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The map label for this gene is 209693786

Identifier: 209693786

GI number: 209693786

Start: 209067

End: 210140

Strand: Reverse

Name: 209693786

Synonym: VSAL_I0169

Alternate gene names: NA

Gene position: 210140-209067 (Counterclockwise)

Preceding gene: 209693787

Following gene: 209693785

Centisome position: 6.32

GC content: 40.13

Gene sequence:

>1074_bases
ATGACCCTAATTATTGCAGAAGCTGGCGTTAACCATAACGGTGATGAAAATCTGGCCTTTCAACTTGTTGATGCGGCTTA
TCACGCAGGTGCAGACATTGTTAAATTTCAAACCTTTAAAGCAAAAAATCTAGTAACGGAAGATGCAGTTCAAGCAGAGT
ATCAAGTTACCAATACACAAAAGCAAGAATCACAATTAGCAATGTTAAGTCGATTAGAGCTTTCGTATGATATTCACCAT
CAGCTAGTTAAGCATTGTGAATCGTTAGGTATTGAATTTCTTTCAACCGCGTTTGATTCAGAAAGCCTTGATTTTTTAGT
AAATGATTTAGGGTTAACTCGTCTTAAAATTCCATCAGGTGAAATTACCAATGCCCCATTGGTTTTAGAGCACGCTCGTA
CTGGATGTGATTTAATTGTCTCTACTGGCATGGCAACCTTGTCAGAAATTGAAGCAGTATTAGGCGTCATTGCTTTTGGT
TATGTTGCAGATAAAACAGAAGAGCCAAGTGAGTTAGCTTTTCAGCAAGCTTATGCTTCAGAGCAAGGTCAGAAAGCACT
AAAGAAAAAAGTGACAATATTGCATTGCACTACAGAATACCCAGCTCCAATGGTTGAAATCAATCTCAAAGCAATGGATA
CATTAGGTCGTGCATTTGAGTTACCTGCAGGCTATTCCGATCACAGTGCAGGGATCACCATTCCAATTGCTGCTGTTGCT
CGTGGTGCGGTTTTAATTGAAAAGCACTTTACGTTAGACCAAAACATGGAAGGCCCAGACCATAAAGCCTCTTTAGAGCC
TCAAGAATTAACAGCTATGGTAAGCGCAATACGCCAAGTTGAAGTTGCTTTAGGTGTGGGAGTGAAAAGTCCAACGGTAT
CAGAAGTAAAAAACAAAGCCGTTGCGCGTAAAAGTTTAGTGGCAGCGAAAGCGATTCAACAAGGTGATCTTATTACAGAA
GATAACCTTACAATTAAACGCCCAGGATCAGGCATGTCGCCATACCACTACTGGGCTCTATTGAATCAACCTGCAAGCCA
AGATTATAAAGCAGGAGATTTAATTGTTGAGTAA

Upstream 100 bases:

>100_bases
GATACTAGCGGTAAAGTGATTGAAATGATTAAATCGTTGCAATTCACACCAAGCAAAGCGTTTTACGATATAACTATTAA
CGATGTAGAGTGAGAGAGTC

Downstream 100 bases:

>100_bases
GACAGAGTTAAAGCCTCTGGTTGTGATTGGTGGAGGAGGTCACGCAAGTGTCTTAGTTGATATCCTTCGTTCACAACAGC
GTGAAATTCTTGCTATTGTT

Product: N-acetylneuraminic acid synthase

Products: phosphate; N-acetylneuraminate

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MTLIIAEAGVNHNGDENLAFQLVDAAYHAGADIVKFQTFKAKNLVTEDAVQAEYQVTNTQKQESQLAMLSRLELSYDIHH
QLVKHCESLGIEFLSTAFDSESLDFLVNDLGLTRLKIPSGEITNAPLVLEHARTGCDLIVSTGMATLSEIEAVLGVIAFG
YVADKTEEPSELAFQQAYASEQGQKALKKKVTILHCTTEYPAPMVEINLKAMDTLGRAFELPAGYSDHSAGITIPIAAVA
RGAVLIEKHFTLDQNMEGPDHKASLEPQELTAMVSAIRQVEVALGVGVKSPTVSEVKNKAVARKSLVAAKAIQQGDLITE
DNLTIKRPGSGMSPYHYWALLNQPASQDYKAGDLIVE

Sequences:

