAGGCAA
TAAATTACGTATTTTTGGTAACTTGCCATATAACATCTCTACACCATTAATGTTCCACCTTTTTGAATTTCATAAAGACA
TACAAGATATGCACTTTATGTTACAAAAAGAAGTGGTTAACCGCCTAGCGGCAGGTCCAGGAACTAAAGCTTATGGCCGC
CTAACAGTAATGGCTCAGTATTACTGTAAGGTAATGCCTGTACTTGAAGTGCCACCAACAGCGTTTGTTCCGCCACCGAA
GGTTGATTCTGCCGTCGTTCGTCTAGTGCCTTATGAGGTACTTCCGTTCCCTGCGAAGAACTTAAAATGGCTTGACCGCG
TATGTCGCGAAGGCTTCAACCAACGCCGCAAAACAGTACGAAACTGTTTCAAAGCACTATTAACAAAAGAACAATTGGAA
GCATTAGGTGTTAATCCTTCCCATCGTCCTGAAAACTTAACGTTAGAGCAGTTTGTTATCATGGCAAACTGGTTGAATGA
CAACTACCAAGCAGAATCTACAGAATCGGCTTAA

Upstream 100 bases:

>100_bases
TAGACCACGGTACTGCATTGGAATTAGCGGGTACTGGAAAAGCAGATGCAGGAAGCTTTATTACCGCACTTACCCATGCA
ATTGAACTGGTAGAAAATAA

Downstream 100 bases:

>100_bases
CTTAGTGGCTCATCCTTAACGAACACAATAAAATAAAAGGTGACTTCATTGTCACCTTTTTTCTATCTACTCCCATATTG
ATGTAAGGAAATATTCATGG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSTRNDVHLGHKAKKRFGQNFLNDPYVIDGIVSAINPLPGQNLVEIGPGLGAITEPVGREIDKFTVIELDRDLAARLRTH
PELGSKLTIYEGDAMRFDFTQLIQEGNKLRIFGNLPYNISTPLMFHLFEFHKDIQDMHFMLQKEVVNRLAAGPGTKAYGR
LTVMAQYYCKVMPVLEVPPTAFVPPPKVDSAVVRLVPYEVLPFPAKNLKWLDRVCREGFNQRRKTVRNCFKALLTKEQLE
ALGVNPSHRPENLTLEQFVIMANWLNDNYQAESTESA

Sequences:

>Translated_277_residues
MSTRNDVHLGHKAKKRFGQNFLNDPYVIDGIVSAINPLPGQNLVEIGPGLGAITEPVGREIDKFTVIELDRDLAARLRTH
PELGSKLTIYEGDAMRFDFTQLIQEGNKLRIFGNLPYNISTPLMFHLFEFHKDIQDMHFMLQKEVVNRLAAGPGTKAYGR
LTVMAQYYCKVMPVLEVPPTAFVPPPKVDSAVVRLVPYEVLPFPAKNLKWLDRVCREGFNQRRKTVRNCFKALLTKEQLE
ALGVNPSHRPENLTLEQFVIMANWLNDNYQAESTESA
>Mature_276_residues
STRNDVHLGHKAKKRFGQNFLNDPYVIDGIVSAINPLPGQNLVEIGPGLGAITEPVGREIDKFTVIELDRDLAARLRTHP
ELGSKLTIYEGDAMRFDFTQLIQEGNKLRIFGNLPYNISTPLMFHLFEFHKDIQDMHFMLQKEVVNRLAAGPGTKAYGRL
TVMAQYYCKVMPVLEVPPTAFVPPPKVDSAVVRLVPYEVLPFPAKNLKWLDRVCREGFNQRRKTVRNCFKALLTKEQLEA
LGVNPSHRPENLTLEQFVIMANWLNDNYQAESTESA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=279, Percent_Identity=31.5412186379928, Blast_Score=124, Evalue=7e-29,
Organism=Homo sapiens, GI7657198, Length=227, Percent_Identity=32.15859030837, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1786236, Length=265, Percent_Identity=66.0377358490566, Blast_Score=372, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI25141369, Length=306, Percent_Identity=26.1437908496732, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25146882, Length=219, Percent_Identity=32.8767123287671, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324989, Length=233, Percent_Identity=31.3304721030043, Blast_Score=99, Evalue=7e-22,
Organism=Drosophila melanogaster, GI21357273, Length=282, Percent_Identity=30.8510638297872, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21358017, Length=245, Percent_Identity=33.469387755102, Blast_Score=107, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_ALISL (B6EL48)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002261899.1
- GeneID:   6988241
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0368
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- ProtClustDB:   PRK00274
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 31449; Mature: 31318

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 20-20 BINDING 22-22 BINDING 47-47 BINDING 68-68 BINDING 93-93 BINDING 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTRNDVHLGHKAKKRFGQNFLNDPYVIDGIVSAINPLPGQNLVEIGPGLGAITEPVGRE
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHCCC
IDKFTVIELDRDLAARLRTHPELGSKLTIYEGDAMRFDFTQLIQEGNKLRIFGNLPYNIS
CCCEEEEEECHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCCEEEEEECCCCCCC
TPLMFHLFEFHKDIQDMHFMLQKEVVNRLAAGPGTKAYGRLTVMAQYYCKVMPVLEVPPT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
AFVPPPKVDSAVVRLVPYEVLPFPAKNLKWLDRVCREGFNQRRKTVRNCFKALLTKEQLE
CCCCCCCCCHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGVNPSHRPENLTLEQFVIMANWLNDNYQAESTESA
HHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
STRNDVHLGHKAKKRFGQNFLNDPYVIDGIVSAINPLPGQNLVEIGPGLGAITEPVGRE
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHCCC
IDKFTVIELDRDLAARLRTHPELGSKLTIYEGDAMRFDFTQLIQEGNKLRIFGNLPYNIS
CCCEEEEEECHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCCEEEEEECCCCCCC
TPLMFHLFEFHKDIQDMHFMLQKEVVNRLAAGPGTKAYGRLTVMAQYYCKVMPVLEVPPT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
AFVPPPKVDSAVVRLVPYEVLPFPAKNLKWLDRVCREGFNQRRKTVRNCFKALLTKEQLE
CCCCCCCCCHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGVNPSHRPENLTLEQFVIMANWLNDNYQAESTESA
HHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA