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The map label for this gene is asnS [H]

Identifier: 197117272

GI number: 197117272

Start: 1035422

End: 1036807

Strand: Reverse

Name: asnS [H]

Synonym: Gbem_0882

Alternate gene names: 197117272

Gene position: 1036807-1035422 (Counterclockwise)

Preceding gene: 197117275

Following gene: 197117267

Centisome position: 22.47

GC content: 63.78

Gene sequence:

>1386_bases
ATGACAGCGAGAACCAGGGTAAAACAGGCACTGAGCGAGGGACGGCCGGGCGAGACTTTACTGCTCAAGGGGTGGGCGAG
GACGGTGCGGACCGGCAAGGGAGTGACCTTCATCTCCCTGAACGACGGATCGACACTGGCGGGGATACAGGTGGTGGCCG
AGCCGGAGCTTGCCAATTATGCAGAGGTTGCCGCGCTTGGCACAGGAAGCGCGCTCACCGTGACCGGCGTGCTGCAGGAG
TCGCCTGCAGCGGGGCAGGAACTGGAGCTGAAGGCGCTCTCGGTAGAAGTGGTGGGTACGGCGGACCAGGAATACCCGAT
GCAGAAAAAACGGCACAGCTTCGAATACCTGCGCACCATCGCCCACATGAGGGTGCGCAGCAACACCTTCGGCGCCGTGT
TCCGCGTCCGGTCGGCGCTGGCCCAGGAGGTGCACCGCTTCTTCGCCGAGCGCGGCTTTCTATACGTGCACACCCCCATC
ATCACTGCCAACGACTGCGAGGGGGCGGGGGAACTCTTCCGCGTCACCACGCTGGACCTGAACAACCCGCCCCGGGTCGA
GGGTGAGATCGACTTCGCGCAGGACTTCTTCGCTAAAGCCACCGGCCTTACCGTGAGCGGGCAACTGGAGGGGGAACTCT
TCGCCCAGGCCTTCTCCGACATCTACACCTTCGGCCCCACCTTCCGCGCGGAAAACTCCAACACGGCGCGGCACGCCGCC
GAGTTCTGGATGATCGAGCCGGAGATGGCCTTCGCCGACCTCTCCGACGACGCCGACTTGGCCGAGGCCTTTCTGAAAAG
GCTCTGCCGCTTCGCGCTGGACAACTGCGACGACGACATGCGGTTCTTCAACGCACAGATCGACAAGGGGCTCATCGAGC
GGATAGAGGGGGTGGCGCAGGCCTCCTTCGCCAGGATGGATTACGGCGAGGCGATCGAGCGGCTCAAGAAGGCGCCGGTC
TCCTTCCAGTTTCCGGTGGAGTGGGGGCTCGACCTGCAGTCCGAGCATGAGCGCTACCTGACCGAGCAGGTGGTCGGCGG
CCCGGTCTTCATAATGAACTACCCCAAGGACATCAAGGCATTTTACATGAGGCTCAACGACGACGGCAGGACCGTCGCCG
CCATGGACCTCTTGGTCCCCAAGGTGGGAGAGATCATCGGGGGGAGCCAGCGCGAGGAGCGGTTAGAGCCGCTGATCGGC
CGGATGATGGAGGTCGGGATCGAGCCGGAGTCGCTTTCCTGGTACCTCGACACCAGGCGCTGGGGCTCGACCCCGCATGC
GGGGTTCGGCCTCGGGTTCGAAAGGCTGATCATGTACCTGACCGGGATGGAGAACATCAGGGACGTGATTCCTTTCCCCA
GGACGCCGAGGCACGCGGAATTCTAG

Upstream 100 bases:

>100_bases
AACCTATACGAACCCCGGGTGGATTGTCAACTATGCTTTTGCTGTGGCCTGACCTGTATGCTATAAATGGTCATTAAAAT
CTACCAGCGGAGCGGCTCAC

Downstream 100 bases:

>100_bases
GATAGCAATTAACAATTGACGATTGACAATTGACAACGAAACACCACCTACCGAAGGCTCAACGAAAAGGGGCCGGCGGG
TCAGCTTGTAGCTGCTTTGC

Product: asparaginyl-tRNA synthetase

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MTARTRVKQALSEGRPGETLLLKGWARTVRTGKGVTFISLNDGSTLAGIQVVAEPELANYAEVAALGTGSALTVTGVLQE
SPAAGQELELKALSVEVVGTADQEYPMQKKRHSFEYLRTIAHMRVRSNTFGAVFRVRSALAQEVHRFFAERGFLYVHTPI
ITANDCEGAGELFRVTTLDLNNPPRVEGEIDFAQDFFAKATGLTVSGQLEGELFAQAFSDIYTFGPTFRAENSNTARHAA
EFWMIEPEMAFADLSDDADLAEAFLKRLCRFALDNCDDDMRFFNAQIDKGLIERIEGVAQASFARMDYGEAIERLKKAPV
SFQFPVEWGLDLQSEHERYLTEQVVGGPVFIMNYPKDIKAFYMRLNDDGRTVAAMDLLVPKVGEIIGGSQREERLEPLIG
RMMEVGIEPESLSWYLDTRRWGSTPHAGFGLGFERLIMYLTGMENIRDVIPFPRTPRHAEF

Sequences:

>Translated_461_residues
MTARTRVKQALSEGRPGETLLLKGWARTVRTGKGVTFISLNDGSTLAGIQVVAEPELANYAEVAALGTGSALTVTGVLQE
SPAAGQELELKALSVEVVGTADQEYPMQKKRHSFEYLRTIAHMRVRSNTFGAVFRVRSALAQEVHRFFAERGFLYVHTPI
ITANDCEGAGELFRVTTLDLNNPPRVEGEIDFAQDFFAKATGLTVSGQLEGELFAQAFSDIYTFGPTFRAENSNTARHAA
EFWMIEPEMAFADLSDDADLAEAFLKRLCRFALDNCDDDMRFFNAQIDKGLIERIEGVAQASFARMDYGEAIERLKKAPV
SFQFPVEWGLDLQSEHERYLTEQVVGGPVFIMNYPKDIKAFYMRLNDDGRTVAAMDLLVPKVGEIIGGSQREERLEPLIG
RMMEVGIEPESLSWYLDTRRWGSTPHAGFGLGFERLIMYLTGMENIRDVIPFPRTPRHAEF
>Mature_460_residues
TARTRVKQALSEGRPGETLLLKGWARTVRTGKGVTFISLNDGSTLAGIQVVAEPELANYAEVAALGTGSALTVTGVLQES
PAAGQELELKALSVEVVGTADQEYPMQKKRHSFEYLRTIAHMRVRSNTFGAVFRVRSALAQEVHRFFAERGFLYVHTPII
TANDCEGAGELFRVTTLDLNNPPRVEGEIDFAQDFFAKATGLTVSGQLEGELFAQAFSDIYTFGPTFRAENSNTARHAAE
FWMIEPEMAFADLSDDADLAEAFLKRLCRFALDNCDDDMRFFNAQIDKGLIERIEGVAQASFARMDYGEAIERLKKAPVS
FQFPVEWGLDLQSEHERYLTEQVVGGPVFIMNYPKDIKAFYMRLNDDGRTVAAMDLLVPKVGEIIGGSQREERLEPLIGR
MMEVGIEPESLSWYLDTRRWGSTPHAGFGLGFERLIMYLTGMENIRDVIPFPRTPRHAEF