>Translated_357_residues
MTLIIAEAGVNHNGDENLAFQLVDAAYHAGADIVKFQTFKAKNLVTEDAVQAEYQVTNTQKQESQLAMLSRLELSYDIHH
QLVKHCESLGIEFLSTAFDSESLDFLVNDLGLTRLKIPSGEITNAPLVLEHARTGCDLIVSTGMATLSEIEAVLGVIAFG
YVADKTEEPSELAFQQAYASEQGQKALKKKVTILHCTTEYPAPMVEINLKAMDTLGRAFELPAGYSDHSAGITIPIAAVA
RGAVLIEKHFTLDQNMEGPDHKASLEPQELTAMVSAIRQVEVALGVGVKSPTVSEVKNKAVARKSLVAAKAIQQGDLITE
DNLTIKRPGSGMSPYHYWALLNQPASQDYKAGDLIVE
>Mature_356_residues
TLIIAEAGVNHNGDENLAFQLVDAAYHAGADIVKFQTFKAKNLVTEDAVQAEYQVTNTQKQESQLAMLSRLELSYDIHHQ
LVKHCESLGIEFLSTAFDSESLDFLVNDLGLTRLKIPSGEITNAPLVLEHARTGCDLIVSTGMATLSEIEAVLGVIAFGY
VADKTEEPSELAFQQAYASEQGQKALKKKVTILHCTTEYPAPMVEINLKAMDTLGRAFELPAGYSDHSAGITIPIAAVAR
GAVLIEKHFTLDQNMEGPDHKASLEPQELTAMVSAIRQVEVALGVGVKSPTVSEVKNKAVARKSLVAAKAIQQGDLITED
NLTIKRPGSGMSPYHYWALLNQPASQDYKAGDLIVE

Specific function: Unknown

COG id: COG2089

COG function: function code M; Sialic acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis spsE [H]

Homologues:

Organism=Homo sapiens, GI12056473, Length=330, Percent_Identity=31.5151515151515, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI21357081, Length=281, Percent_Identity=32.7402135231317, Blast_Score=142, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006190
- InterPro:   IPR013785
- InterPro:   IPR013132
- InterPro:   IPR013974 [H]

Pfam domain/function: PF03102 NeuB; PF08666 SAF [H]

EC number: 2.5.1.56

Molecular weight: Translated: 38674; Mature: 38543

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50844 AFP_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLIIAEAGVNHNGDENLAFQLVDAAYHAGADIVKFQTFKAKNLVTEDAVQAEYQVTNTQ
CEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEHHHCCHHHHHHHHHEEECCCH
KQESQLAMLSRLELSYDIHHQLVKHCESLGIEFLSTAFDSESLDFLVNDLGLTRLKIPSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHCCCCEEEECCCC
EITNAPLVLEHARTGCDLIVSTGMATLSEIEAVLGVIAFGYVADKTEEPSELAFQQAYAS
CCCCCCEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
EQGQKALKKKVTILHCTTEYPAPMVEINLKAMDTLGRAFELPAGYSDHSAGITIPIAAVA
HHHHHHHHHCEEEEEEECCCCCCEEEEEHHHHHHHHHHEECCCCCCCCCCCEEEEHHHHH
RGAVLIEKHFTLDQNMEGPDHKASLEPQELTAMVSAIRQVEVALGVGVKSPTVSEVKNKA
CCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VARKSLVAAKAIQQGDLITEDNLTIKRPGSGMSPYHYWALLNQPASQDYKAGDLIVE
HHHHHHHHHHHHHCCCCEECCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCEEEC
>Mature Secondary Structure 
TLIIAEAGVNHNGDENLAFQLVDAAYHAGADIVKFQTFKAKNLVTEDAVQAEYQVTNTQ
EEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEHHHCCHHHHHHHHHEEECCCH
KQESQLAMLSRLELSYDIHHQLVKHCESLGIEFLSTAFDSESLDFLVNDLGLTRLKIPSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHCCCCEEEECCCC
EITNAPLVLEHARTGCDLIVSTGMATLSEIEAVLGVIAFGYVADKTEEPSELAFQQAYAS
CCCCCCEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
EQGQKALKKKVTILHCTTEYPAPMVEINLKAMDTLGRAFELPAGYSDHSAGITIPIAAVA
HHHHHHHHHCEEEEEEECCCCCCEEEEEHHHHHHHHHHEECCCCCCCCCCCEEEEHHHHH
RGAVLIEKHFTLDQNMEGPDHKASLEPQELTAMVSAIRQVEVALGVGVKSPTVSEVKNKA
CCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VARKSLVAAKAIQQGDLITEDNLTIKRPGSGMSPYHYWALLNQPASQDYKAGDLIVE
HHHHHHHHHHHHHCCCCEECCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoenolpyruvate; N-acetyl-D-mannosamine; H2O

Specific reaction: phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O = phosphate + N-acetylneuraminate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]