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI226491502, Length=462, Percent_Identity=44.5887445887446, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI4758762, Length=460, Percent_Identity=29.3478260869565, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI45439306, Length=493, Percent_Identity=24.9492900608519, Blast_Score=122, Evalue=1e-27,
Organism=Escherichia coli, GI1787161, Length=447, Percent_Identity=57.2706935123042, Blast_Score=522, Evalue=1e-149,
Organism=Escherichia coli, GI1789256, Length=385, Percent_Identity=23.6363636363636, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17556372, Length=436, Percent_Identity=34.1743119266055, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI71984122, Length=448, Percent_Identity=30.3571428571429, Blast_Score=195, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6319872, Length=464, Percent_Identity=37.7155172413793, Blast_Score=295, Evalue=9e-81,
Organism=Saccharomyces cerevisiae, GI6321807, Length=468, Percent_Identity=29.2735042735043, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6323011, Length=355, Percent_Identity=28.4507042253521, Blast_Score=116, Evalue=7e-27,
Organism=Drosophila melanogaster, GI19922416, Length=466, Percent_Identity=34.9785407725322, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI19921528, Length=451, Percent_Identity=28.3813747228381, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI17136276, Length=356, Percent_Identity=26.9662921348315, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: =6.1.1.22 [H]

Molecular weight: Translated: 51509; Mature: 51378

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARTRVKQALSEGRPGETLLLKGWARTVRTGKGVTFISLNDGSTLAGIQVVAEPELANY
CCCHHHHHHHHHCCCCCCEEEEEHHHHHHHCCCCEEEEEECCCCEEEEEEEEECCCHHCH
AEVAALGTGSALTVTGVLQESPAAGQELELKALSVEVVGTADQEYPMQKKRHSFEYLRTI
HHHHEECCCCEEEEEEEECCCCCCCCCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHH
AHMRVRSNTFGAVFRVRSALAQEVHRFFAERGFLYVHTPIITANDCEGAGELFRVTTLDL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECEEEECCCCCCCCEEEEEEEEC
NNPPRVEGEIDFAQDFFAKATGLTVSGQLEGELFAQAFSDIYTFGPTFRAENSNTARHAA
CCCCCCCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCEECCCCCCHHHHH
EFWMIEPEMAFADLSDDADLAEAFLKRLCRFALDNCDDDMRFFNAQIDKGLIERIEGVAQ
HHEEECCCHHEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ASFARMDYGEAIERLKKAPVSFQFPVEWGLDLQSEHERYLTEQVVGGPVFIMNYPKDIKA
HHHHHCCHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCHHHEE
FYMRLNDDGRTVAAMDLLVPKVGEIIGGSQREERLEPLIGRMMEVGIEPESLSWYLDTRR
EEEEECCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
WGSTPHAGFGLGFERLIMYLTGMENIRDVIPFPRTPRHAEF
CCCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TARTRVKQALSEGRPGETLLLKGWARTVRTGKGVTFISLNDGSTLAGIQVVAEPELANY
CCHHHHHHHHHCCCCCCEEEEEHHHHHHHCCCCEEEEEECCCCEEEEEEEEECCCHHCH
AEVAALGTGSALTVTGVLQESPAAGQELELKALSVEVVGTADQEYPMQKKRHSFEYLRTI
HHHHEECCCCEEEEEEEECCCCCCCCCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHH
AHMRVRSNTFGAVFRVRSALAQEVHRFFAERGFLYVHTPIITANDCEGAGELFRVTTLDL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECEEEECCCCCCCCEEEEEEEEC
NNPPRVEGEIDFAQDFFAKATGLTVSGQLEGELFAQAFSDIYTFGPTFRAENSNTARHAA
CCCCCCCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCEECCCCCCHHHHH
EFWMIEPEMAFADLSDDADLAEAFLKRLCRFALDNCDDDMRFFNAQIDKGLIERIEGVAQ
HHEEECCCHHEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ASFARMDYGEAIERLKKAPVSFQFPVEWGLDLQSEHERYLTEQVVGGPVFIMNYPKDIKA
HHHHHCCHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCHHHEE
FYMRLNDDGRTVAAMDLLVPKVGEIIGGSQREERLEPLIGRMMEVGIEPESLSWYLDTRR
EEEEECCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
WGSTPHAGFGLGFERLIMYLTGMENIRDVIPFPRTPRHAEF
CCCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA